Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Matelska, D.; Shabalin, I.; Jablonska, J.; Domagalski, M.; Kutner, J.; Ginalski, K.; Minor, W.
    Classification, substrate specificity and structural features of D-2-hydroxyacid dehydrogenases 2HADH knowledgebase (2018), BMC Evol. Biol., 18, 199 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.272 three crystal structures of DDH_HALMT are solved in complex with combinations of NAD+, NADP+, NADPH, 2-ketohexanoic acid, and 2-hydroxyhexanoic acid (PDB IDs are 5mha, 5mh5, 5mh6) Haloferax mediterranei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.272 2-oxoisoleucine + NADPH + H+ Haloferax mediterranei
-
 2-D-hydroxyisoleucine + NADP+
-
 r
1.1.1.272 2-oxoisoleucine + NADPH + H+ Haloferax mediterranei ATCC 33500
-
 2-D-hydroxyisoleucine + NADP+
-
 r
1.1.1.272 2-oxoisoleucine + NADPH + H+ Haloferax mediterranei NBRC 14739
-
 2-D-hydroxyisoleucine + NADP+
-
 r
1.1.1.272 2-oxoisoleucine + NADPH + H+ Haloferax mediterranei JCM 8866
-
 2-D-hydroxyisoleucine + NADP+
-
 r
1.1.1.272 2-oxoisoleucine + NADPH + H+ Haloferax mediterranei R-4
-
 2-D-hydroxyisoleucine + NADP+
-
 r
1.1.1.272 2-oxoisoleucine + NADPH + H+ Haloferax mediterranei DSM 1411
-
 2-D-hydroxyisoleucine + NADP+
-
 r
1.1.1.272 2-oxoisoleucine + NADPH + H+ Haloferax mediterranei NCIMB 2177
-
 2-D-hydroxyisoleucine + NADP+
-
 r
1.1.1.272 an (R)-2-hydroxycarboxylate + NADP+ Haloferax mediterranei
-
 a 2-oxocarboxylate + NADPH + H+
-
 r
1.1.1.272 an (R)-2-hydroxycarboxylate + NADP+ Haloferax mediterranei ATCC 33500
-
 a 2-oxocarboxylate + NADPH + H+
-
 r
1.1.1.272 an (R)-2-hydroxycarboxylate + NADP+ Haloferax mediterranei NBRC 14739
-
 a 2-oxocarboxylate + NADPH + H+
-
 r
1.1.1.272 an (R)-2-hydroxycarboxylate + NADP+ Haloferax mediterranei JCM 8866
-
 a 2-oxocarboxylate + NADPH + H+
-
 r
1.1.1.272 an (R)-2-hydroxycarboxylate + NADP+ Haloferax mediterranei R-4
-
 a 2-oxocarboxylate + NADPH + H+
-
 r
1.1.1.272 an (R)-2-hydroxycarboxylate + NADP+ Haloferax mediterranei DSM 1411
-
 a 2-oxocarboxylate + NADPH + H+
-
 r
1.1.1.272 an (R)-2-hydroxycarboxylate + NADP+ Haloferax mediterranei NCIMB 2177
-
 a 2-oxocarboxylate + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.272 Haloferax mediterranei Q2VEQ7
-
-
1.1.1.272 Haloferax mediterranei ATCC 33500 Q2VEQ7
-
-
1.1.1.272 Haloferax mediterranei DSM 1411 Q2VEQ7
-
-
1.1.1.272 Haloferax mediterranei JCM 8866 Q2VEQ7
-
-
1.1.1.272 Haloferax mediterranei NBRC 14739 Q2VEQ7
-
-
1.1.1.272 Haloferax mediterranei NCIMB 2177 Q2VEQ7
-
-
1.1.1.272 Haloferax mediterranei R-4 Q2VEQ7
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.272 2-oxo-3-methylvalerate + NADPH + H+
-
 Haloferax mediterranei 2-D-hydroxy-3-methylvalerate + NADP+
-
 r
1.1.1.272 2-oxo-3-methylvalerate + NADPH + H+
-
 Haloferax mediterranei ATCC 33500 2-D-hydroxy-3-methylvalerate + NADP+
-
 r
1.1.1.272 2-oxo-3-methylvalerate + NADPH + H+
-
 Haloferax mediterranei NBRC 14739 2-D-hydroxy-3-methylvalerate + NADP+
-
 r
1.1.1.272 2-oxo-3-methylvalerate + NADPH + H+
-
 Haloferax mediterranei JCM 8866 2-D-hydroxy-3-methylvalerate + NADP+
-
 r
1.1.1.272 2-oxo-3-methylvalerate + NADPH + H+
-
 Haloferax mediterranei R-4 2-D-hydroxy-3-methylvalerate + NADP+
-
 r
1.1.1.272 2-oxo-3-methylvalerate + NADPH + H+
-
 Haloferax mediterranei DSM 1411 2-D-hydroxy-3-methylvalerate + NADP+
-
 r
1.1.1.272 2-oxo-3-methylvalerate + NADPH + H+
-
 Haloferax mediterranei NCIMB 2177 2-D-hydroxy-3-methylvalerate + NADP+
-
 r
1.1.1.272 2-oxobutyrate + NADPH + H+
-
 Haloferax mediterranei 2-D-hydroxybutyrate + NADP+
-
 r
1.1.1.272 2-oxoisocaproate + NADPH + H+
-
 Haloferax mediterranei 2-hydroxyisocaproate + NADP+
-
 r
1.1.1.272 2-oxoisoleucine + NADPH + H+
-
 Haloferax mediterranei 2-D-hydroxyisoleucine + NADP+
-
 r
1.1.1.272 2-oxoisoleucine + NADPH + H+
-
 Haloferax mediterranei ATCC 33500 2-D-hydroxyisoleucine + NADP+
-
 r
1.1.1.272 2-oxoisoleucine + NADPH + H+
-
 Haloferax mediterranei NBRC 14739 2-D-hydroxyisoleucine + NADP+
-
 r
1.1.1.272 2-oxoisoleucine + NADPH + H+
-
 Haloferax mediterranei JCM 8866 2-D-hydroxyisoleucine + NADP+
-
 r
1.1.1.272 2-oxoisoleucine + NADPH + H+
-
 Haloferax mediterranei R-4 2-D-hydroxyisoleucine + NADP+
-
 r
1.1.1.272 2-oxoisoleucine + NADPH + H+
-
 Haloferax mediterranei DSM 1411 2-D-hydroxyisoleucine + NADP+
-
 r
1.1.1.272 2-oxoisoleucine + NADPH + H+
-
 Haloferax mediterranei NCIMB 2177 2-D-hydroxyisoleucine + NADP+
-
 r
1.1.1.272 an (R)-2-hydroxycarboxylate + NADP+
-
 Haloferax mediterranei a 2-oxocarboxylate + NADPH + H+
-
 r
1.1.1.272 an (R)-2-hydroxycarboxylate + NADP+
-
 Haloferax mediterranei ATCC 33500 a 2-oxocarboxylate + NADPH + H+
-
 r
1.1.1.272 an (R)-2-hydroxycarboxylate + NADP+
-
 Haloferax mediterranei NBRC 14739 a 2-oxocarboxylate + NADPH + H+
-
 r
1.1.1.272 an (R)-2-hydroxycarboxylate + NADP+
-
 Haloferax mediterranei JCM 8866 a 2-oxocarboxylate + NADPH + H+
-
 r
1.1.1.272 an (R)-2-hydroxycarboxylate + NADP+
-
 Haloferax mediterranei R-4 a 2-oxocarboxylate + NADPH + H+
-
 r
1.1.1.272 an (R)-2-hydroxycarboxylate + NADP+
-
 Haloferax mediterranei DSM 1411 a 2-oxocarboxylate + NADPH + H+
-
 r
1.1.1.272 an (R)-2-hydroxycarboxylate + NADP+
-
 Haloferax mediterranei NCIMB 2177 a 2-oxocarboxylate + NADPH + H+
-
 r
1.1.1.272 additional information the DDH enzyme is a 2-oxocarboxylic reductase with broad substrate specificity. It shows a marked preference for those having an unbranched chain of 4-5 carbon atoms, such as 2-oxoisoleucine. It exhibits dual cofactor specificity, yet shows better catalytic efficiency with NADPH Haloferax mediterranei ?
-
-
1.1.1.272 additional information the DDH enzyme is a 2-oxocarboxylic reductase with broad substrate specificity. It shows a marked preference for those having an unbranched chain of 4-5 carbon atoms, such as 2-oxoisoleucine. It exhibits dual cofactor specificity, yet shows better catalytic efficiency with NADPH Haloferax mediterranei ATCC 33500 ?
-
-
1.1.1.272 additional information the DDH enzyme is a 2-oxocarboxylic reductase with broad substrate specificity. It shows a marked preference for those having an unbranched chain of 4-5 carbon atoms, such as 2-oxoisoleucine. It exhibits dual cofactor specificity, yet shows better catalytic efficiency with NADPH Haloferax mediterranei NBRC 14739 ?
-
-
1.1.1.272 additional information the DDH enzyme is a 2-oxocarboxylic reductase with broad substrate specificity. It shows a marked preference for those having an unbranched chain of 4-5 carbon atoms, such as 2-oxoisoleucine. It exhibits dual cofactor specificity, yet shows better catalytic efficiency with NADPH Haloferax mediterranei JCM 8866 ?
-
-
1.1.1.272 additional information the DDH enzyme is a 2-oxocarboxylic reductase with broad substrate specificity. It shows a marked preference for those having an unbranched chain of 4-5 carbon atoms, such as 2-oxoisoleucine. It exhibits dual cofactor specificity, yet shows better catalytic efficiency with NADPH Haloferax mediterranei R-4 ?
-
-
1.1.1.272 additional information the DDH enzyme is a 2-oxocarboxylic reductase with broad substrate specificity. It shows a marked preference for those having an unbranched chain of 4-5 carbon atoms, such as 2-oxoisoleucine. It exhibits dual cofactor specificity, yet shows better catalytic efficiency with NADPH Haloferax mediterranei DSM 1411 ?
-
-
1.1.1.272 additional information the DDH enzyme is a 2-oxocarboxylic reductase with broad substrate specificity. It shows a marked preference for those having an unbranched chain of 4-5 carbon atoms, such as 2-oxoisoleucine. It exhibits dual cofactor specificity, yet shows better catalytic efficiency with NADPH Haloferax mediterranei NCIMB 2177 ?
-
-
1.1.1.272 pyruvate + NADPH + H+
-
 Haloferax mediterranei D-lactate + NADP+
-
 r
1.1.1.272 pyruvate + NADPH + H+
-
 Haloferax mediterranei ATCC 33500 D-lactate + NADP+
-
 r
1.1.1.272 pyruvate + NADPH + H+
-
 Haloferax mediterranei NBRC 14739 D-lactate + NADP+
-
 r
1.1.1.272 pyruvate + NADPH + H+
-
 Haloferax mediterranei JCM 8866 D-lactate + NADP+
-
 r
1.1.1.272 pyruvate + NADPH + H+
-
 Haloferax mediterranei R-4 D-lactate + NADP+
-
 r
1.1.1.272 pyruvate + NADPH + H+
-
 Haloferax mediterranei DSM 1411 D-lactate + NADP+
-
 r
1.1.1.272 pyruvate + NADPH + H+
-
 Haloferax mediterranei NCIMB 2177 D-lactate + NADP+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.272 2HADH
-
 Haloferax mediterranei
1.1.1.272 D-2-hydroxyacid dehydrogenase
-
 Haloferax mediterranei
1.1.1.272 D-isomer specific 2-hydroxyacid dehydrogenase
-
 Haloferax mediterranei
1.1.1.272 DDH
-
 Haloferax mediterranei
1.1.1.272 DDH_HALMT
-
 Haloferax mediterranei

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.272 additional information the enzyme prefers NADPH over NADH Haloferax mediterranei
1.1.1.272 NADP+
-
 Haloferax mediterranei
1.1.1.272 NADPH
-
 Haloferax mediterranei

General Information

EC Number General Information Comment Organism
1.1.1.272 evolution the enzyme belongs to the family of D-isomer specific 2-hydroxyacid dehydrogenases (2HADHs) that contains a wide range of oxidoreductases with various metabolic roles as well as biotechnological applications. The family comprises 22 subfamilies, the enzyme from Haloferax mediterranei belongs to the DDH subfamily, phylogenetic analysis and tree, overview Haloferax mediterranei
1.1.1.272 additional information sequence-structure-function relationships, overview Haloferax mediterranei