Any feedback?
Literature summary extracted from
- Structural and functional basis of difructose anhydride III hydrolase, which sequentially converts inulin using the same catalytic residue (2018), ACS Catal., 8, 10683-10697 .
No PubMed abstract available
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.1.178 | expressed in Escherichia coli BL21(DE3) cells | Pseudarthrobacter chlorophenolicus |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 4.2.1.178 | hanging drop vapor diffusion method, using 0.1 M sodium malonate (pH 4.2) and 10% (w/v) PEG 3350 | Pseudarthrobacter chlorophenolicus |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 4.2.1.178 | C387A | the mutant shows about 1.85fold increased activity with D-fructofuranose 2',1:2,3'-dianhydride compared to the wild type enzyme | Pseudarthrobacter chlorophenolicus |
| 4.2.1.178 | C387A/E210A | inactive | Pseudarthrobacter chlorophenolicus |
| 4.2.1.178 | D177A | inactive | Pseudarthrobacter chlorophenolicus |
| 4.2.1.178 | D177N | inactive | Pseudarthrobacter chlorophenolicus |
| 4.2.1.178 | D199A | inactive | Pseudarthrobacter chlorophenolicus |
| 4.2.1.178 | D199N | inactive | Pseudarthrobacter chlorophenolicus |
| 4.2.1.178 | E210A | inactive | Pseudarthrobacter chlorophenolicus |
| 4.2.1.178 | E210Q | inactive | Pseudarthrobacter chlorophenolicus |
| 4.2.1.178 | E307A | inactive | Pseudarthrobacter chlorophenolicus |
| 4.2.1.178 | F207A | the mutant shows 67.72% activity with D-fructofuranose 2',1:2,3'-dianhydride compared to the wild type enzyme | Pseudarthrobacter chlorophenolicus |
| 4.2.1.178 | F256A | the mutant shows 17.65% activity with D-fructofuranose 2',1:2,3'-dianhydride compared to the wild type enzyme | Pseudarthrobacter chlorophenolicus |
| 4.2.1.178 | F80A | the mutant shows 8.61% activity with D-fructofuranose 2',1:2,3'-dianhydride compared to the wild type enzyme | Pseudarthrobacter chlorophenolicus |
| 4.2.1.178 | I85A | the mutant shows 14.75% activity with D-fructofuranose 2',1:2,3'-dianhydride compared to the wild type enzyme | Pseudarthrobacter chlorophenolicus |
| 4.2.1.178 | Q222A | inactive | Pseudarthrobacter chlorophenolicus |
| 4.2.1.178 | Q391A | inactive | Pseudarthrobacter chlorophenolicus |
| 4.2.1.178 | R134A | inactive | Pseudarthrobacter chlorophenolicus |
| 4.2.1.178 | R258A | inactive | Pseudarthrobacter chlorophenolicus |
| 4.2.1.178 | W309F | the mutant shows 10.32% activity with D-fructofuranose 2',1:2,3'-dianhydride compared to the wild type enzyme | Pseudarthrobacter chlorophenolicus |
| 4.2.1.178 | Y163A | the mutant shows 8.09% activity with D-fructofuranose 2',1:2,3'-dianhydride compared to the wild type enzyme | Pseudarthrobacter chlorophenolicus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.178 | 98.06 | - |
alpha-D-fructofuranose-beta-D-fructofuranose 2',1:2,3'-dianhydride | mutant enzyme C387A, at pH 6.5 and 55°C | Pseudarthrobacter chlorophenolicus |  |
| 4.2.1.178 | 121.3 | - |
alpha-D-fructofuranose-beta-D-fructofuranose 2',1:2,3'-dianhydride | wild type enzyme, at pH 6.5 and 55°C | Pseudarthrobacter chlorophenolicus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.178 | 139000 | - |
gel filtration | Pseudarthrobacter chlorophenolicus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.178 | alpha-D-fructofuranose-beta-D-fructofuranose 2',1:2,3'-dianhydride + H2O | Pseudarthrobacter chlorophenolicus | - |
inulobiose | - |
r | |
| 4.2.1.178 | inulobiose | Pseudarthrobacter chlorophenolicus | - |
alpha-D-fructofuranose-beta-D-fructofuranose 2',1:2,3'-dianhydride + H2O | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.178 | Pseudarthrobacter chlorophenolicus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.1.178 | HiTrap Q Sepharose column chromatography and Superdex 200 gel filtration | Pseudarthrobacter chlorophenolicus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.178 | 5.17 | - |
mutant enzyme W309F, with alpha-D-fructofuranose-beta-D-fructofuranose 2',1:2,3'-dianhydride as substrate, at pH 6.5 and 55°C | Pseudarthrobacter chlorophenolicus |
| 4.2.1.178 | 8.19 | - |
mutant enzyme Y163A, with alpha-D-fructofuranose-beta-D-fructofuranose 2',1:2,3'-dianhydride as substrate, at pH 6.5 and 55°C | Pseudarthrobacter chlorophenolicus |
| 4.2.1.178 | 8.71 | - |
mutant enzyme F80A, with alpha-D-fructofuranose-beta-D-fructofuranose 2',1:2,3'-dianhydride as substrate, at pH 6.5 and 55°C | Pseudarthrobacter chlorophenolicus |
| 4.2.1.178 | 10.45 | - |
mutant enzyme S84A, with alpha-D-fructofuranose-beta-D-fructofuranose 2',1:2,3'-dianhydride as substrate, at pH 6.5 and 55°C | Pseudarthrobacter chlorophenolicus |
| 4.2.1.178 | 14.93 | - |
mutant enzyme I85A, with alpha-D-fructofuranose-beta-D-fructofuranose 2',1:2,3'-dianhydride as substrate, at pH 6.5 and 55°C | Pseudarthrobacter chlorophenolicus |
| 4.2.1.178 | 17.87 | - |
mutant enzyme F256A, with alpha-D-fructofuranose-beta-D-fructofuranose 2',1:2,3'-dianhydride as substrate, at pH 6.5 and 55°C | Pseudarthrobacter chlorophenolicus |
| 4.2.1.178 | 67.72 | - |
mutant enzyme F207A, with alpha-D-fructofuranose-beta-D-fructofuranose 2',1:2,3'-dianhydride as substrate, at pH 6.5 and 55°C | Pseudarthrobacter chlorophenolicus |
| 4.2.1.178 | 101.25 | - |
wild type enzyme with alpha-D-fructofuranose-beta-D-fructofuranose 2',1:2,3'-dianhydride as substrate, at pH 6.5 and 55°C | Pseudarthrobacter chlorophenolicus |
| 4.2.1.178 | 187.51 | - |
mutant enzyme C387A, with alpha-D-fructofuranose-beta-D-fructofuranose 2',1:2,3'-dianhydride as substrate, at pH 6.5 and 55°C | Pseudarthrobacter chlorophenolicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.178 | alpha-D-fructofuranose-beta-D-fructofuranose 2',1:2,3'-dianhydride + H2O | - |
Pseudarthrobacter chlorophenolicus | inulobiose | - |
r | |
| 4.2.1.178 | inulobiose | - |
Pseudarthrobacter chlorophenolicus | alpha-D-fructofuranose-beta-D-fructofuranose 2',1:2,3'-dianhydride + H2O | - |
r | |
| 4.2.1.178 | additional information | the enzyme is also capable of converting inulin to alpha-D-fructofuranose-beta-D-fructofuranose 2',1:2,3'-dianhydride (inulin fructotransferase activity) | Pseudarthrobacter chlorophenolicus | ? | - |
- |
|
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.1.178 | homotrimer | 3 * 47000, SDS-PAGE | Pseudarthrobacter chlorophenolicus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.2.1.178 | DFA-IIIase | - |
Pseudarthrobacter chlorophenolicus |
| 4.2.1.178 | difructose anhydride III hydrolase | - |
Pseudarthrobacter chlorophenolicus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.178 | 55 | - |
- |
Pseudarthrobacter chlorophenolicus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.178 | 30 | 80 | about 40% activity at 30°C, about 60% activity at 40°C, about 95% activity at 50°C, 100% activity at 55°C, about 95% activity at 60°C, about 50% activity at 65°C, about 40% activity at 70°C, about 30% activity at 80°C | Pseudarthrobacter chlorophenolicus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.178 | 6.5 | - |
- |
Pseudarthrobacter chlorophenolicus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.178 | 5.5 | 7 | more than 70% activity between pH 5.5 and 7.0 | Pseudarthrobacter chlorophenolicus |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.178 | 4.61 | - |
alpha-D-fructofuranose-beta-D-fructofuranose 2',1:2,3'-dianhydride | wild type enzyme, at pH 6.5 and 55°C | Pseudarthrobacter chlorophenolicus | |
| 4.2.1.178 | 8.59 | - |
alpha-D-fructofuranose-beta-D-fructofuranose 2',1:2,3'-dianhydride | mutant enzyme C387A, at pH 6.5 and 55°C | Pseudarthrobacter chlorophenolicus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
