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Literature summary extracted from

  • Kozono, I.; Hibi, M.; Takeuchi, M.; Ogawa, J.
    Purification and characterization of molybdenum-containing aldehyde dehydrogenase that oxidizes benzyl maltol derivative from Pseudomonas nitroreducens SB32154 (2020), Biosci. Biotechnol. Biochem., 84, 2390-2400 .
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.99.7 0.17
-
 2-nitrobenzaldehyde pH 6, 30°C Pseudomonas nitroreducens
1.2.99.7 0.22
-
 3-Nitrobenzaldehyde pH 6, 30°C Pseudomonas nitroreducens
1.2.99.7 0.35
-
 4-nitrobenzaldehyde pH 6, 30°C Pseudomonas nitroreducens
1.2.99.7 0.75
-
 4-hydroxybenzaldehyde pH 6, 30°C Pseudomonas nitroreducens
1.2.99.7 1
-
 2-Hydroxybenzaldehyde pH 6, 30°C Pseudomonas nitroreducens
1.2.99.7 1.1
-
 3-Hydroxybenzaldehyde pH 6, 30°C Pseudomonas nitroreducens
1.2.99.7 1.5
-
 cinnamaldehyde pH 6, 30°C Pseudomonas nitroreducens
1.2.99.7 7
-
 benzaldehyde pH 6, 30°C Pseudomonas nitroreducens
1.2.99.7 24
-
 3-(benzyloxy)-4-oxo-4H-pyran-2-carbaldehyde pH 6, 30°C Pseudomonas nitroreducens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.99.7 Molybdenum
-
 Pseudomonas nitroreducens

Organism

EC Number Organism UniProt Comment Textmining
1.2.99.7 Pseudomonas nitroreducens A0A5K7VLU0 and A0A5K7VFT8 A0A5K7VLU0 i.e. small subunit, A0A5K7VFT8 i.e. large subunit
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.99.7 2-hydroxybenzaldehyde + H2O + phenyazine methosulfate
-
 Pseudomonas nitroreducens 2-hydroxybenzylic acid + reduced phenyazine methosulfate
-
 ?
1.2.99.7 2-nitrobenzaldehyde + H2O + phenyazine methosulfate
-
 Pseudomonas nitroreducens 2-nitrobenzylic acid + reduced phenyazine methosulfate
-
 ?
1.2.99.7 3-(benzyloxy)-4-oxo-4H-pyran-2-carbaldehyde + H2O + phenyazine methosulfate
-
 Pseudomonas nitroreducens 3-(benzyloxy)-4-oxo-4H-pyran-2-carboxylic acid + reduced phenyazine methosulfate
-
 ?
1.2.99.7 3-hydroxybenzaldehyde + H2O + phenyazine methosulfate
-
 Pseudomonas nitroreducens 3-hydroxybenzylic acid + reduced phenyazine methosulfate
-
 ?
1.2.99.7 3-nitrobenzaldehyde + H2O + phenyazine methosulfate
-
 Pseudomonas nitroreducens 3-nitrobenzylic acid + reduced phenyazine methosulfate
-
 ?
1.2.99.7 4-hydroxybenzaldehyde + H2O + phenyazine methosulfate
-
 Pseudomonas nitroreducens 4-hydroxybenzylic acid + reduced phenyazine methosulfate
-
 ?
1.2.99.7 4-nitrobenzaldehyde + H2O + phenyazine methosulfate
-
 Pseudomonas nitroreducens 4-nitrobenzoic acid + reduced phenyazine methosulfate
-
 ?
1.2.99.7 benzaldehyde + H2O + phenyazine methosulfate
-
 Pseudomonas nitroreducens benzylic acid + reduced phenyazine methosulfate
-
 ?
1.2.99.7 cinnamaldehyde + H2O + phenyazine methosulfate
-
 Pseudomonas nitroreducens cinnamic acid + reduced phenyazine methosulfate
-
 ?
1.2.99.7 additional information additional albeit significantly less effective acdeptors are dichlorophenolindophenol, methylene blue, ferricyanide Pseudomonas nitroreducens ?
-
-

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.99.7 Fe-S center the small subunits contains a [2Fe-2S]-center Pseudomonas nitroreducens