Literature summary extracted from

Fusco, G.; Goebel, G.; Zanoni, R.; Bracciale, M.P.; Favero, G.; Mazzei, F.; Lisdat, F.
Aqueous polythiophene electrosynthesis A new route to an efficient electrode coupling of PQQ-dependent glucose dehydrogenase for sensing and bioenergetic applications (2018), Biosens. Bioelectron., 112, 8-17 .

Application

EC Number	Application	Comment	Organism
1.1.99.35	biofuel production	the pyrroloquinoline quinone/glucose dehydrogenase (PQQ-GDH) electrode can be used as bioanode within a biofuel cell. PQQ-GDH is efficiently wired by the polymer polythiophene to the multiwalled carbon nanotubes (MWCNT) electrode	Acinetobacter calcoaceticus
1.1.99.35	diagnostics	biosensor	Acinetobacter calcoaceticus

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.1.99.35	0.74	-	D-glucose	pH and temperature not specified in the publication	Acinetobacter calcoaceticus

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
1.1.99.35	Ca2+	the holoenzyme is reconstituted by incubating the apoenzyme in 5 mM MES buffer at pH 6.5 with 1 mM CaCl2 and 0.060 mM pyrroloquinoline quinone for 3 h at room temperature in the dark	Acinetobacter calcoaceticus

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.1.99.35	Acinetobacter calcoaceticus	P05465	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.1.99.35	D-glucose + dimethyl((6-methyl-7-((4-nitrosophenyl)amino)-1H-imidazo[1,2-b]pyrazol-1-yl)methyl)phosphine oxide	-	Acinetobacter calcoaceticus	D-glucono-1,5-lactone + reduced dimethyl((6-methyl-7-((4-nitrosophenyl)amino)-1H-imidazo[1,2-b]pyrazol-1-yl)methyl)phosphine oxide	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
1.1.99.35	soluble PQQ-dependent glucose dehydrogenase	-	Acinetobacter calcoaceticus

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.1.99.35	pyrroloquinoline quinone	-	Acinetobacter calcoaceticus

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Release 2023.1 (January 2023)