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Literature summary extracted from

  • Gao, J.; Sun, Y.; Zhu, B.; Peng, R.; Wang, B.; Wang, L.; Li, Z.; Chen, L.; Yao, Q.
    Ectopic expression of sorbitol-6-phosphate 2-dehydrogenase gene from Haloarcula marismortui enhances salt tolerance in transgenic Arabidopsis thaliana (2018), Acta Physiol. Plant., 40, 108.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.140 expression in Pichia pastoris and Arabidopsis thaliana Haloarcula marismortui

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.140 NaCl activity is increased by nearly 120% when NaCl concentration is 2 M. Transgenic Arabidopsis thaliana ectopic expressing the enzyme (HmS6PDH) accumulates more sorbitol under salt stress Haloarcula marismortui

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.140 41000
-
 SDS-PAGE Haloarcula marismortui

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.140 D-fructose 6-phosphate + NADH + H+ Haloarcula marismortui
-
 D-sorbitol 6-phosphate + NAD+
-
 ?
1.1.1.140 D-fructose 6-phosphate + NADH + H+ Haloarcula marismortui ATCC 43049
-
 D-sorbitol 6-phosphate + NAD+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.140 Haloarcula marismortui AAV45891.1
-
-
1.1.1.140 Haloarcula marismortui ATCC 43049 AAV45891.1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.140
-
 Haloarcula marismortui

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.140 D-fructose 6-phosphate + NADH + H+
-
 Haloarcula marismortui D-sorbitol 6-phosphate + NAD+
-
 ?
1.1.1.140 D-fructose 6-phosphate + NADH + H+ the recombinant enzyme (HmS6PDH) has no activity to glucose or sorbitol Haloarcula marismortui D-sorbitol 6-phosphate + NAD+
-
 ?
1.1.1.140 D-fructose 6-phosphate + NADH + H+
-
 Haloarcula marismortui ATCC 43049 D-sorbitol 6-phosphate + NAD+
-
 ?
1.1.1.140 D-fructose 6-phosphate + NADH + H+ the recombinant enzyme (HmS6PDH) has no activity to glucose or sorbitol Haloarcula marismortui ATCC 43049 D-sorbitol 6-phosphate + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.140 ? x * 41000, SDS-PAGE Haloarcula marismortui

Synonyms

EC Number Synonyms Comment Organism
1.1.1.140 D-sorbitol-6-phosphate 2-dehydrogenase
-
 Haloarcula marismortui
1.1.1.140 HmSorD
-
 Haloarcula marismortui
1.1.1.140 S6PDH
-
 Haloarcula marismortui

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.140 60
-
-
 Haloarcula marismortui

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.140 50 70 50°C: about 50% of maximal activity, 70°C: about 30% of maximal activity Haloarcula marismortui

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.140 4
-
-
 Haloarcula marismortui

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.140 2.5 5.5 pH 2.5: about 40% of maximal activity, pH 5.5: about 45% of maximal activity Haloarcula marismortui