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Literature summary extracted from

  • Paidimuddala, B.; Krishna Aradhyam, G.; Gummadi, S.
    A halotolerant aldose reductase from Debaryomyces nepalensis gene isolation, overexpression and biochemical characterization (2017), RSC Adv., 7, 20384-20393 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.21 expression in Escherichia coli Debaryomyces nepalensis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.21 homology modeling of structure, a uniform negative electrostatic potential exists over the protein surface Debaryomyces nepalensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.21 KCl 0.5 M, 76% residual activity, 1 M, 37% residual activity Debaryomyces nepalensis
1.1.1.21 NaCl 0.5 M, 73% residual activity, 1 M, 29% residual activity Debaryomyces nepalensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.21 0.13
-
 NADPH pH 7.0, 45°C Debaryomyces nepalensis
1.1.1.21 1.7
-
 3-Nitrobenzaldehyde pH 7.0, 45°C Debaryomyces nepalensis
1.1.1.21 2
-
 isophthalaldehyde pH 7.0, 45°C Debaryomyces nepalensis
1.1.1.21 3.3
-
 benzaldehyde pH 7.0, 45°C Debaryomyces nepalensis
1.1.1.21 4.5
-
 Pyridine-3-aldehyde pH 7.0, 45°C Debaryomyces nepalensis
1.1.1.21 70
-
 Glyoxal pH 7.0, 45°C Debaryomyces nepalensis
1.1.1.21 106
-
 D-xylose pH 7.0, 45°C Debaryomyces nepalensis
1.1.1.21 110
-
 D-arabinose pH 7.0, 45°C Debaryomyces nepalensis
1.1.1.21 377
-
 D-ribose pH 7.0, 45°C Debaryomyces nepalensis
1.1.1.21 572
-
 D-galactose pH 7.0, 45°C Debaryomyces nepalensis

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.21 Debaryomyces nepalensis A0A0M4HL56
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.21 80
-
 substrate D-xylose, pH 7.0, 45°C Debaryomyces nepalensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.21 3-nitrobenzaldehyde + NADPH + H+
-
 Debaryomyces nepalensis 3-nitrobenzyl alcohol + NADP+
-
 ?
1.1.1.21 benzaldehyde + NADPH + H+
-
 Debaryomyces nepalensis benzyl alcohol + NADP+
-
 ?
1.1.1.21 D-arabinose + NADPH + H+
-
 Debaryomyces nepalensis arabitol + NADP+
-
 ?
1.1.1.21 D-galactose + NADPH + H+
-
 Debaryomyces nepalensis D-galactitol + NADP+
-
 ?
1.1.1.21 D-ribose + NADPH + H+
-
 Debaryomyces nepalensis adonitol + NADP+
-
 ?
1.1.1.21 D-xylose + NADPH + H+
-
 Debaryomyces nepalensis D-xylitol + NADP+
-
 ?
1.1.1.21 glyoxal + NADPH + H+
-
 Debaryomyces nepalensis ?
-
 ?
1.1.1.21 isophthalaldehyde + NADPH + H+
-
 Debaryomyces nepalensis ?
-
 ?
1.1.1.21 additional information poor substrates: D-glucose, D-fructose. No substrate: D-mannose Debaryomyces nepalensis ?
-
-
1.1.1.21 pyridine-3-aldehyde + NADPH + H+
-
 Debaryomyces nepalensis 3-pyridinemethanol + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.21 ? x * 36700, calculated from sequence Debaryomyces nepalensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.21 45
-
-
 Debaryomyces nepalensis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.21 50
-
 drastic loss of activity above Debaryomyces nepalensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.21 2 8 isophthalaldehyde pH 7.0, 45°C Debaryomyces nepalensis
1.1.1.21 7
-
 Pyridine-3-aldehyde pH 7.0, 45°C Debaryomyces nepalensis
1.1.1.21 11
-
 benzaldehyde pH 7.0, 45°C Debaryomyces nepalensis
1.1.1.21 40
-
 3-Nitrobenzaldehyde pH 7.0, 45°C Debaryomyces nepalensis
1.1.1.21 41
-
 Glyoxal pH 7.0, 45°C Debaryomyces nepalensis
1.1.1.21 92
-
 D-ribose pH 7.0, 45°C Debaryomyces nepalensis
1.1.1.21 106
-
 D-galactose pH 7.0, 45°C Debaryomyces nepalensis
1.1.1.21 108
-
 D-xylose pH 7.0, 45°C Debaryomyces nepalensis
1.1.1.21 163
-
 D-arabinose pH 7.0, 45°C Debaryomyces nepalensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.21 7
-
-
 Debaryomyces nepalensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.21 6
-
 60% of maximum activity Debaryomyces nepalensis
1.1.1.21 8
-
 55 of maximum activity Debaryomyces nepalensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.21 NADPH shows strict NADPH dependence Debaryomyces nepalensis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.21 0.2
-
 D-galactose pH 7.0, 45°C Debaryomyces nepalensis
1.1.1.21 0.24
-
 D-ribose pH 7.0, 45°C Debaryomyces nepalensis
1.1.1.21 0.58
-
 Glyoxal pH 7.0, 45°C Debaryomyces nepalensis
1.1.1.21 1
-
 D-xylose pH 7.0, 45°C Debaryomyces nepalensis
1.1.1.21 1.5
-
 D-arabinose pH 7.0, 45°C Debaryomyces nepalensis
1.1.1.21 1.6
-
 Pyridine-3-aldehyde pH 7.0, 45°C Debaryomyces nepalensis
1.1.1.21 3.3
-
 benzaldehyde pH 7.0, 45°C Debaryomyces nepalensis
1.1.1.21 12
-
 isophthalaldehyde pH 7.0, 45°C Debaryomyces nepalensis
1.1.1.21 24
-
 3-Nitrobenzaldehyde pH 7.0, 45°C Debaryomyces nepalensis
1.1.1.21 728
-
 NADPH pH 7.0, 45°C Debaryomyces nepalensis