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Literature summary extracted from

  • Rudakova, N.; Alekseeva, M.; Zakharevich, N.; Mavletova, D.; Danilenko, V.
    Aminoglycoside phosphotransferase AphSR2 from Streptomyces rimosus ATCC 10970 dependence of antibiotic Resistance on serine-threonine protein kinases PkSR1 and PkSR2 (2020), Russ. J. Genet., 56, 112-117 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.190 gene aphSR2, sequence comparisons, recombinant expression in Escherichia coli strain BL21(DE3), coexpression of aphSR2 gene and genes pkSR1 and pkSR2, encoding serine-threonine protein kinases and localized in one cluster of the Streptomyces rimosus strain ATCC 10970 genome, causing a 2fold increase in resistance to neomycin Streptomyces rimosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.190 GTP + amikacin Streptomyces rimosus
-
 GDP + amikacin 2''-phosphate
-
 ?
2.7.1.190 GTP + amikacin Streptomyces rimosus ATCC 10970
-
 GDP + amikacin 2''-phosphate
-
 ?
2.7.1.190 GTP + gentamicin Streptomyces rimosus
-
 GDP + gentamicin 2''-phosphate
-
 ?
2.7.1.190 GTP + gentamicin Streptomyces rimosus ATCC 10970
-
 GDP + gentamicin 2''-phosphate
-
 ?
2.7.1.190 GTP + hygromycin Streptomyces rimosus
-
 GDP + hygromycin 2''-phosphate
-
 ?
2.7.1.190 GTP + hygromycin Streptomyces rimosus ATCC 10970
-
 GDP + hygromycin 2''-phosphate
-
 ?
2.7.1.190 GTP + isepamycin Streptomyces rimosus
-
 GDP + isepamycin 2''-phosphate
-
 ?
2.7.1.190 GTP + kanamycin Streptomyces rimosus
-
 GDP + kanamycin 2''-phosphate
-
 ?
2.7.1.190 GTP + kanamycin Streptomyces rimosus ATCC 10970
-
 GDP + kanamycin 2''-phosphate
-
 ?
2.7.1.190 GTP + neomycin Streptomyces rimosus
-
 GDP + neomycin 2''-phosphate
-
 ?
2.7.1.190 GTP + netilmycin Streptomyces rimosus
-
 GDP + netilmycin 2''-phosphate
-
 ?
2.7.1.190 GTP + sisomycin Streptomyces rimosus
-
 GDP + sisomycin 2''-phosphate
-
 ?
2.7.1.190 GTP + streptomycin Streptomyces rimosus
-
 GDP + streptomycin 2''-phosphate
-
 ?
2.7.1.190 GTP + tobramycin Streptomyces rimosus
-
 GDP + tobramycin 2''-phosphate
-
 ?
2.7.1.190 GTP + tobramycin Streptomyces rimosus ATCC 10970
-
 GDP + tobramycin 2''-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.190 Streptomyces rimosus
-
-
-
2.7.1.190 Streptomyces rimosus ATCC 10970
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.190 GTP + amikacin
-
 Streptomyces rimosus GDP + amikacin 2''-phosphate
-
 ?
2.7.1.190 GTP + amikacin
-
 Streptomyces rimosus ATCC 10970 GDP + amikacin 2''-phosphate
-
 ?
2.7.1.190 GTP + gentamicin
-
 Streptomyces rimosus GDP + gentamicin 2''-phosphate
-
 ?
2.7.1.190 GTP + gentamicin
-
 Streptomyces rimosus ATCC 10970 GDP + gentamicin 2''-phosphate
-
 ?
2.7.1.190 GTP + hygromycin
-
 Streptomyces rimosus GDP + hygromycin 2''-phosphate
-
 ?
2.7.1.190 GTP + hygromycin
-
 Streptomyces rimosus ATCC 10970 GDP + hygromycin 2''-phosphate
-
 ?
2.7.1.190 GTP + isepamycin
-
 Streptomyces rimosus GDP + isepamycin 2''-phosphate
-
 ?
2.7.1.190 GTP + kanamycin
-
 Streptomyces rimosus GDP + kanamycin 2''-phosphate
-
 ?
2.7.1.190 GTP + kanamycin
-
 Streptomyces rimosus ATCC 10970 GDP + kanamycin 2''-phosphate
-
 ?
2.7.1.190 GTP + neomycin
-
 Streptomyces rimosus GDP + neomycin 2''-phosphate
-
 ?
2.7.1.190 GTP + netilmycin
-
 Streptomyces rimosus GDP + netilmycin 2''-phosphate
-
 ?
2.7.1.190 GTP + sisomycin
-
 Streptomyces rimosus GDP + sisomycin 2''-phosphate
-
 ?
2.7.1.190 GTP + streptomycin
-
 Streptomyces rimosus GDP + streptomycin 2''-phosphate
-
 ?
2.7.1.190 GTP + tobramycin
-
 Streptomyces rimosus GDP + tobramycin 2''-phosphate
-
 ?
2.7.1.190 GTP + tobramycin
-
 Streptomyces rimosus ATCC 10970 GDP + tobramycin 2''-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.190 ? x * 41000, recombinant enzyme, SDS-PAGE Streptomyces rimosus

Synonyms

EC Number Synonyms Comment Organism
2.7.1.190 aminoglycoside phosphotransferase
-
 Streptomyces rimosus
2.7.1.190 AphSR2
-
 Streptomyces rimosus

General Information

EC Number General Information Comment Organism
2.7.1.190 evolution Streptomyces rimosus ATCC 10970 contains 14 genes annotated as aminoglycoside phosphotransferases in its genome: aphSR1-aphSR14 Streptomyces rimosus
2.7.1.190 additional information coexpression of aphSR2 gene and genes pkSR1 and pkSR2, encoding serine-threonine protein kinases, causes a 2fold increase in resistance to neomycin Streptomyces rimosus
2.7.1.190 physiological function change in the level of resistance to aminoglycoside antibiotics upon combined expression of the aphSR2, pkSR1, and pkSR2 genes in Escherichia coli strain BL21(DE3), overview Streptomyces rimosus