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Literature summary extracted from

  • Chen, M.; Wangtrakuldee, P.; Zang, T.; Duan, L.; Gathercole, L.L.; Tomlinson, J.W.; Penning, T.M.
    Human and murine steroid 5beta-reductases (AKR1D1 and AKR1D4) insights into the role of the catalytic glutamic acid (2019), Chem. Biol. Interact., 305, 163-170 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.3 expression in Escherichia coli Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.3 0.0057
-
 4-androstene-3,17-dione isoform AKR1D4L, pH 7, 37°C Mus musculus
1.3.1.3 0.0071
-
 cortisol isoform AKR1D4L, pH 7, 37°C Mus musculus
1.3.1.3 0.008
-
 4-androstene-3,17-dione isoform AKR1D4S, pH 7, 37°C Mus musculus
1.3.1.3 0.0114
-
 cortisol isoform AKR1D4S, pH 7, 37°C Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.3 Mus musculus Q8VCX1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.3 recombinant protein Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.3 liver expression of total AKR1D4 transcripts is 100fold higher in liver versus testis Mus musculus
-
1.3.1.3 testis expression of total AKR1D4 transcripts is 100fold higher in liver versus testis Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.3 4-androstene-3,17-dione + NADPH + H+
-
 Mus musculus 5beta-androstan-3,15-dione + 3alpha-hydroxy-5beta-androstan-17-one + NADP+
-
 ?
1.3.1.3 cortisol + NADPH + H+
-
 Mus musculus 11beta,17,21-trihydroxy-5beta-pregnane-3,20-dione + NADP+
-
 ?
1.3.1.3 progesterone + NADPH + H+
-
 Mus musculus 5beta-pregnane-3,20-dione + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.3 More there exists a long-form, with an 18 amino-acid extension at the N-terminus (AKR1D4L) and a short-form (AKR1D4S) Mus musculus

Synonyms

EC Number Synonyms Comment Organism
1.3.1.3 AKR1D1
-
 Mus musculus
1.3.1.3 AKR1D4
-
 Mus musculus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.1.3 0.05
-
 4-androstene-3,17-dione isoform AKR1D4L, pH 7, 37°C Mus musculus
1.3.1.3 0.08
-
 cortisol isoform AKR1D4L, pH 7, 37°C Mus musculus
1.3.1.3 0.09
-
 4-androstene-3,17-dione isoform AKR1D4S, pH 7, 37°C Mus musculus
1.3.1.3 0.13
-
 cortisol isoform AKR1D4S, pH 7, 37°C Mus musculus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.3.1.3 0.93
-
 4-androstene-3,17-dione isoform AKR1D4L, pH 7, 37°C Mus musculus
1.3.1.3 1
-
 4-androstene-3,17-dione isoform AKR1D4S, pH 7, 37°C Mus musculus
1.3.1.3 10.5
-
 cortisol isoform AKR1D4S, pH 7, 37°C Mus musculus
1.3.1.3 13
-
 cortisol isoform AKR1D4L, pH 7, 37°C Mus musculus