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Literature summary extracted from

  • Smolkova, K.; Spackova, J.; Gotvaldova, K.; Dvorak, A.; Krenkova, A.; Hubalek, M.; Holendova, B.; Vitek, L.; Jezek, P.
    SIRT3 and GCN5L regulation of NADP+- and NADPH-driven reactions of mitochondrial isocitrate dehydrogenase IDH2 (2020), Sci. Rep., 10, 8677 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.42 K413Q the acetylation surrogate mutant of isoform IDH2 exhibits lower oxidative reaction rates than wild type. 2-Hydroxyglutarate production by the mutant is largely diminished at the enzymatic and cellular level Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.42 0.003
-
 isocitrate mutant enzyme K413Q, at pH 8.0 and 37°C Homo sapiens
1.1.1.42 0.005
-
 isocitrate wild type enzyme, at pH 8.0 and 37°C Homo sapiens
1.1.1.42 0.0176
-
 NADP+ wild type enzyme, at pH 8.0 and 37°C Homo sapiens
1.1.1.42 0.0569
-
 NADP+ mutant enzyme K413Q, at pH 8.0 and 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.42 mitochondrion
-
 Homo sapiens 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.42 Mg2+ 200 mM used in assay conditions Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.42 2-oxoglutarate + CO2 + NADPH + H+ Homo sapiens
-
 isocitrate + NADP+
-
 r
1.1.1.42 isocitrate + NADP+ Homo sapiens
-
 2-oxoglutarate + CO2 + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.42 Homo sapiens P48735
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.1.42 acetylation SIRT3 deacetylates isoform IDH2 Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.42 Flag-tag affinity column chromatography, and Superdex 200 gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.42 2-oxoglutarate + CO2 + NADPH + H+
-
 Homo sapiens isocitrate + NADP+
-
 r
1.1.1.42 isocitrate + NADP+
-
 Homo sapiens 2-oxoglutarate + CO2 + NADPH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.42 ? x * 45000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.1.1.42 IDH2
-
 Homo sapiens
1.1.1.42 isocitrate dehydrogenase 2
-
 Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.42 36.5
-
 melting temperature Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.42 4.9
-
 isocitrate mutant enzyme K413Q, at pH 8.0 and 37°C Homo sapiens
1.1.1.42 7.9
-
 NADP+ mutant enzyme K413Q, at pH 8.0 and 37°C Homo sapiens
1.1.1.42 15.5
-
 isocitrate wild type enzyme, at pH 8.0 and 37°C Homo sapiens
1.1.1.42 23.5
-
 NADP+ wild type enzyme, at pH 8.0 and 37°C Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.42 NADP+
-
 Homo sapiens
1.1.1.42 NADPH
-
 Homo sapiens