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Literature summary extracted from

  • Round, J.; Roccor, R.; Li, S.N.; Eltis, L.D.
    A fatty acyl coenzyme A reductase promotes wax ester accumulation in Rhodococcus jostii RHA1 (2017), Appl. Environ. Microbiol., 83, e00902-17 .
    View publication on PubMedView publication on EuropePMC

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.84 Rhodococcus jostii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.84 Ni Sepharose 6 column chromatography Rhodococcus jostii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.84 0.0015
-
 with lauroyl-CoA as substrate, at pH 7.0 and 25°C Rhodococcus jostii
1.2.1.84 0.007
-
 with palmitoyl-CoA as substrate, at pH 7.0 and 25°C Rhodococcus jostii
1.2.1.84 0.041
-
 with oleoyl-CoA as substrate, at pH 7.0 and 25°C Rhodococcus jostii
1.2.1.84 0.045
-
 with stearoyl-CoA as substrate, at pH 7.0 and 25°C Rhodococcus jostii
1.2.1.84 2.8
-
 with cis-11-hexadecanal as substrate, at pH 7.0 and 25°C Rhodococcus jostii
1.2.1.84 3.3
-
 with decanal as substrate, at pH 7.0 and 25°C Rhodococcus jostii
1.2.1.84 5.3
-
 with dodecanal as substrate, at pH 7.0 and 25°C Rhodococcus jostii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.84 cis-11-hexadecenal + NADPH + H+
-
 Rhodococcus jostii cis-11-hexadecenol + NADP+
-
 ?
1.2.1.84 decanal + NADPH + H+
-
 Rhodococcus jostii decanol + NADP+
-
 ?
1.2.1.84 dodecanal + NADPH + H+
-
 Rhodococcus jostii dodecanol + NADP+
-
 ?
1.2.1.84 lauroyl-CoA + 2 NADPH + 2 H+
-
 Rhodococcus jostii dodecanol + CoA + 2 NADP+
-
 ?
1.2.1.84 additional information no activity is detected when NADPH is substituted with NADH Rhodococcus jostii ?
-
-
1.2.1.84 oleoyl-CoA + 2 NADPH + 2 H+
-
 Rhodococcus jostii octadecenol + CoA + 2 NADP+
-
 ?
1.2.1.84 palmitoyl-CoA + 2 NADPH + 2 H+
-
 Rhodococcus jostii hexadecanol + CoA + 2 NADP+
-
 ?
1.2.1.84 stearoyl-CoA + 2 NADPH + 2 H+
-
 Rhodococcus jostii stearyl alcohol + CoA + 2 NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.84 ? x * 73627, calculated from amino acid sequence Rhodococcus jostii
1.2.1.84 ? x * 75000, His6-tagged enzyme, SDS-PAGE Rhodococcus jostii

Synonyms

EC Number Synonyms Comment Organism
1.2.1.84 alcohol-forming fatty acyl-CoA reductase
-
 Rhodococcus jostii
1.2.1.84 fatty acyl coenzyme A reductase
-
 Rhodococcus jostii
1.2.1.84 FcrA
-
 Rhodococcus jostii
1.2.1.84 RS30405
-
 Rhodococcus jostii

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.84 NADPH
-
 Rhodococcus jostii

General Information

EC Number General Information Comment Organism
1.2.1.84 physiological function the enzyme promotes wax ester accumulation in Rhodococcus jostii RHA1 Rhodococcus jostii