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Literature summary extracted from

  • Pathiraja, D.; Kim, K.H.; Choi, I.G.
    Rapid and robust enzymatic sensing and quantitation of 3,6-anhydro-L-galactose in a heterogeneous sugar mixture (2017), Carbohydr. Res., 446-447, 13-18 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.92 EDTA 132.2% activity at 1 mM Vibrio sp. EJY3

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.92 Ca2+ 80.8% residual activity at 1 mM Vibrio sp. EJY3
1.2.1.92 Cd2+ complete inhibition at 1 mM Vibrio sp. EJY3
1.2.1.92 Co2+ complete inhibition at 1 mM Vibrio sp. EJY3
1.2.1.92 Cu2+ complete inhibition at 1 mM Vibrio sp. EJY3
1.2.1.92 Mn2+ 68.2% residual activity at 1 mM Vibrio sp. EJY3
1.2.1.92 additional information not inhibited by EDTA Vibrio sp. EJY3
1.2.1.92 Ni2+ complete inhibition at 1 mM Vibrio sp. EJY3
1.2.1.92 Zn2+ complete inhibition at 1 mM Vibrio sp. EJY3

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.92 0.19
-
 3,6-anhydro-alpha-L-galactopyranose at pH 8.0 and 20°C Vibrio sp. EJY3

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.92 additional information Mg2+, Na+ and K+ have no effect on the enzyme activity Vibrio sp. EJY3

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.92 3,6-anhydro-alpha-L-galactopyranose + NADP+ + H2O Vibrio sp. EJY3 the enzyme is highly specific to 3,6-anhydro-alpha-L-galactopyranose 3,6-anhydro-L-galactonate + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.92 Vibrio sp. EJY3 H2IFE7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.92 HisTrap column chromatography Vibrio sp. EJY3

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.92 3,6-anhydro-alpha-L-galactopyranose + NADP+ + H2O the enzyme is highly specific to 3,6-anhydro-alpha-L-galactopyranose Vibrio sp. EJY3 3,6-anhydro-L-galactonate + NADPH + H+
-
 ?
1.2.1.92 additional information no activity with D-glucose, D-galactose, D-fructose, sucrose, maltose, arabinose, xylose, agarohexose, agarotetrose and neoagarobiose Vibrio sp. EJY3 ?
-
-

Synonyms

EC Number Synonyms Comment Organism
1.2.1.92 AHGD
-
 Vibrio sp. EJY3
1.2.1.92 anhydrogalactose dehydrogenase
-
 Vibrio sp. EJY3

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.92 20
-
-
 Vibrio sp. EJY3

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.92 20 50 the enzyme shows 50% activity after 12 h at 20°C and pH 8.0. At 50°C, the half-life of the enzyme is less than 10 min Vibrio sp. EJY3

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.92 0.006
-
 3,6-anhydro-alpha-L-galactopyranose at pH 8.0 and 20°C Vibrio sp. EJY3

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.92 8
-
-
 Vibrio sp. EJY3

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.92 6 12 the enzyme shows over 90% of its maximum within a small pH range from 8.0 to 11.0 and more than 60% activity between pH 6.0 and 12.0. At pH values lower than 5.0, activity of the enzyme is drastically reduced Vibrio sp. EJY3

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.2.1.92 8
-
 the enzyme shows 50% activity after 12 h at 20°C and pH 8.0 Vibrio sp. EJY3

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.92 NADP+ dependent on Vibrio sp. EJY3

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.2.1.92 0.032
-
 3,6-anhydro-alpha-L-galactopyranose at pH 8.0 and 20°C Vibrio sp. EJY3