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Literature summary extracted from

  • Akita, H.; Nakamichi, Y.; Morita, T.; Matsushika, A.
    Characterization of an NAD(P)+-dependent meso-diaminopimelate dehydrogenase from Thermosyntropha lipolytica (2020), Biochim. Biophys. Acta, 1868, 140476 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.1.16 expression in Escherichia coli Thermosyntropha lipolytica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.16 0.828
-
 NADP+ pH 10.5, 50°C Thermosyntropha lipolytica
1.4.1.16 5.68
-
 meso-2,6-diaminoheptanedioate pH 10.5, 50°C Thermosyntropha lipolytica
1.4.1.16 14.8
-
 NAD+ pH 10.5, 50°C Thermosyntropha lipolytica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.16 220000
-
 PAGE Thermosyntropha lipolytica

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.16 Thermosyntropha lipolytica A0A1M5M5H3
-
-
1.4.1.16 Thermosyntropha lipolytica DSM 11003 A0A1M5M5H3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.16 recombinant protein Thermosyntropha lipolytica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.16 31.8
-
 pH 10.5, 50°C Thermosyntropha lipolytica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.16 2-oxobutanoate + NH3 + NADPH + H+
-
 Thermosyntropha lipolytica D-2-aminobutanoate + NADP+
-
 ?
1.4.1.16 2-oxobutanoate + NH3 + NADPH + H+
-
 Thermosyntropha lipolytica DSM 11003 D-2-aminobutanoate + NADP+
-
 ?
1.4.1.16 2-oxoglutarate + NH3 + NADPH + H+
-
 Thermosyntropha lipolytica D-glutamate + NADP+
-
 ?
1.4.1.16 2-oxoglutarate + NH3 + NADPH + H+
-
 Thermosyntropha lipolytica DSM 11003 D-glutamate + NADP+
-
 ?
1.4.1.16 2-oxooctanoate + NH3 + NADPH + H+
-
 Thermosyntropha lipolytica D-2-aminooctanoate + NADP+
-
 ?
1.4.1.16 2-oxooctanoate + NH3 + NADPH + H+
-
 Thermosyntropha lipolytica DSM 11003 D-2-aminooctanoate + NADP+
-
 ?
1.4.1.16 3-methyl-2-oxobutanoate + NH3 + NADPH + H+
-
 Thermosyntropha lipolytica D-valine + NADP+
-
 ?
1.4.1.16 3-methyl-2-oxopentanoic acid + NH3 + NADPH + H+
-
 Thermosyntropha lipolytica D-isoleucine + NADP+
-
 ?
1.4.1.16 4-methyl-2-oxopentanoate + NH3 + NADPH + H+
-
 Thermosyntropha lipolytica D-leucine + NADP+
-
 ?
1.4.1.16 4-methylthio-2-oxobutanoate + NH3 + NADPH + H+
-
 Thermosyntropha lipolytica D-methionine + NADP+
-
 ?
1.4.1.16 meso-2,6-diaminoheptanedioate + H2O + NAD+
-
 Thermosyntropha lipolytica L-2-amino-6-oxoheptanedioate + NH3 + NADH + H+
-
 ?
1.4.1.16 meso-2,6-diaminoheptanedioate + H2O + NAD+
-
 Thermosyntropha lipolytica DSM 11003 L-2-amino-6-oxoheptanedioate + NH3 + NADH + H+
-
 ?
1.4.1.16 oxaloacetate + NH3 + NADPH + H+
-
 Thermosyntropha lipolytica D-aspartate + NADP+
-
 ?
1.4.1.16 pyruvate + NH3 + NADPH + H+
-
 Thermosyntropha lipolytica D-alanine + NADP+
-
 ?
1.4.1.16 pyruvate + NH3 + NADPH + H+
-
 Thermosyntropha lipolytica DSM 11003 D-alanine + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.1.16 hexamer 6 * 35000, SDS-PAGE Thermosyntropha lipolytica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.1.16 737
-
 meso-2,6-diaminoheptanedioate pH 10.5, 50°C Thermosyntropha lipolytica
1.4.1.16 775
-
 NAD+ pH 10.5, 50°C Thermosyntropha lipolytica
1.4.1.16 830
-
 NADP+ pH 10.5, 50°C Thermosyntropha lipolytica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.16 9
-
 optimum for reductive amination, 50°C Thermosyntropha lipolytica
1.4.1.16 10.5
-
 optimum for oxidative deamination, 50°C Thermosyntropha lipolytica

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.16 NAD+ NADP+ is 20fold preferred over NAD+ Thermosyntropha lipolytica
1.4.1.16 NADP+ NADP+ is 20fold preferred over NAD+ Thermosyntropha lipolytica

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.4.1.16 5.22
-
 NAD+ pH 10.5, 50°C Thermosyntropha lipolytica
1.4.1.16 13
-
 meso-2,6-diaminoheptanedioate pH 10.5, 50°C Thermosyntropha lipolytica
1.4.1.16 100.2
-
 NADP+ pH 10.5, 50°C Thermosyntropha lipolytica