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Literature summary extracted from

  • Tamaru, Y.; Umezawa, K.; Yoshida, M.
    Characterization of an aryl-alcohol oxidase from the plant saprophytic basidiomycete Coprinopsis cinerea with broad substrate specificity against aromatic alcohols (2018), Biotechnol. Lett., 40, 1077-1086 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.7 expression in Pichia pastoris Coprinopsis cinerea

Protein Variants

EC Number Protein Variants Comment Organism
1.1.3.7 L416F mutation decreases the activity with aromatic alcohols but maintains the activity with trans,trans-2,4-hexadien-1-ol Coprinopsis cinerea
1.1.3.7 L416W mutation decreases the activity with aromatic alcohols but maintains the activity with trans,trans-2,4-hexadien-1-ol Coprinopsis cinerea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.7 0.0012
-
 benzyl alcohol wild-type, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 0.004
-
 3-anisyl alcohol wild-type, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 0.0057
-
 3-Hydroxybenzyl alcohol wild-type, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 0.0059
-
 benzyl alcohol mutant L416W, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 0.0063
-
 Vanillyl alcohol wild-type, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 0.007
-
 (2E,4E)-hexa-2,4-dien-1-ol mutant L416W, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 0.0076
-
 4-anisyl alcohol mutant L416W, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 0.0079
-
 benzyl alcohol mutant L416F, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 0.012
-
 4-anisyl alcohol wild-type, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 0.0156
-
 (2E,4E)-hexa-2,4-dien-1-ol wild-type, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 0.0164
-
 (2E,4E)-hexa-2,4-dien-1-ol mutant L416F, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 0.019
-
 4-anisyl alcohol mutant L416F, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 0.031
-
 3-anisyl alcohol mutant L416W, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 0.034
-
 3-anisyl alcohol mutant L416F, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 0.042
-
 isovanillyl alcohol wild-type, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 0.0483
-
 veratryl alcohol wild-type, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 0.0563
-
 4-Hydroxybenzyl alcohol wild-type, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 0.154
-
 isovanillyl alcohol mutant L416W, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 0.163
-
 4-Hydroxybenzyl alcohol mutant L416F, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 0.279
-
 3-Hydroxybenzyl alcohol mutant L416W, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 0.301
-
 3-Hydroxybenzyl alcohol mutant L416F, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 0.318
-
 isovanillyl alcohol mutant L416F, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 0.419
-
 Vanillyl alcohol mutant L416W, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 0.424
-
 veratryl alcohol mutant L416F, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 0.466
-
 4-Hydroxybenzyl alcohol mutant L416W, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 0.686
-
 veratryl alcohol mutant L416W, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 0.98
-
 Vanillyl alcohol mutant L416F, pH 7, 25°C Coprinopsis cinerea

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.7 Coprinopsis cinerea A0A2Z5WP81
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.3.7 glycoprotein the amino acid sequence contains four and seven possible sites of N-glycosylation and O-glycosylation, respectively Coprinopsis cinerea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.7 (2E,4E)-hexa-2,4-dien-1-ol + O2 282% of the activity with benzyl alcohol Coprinopsis cinerea ? + H2O2
-
 ?
1.1.3.7 2,4-dimethoxybenzyl alcohol + O2 286% of the activity with benzyl alcohol Coprinopsis cinerea 2,4-dimethoxybenzyl aldehyde + H2O2
-
 ?
1.1.3.7 2-anisyl alcohol + O2 96% of the activity with benzyl alcohol Coprinopsis cinerea 2-anisyl aldehyde + H2O2
-
 ?
1.1.3.7 3-anisyl alcohol + O2 297% of the activity with benzyl alcohol Coprinopsis cinerea 3-anisyl aldehyde + H2O2
-
 ?
1.1.3.7 3-hydroxybenzyl alcohol + O2 221% of the activity with benzyl alcohol Coprinopsis cinerea 3-hydroxybenzyl aldehyde + H2O2
-
 ?
1.1.3.7 4-anisyl alcohol + O2 298% of the activity with benzyl alcohol Coprinopsis cinerea 4-anisyl aldehyde + H2O2
-
 ?
1.1.3.7 4-hydroxybenzyl alcohol + O2 207% of the activity with benzyl alcohol Coprinopsis cinerea 4-hydroxybenzyl aldehyde + H2O2
-
 ?
1.1.3.7 benzyl alcohol + O2
-
 Coprinopsis cinerea benzaldehyde + H2O2
-
 ?
1.1.3.7 benzyl alcohol + O2
-
 Coprinopsis cinerea benzyl aldehyde + H2O2
-
 ?
1.1.3.7 cinnamyl alcohol + O2 289% of the activity with benzyl alcohol Coprinopsis cinerea cinnamyl aldehyde + H2O2
-
 ?
1.1.3.7 isovanillyl alcohol + O2 73% of the activity with benzyl alcohol Coprinopsis cinerea isovanillyl aldehyde + H2O2
-
 ?
1.1.3.7 additional information no activity with mono- and disaccharides. No electron acceptor: DCIP Coprinopsis cinerea ?
-
-
1.1.3.7 vanillyl alcohol + O2
-
 Coprinopsis cinerea vanillyl aldehyde + H2O2
-
 ?
1.1.3.7 veratryl alcohol + O2 318% of the activity with benzyl alcohol Coprinopsis cinerea veratryl aldehyde + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.7 ? x * 62600, calculated from sequence, x * 65600, SDS-PAGE of endoH-treated protein Coprinopsis cinerea

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.3.7 0.54
-
 4-Hydroxybenzyl alcohol mutant L416W, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 0.81
-
 Vanillyl alcohol mutant L416W, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 1.17
-
 3-Hydroxybenzyl alcohol mutant L416W, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 2.05
-
 isovanillyl alcohol mutant L416W, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 2.96
-
 veratryl alcohol mutant L416W, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 3.49
-
 4-anisyl alcohol mutant L416W, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 3.84
-
 benzyl alcohol mutant L416W, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 4.81
-
 4-Hydroxybenzyl alcohol wild-type, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 6.13
-
 benzyl alcohol wild-type, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 7.02
-
 isovanillyl alcohol wild-type, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 7.5
-
 3-Hydroxybenzyl alcohol mutant L416F, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 7.66
-
 3-anisyl alcohol wild-type, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 8.03
-
 benzyl alcohol mutant L416F, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 8.67
-
 isovanillyl alcohol mutant L416F, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 8.77
-
 4-Hydroxybenzyl alcohol mutant L416F, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 9.91
-
 3-Hydroxybenzyl alcohol wild-type, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 11.3
-
 3-anisyl alcohol mutant L416W, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 12.4
-
 veratryl alcohol mutant L416F, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 12.4
-
 3-anisyl alcohol mutant L416F, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 12.5
-
 4-anisyl alcohol wild-type, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 13.2
-
 veratryl alcohol wild-type, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 14.2
-
 4-anisyl alcohol mutant L416F, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 14.7
-
 Vanillyl alcohol wild-type, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 17.3
-
 (2E,4E)-hexa-2,4-dien-1-ol mutant L416W, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 21.3
-
 Vanillyl alcohol mutant L416F, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 48.3
-
 (2E,4E)-hexa-2,4-dien-1-ol wild-type, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 68.4
-
 (2E,4E)-hexa-2,4-dien-1-ol mutant L416F, pH 7, 25°C Coprinopsis cinerea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.7 7
-
 assay at Coprinopsis cinerea

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.3.7 6 8
-
 Coprinopsis cinerea

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.7 FAD
-
 Coprinopsis cinerea

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.3.7 Coprinopsis cinerea calculated from sequence
-
 6.7

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.3.7 1.2
-
 4-Hydroxybenzyl alcohol mutant L416W, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 1.9
-
 Vanillyl alcohol mutant L416W, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 4.2
-
 3-Hydroxybenzyl alcohol mutant L416W, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 4.3
-
 veratryl alcohol mutant L416W, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 13.3
-
 isovanillyl alcohol mutant L416W, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 21.8
-
 Vanillyl alcohol mutant L416F, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 25
-
 3-Hydroxybenzyl alcohol mutant L416F, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 27.3
-
 isovanillyl alcohol mutant L416F, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 29.3
-
 veratryl alcohol mutant L416F, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 53.9
-
 4-Hydroxybenzyl alcohol mutant L416F, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 85.5
-
 4-Hydroxybenzyl alcohol wild-type, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 167
-
 isovanillyl alcohol wild-type, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 273
-
 veratryl alcohol wild-type, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 361
-
 3-anisyl alcohol mutant L416F, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 367
-
 3-anisyl alcohol mutant L416W, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 461
-
 4-anisyl alcohol mutant L416W, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 665
-
 benzyl alcohol mutant L416W, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 740
-
 4-anisyl alcohol mutant L416F, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 1020
-
 benzyl alcohol mutant L416F, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 1080
-
 4-anisyl alcohol wild-type, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 1750
-
 3-Hydroxybenzyl alcohol wild-type, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 1940
-
 3-anisyl alcohol wild-type, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 2350
-
 Vanillyl alcohol wild-type, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 2460
-
 (2E,4E)-hexa-2,4-dien-1-ol mutant L416W, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 3100
-
 (2E,4E)-hexa-2,4-dien-1-ol wild-type, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 4180
-
 (2E,4E)-hexa-2,4-dien-1-ol mutant L416F, pH 7, 25°C Coprinopsis cinerea
1.1.3.7 5060
-
 benzyl alcohol wild-type, pH 7, 25°C Coprinopsis cinerea