Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Franco-Echevarria, E.; Sanz-Aparicio, J.; Troffer-Charlier, N.; Poterszman, A.; Gonzalez, B.
    Crystallization and preliminary X-ray diffraction analysis of a mammal inositol 1,3,4,5,6-pentakisphosphate 2-kinase (2017), Protein J., 36, 240-248 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.158 gene ipk1, recombinant expression of LSLt-tagged enzyme in Escherichia coli strain BL21 Star (DE3), and recombinant expression of GST-tagged enzyme in Spodoptera frugiperda Sf21 cells. LSLt is a fragment of Laetiporus sulphurous lectin Mus musculus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.158 purified recombinant detagged enzyme, sitting drop vapour diffusion, mixing of 250 nl of 5-6 mg/ml protein solution with 250 nl of crystallization solution containing 0.2 M MgCl2, 0.1 M MES, pH 6.25, and 18% v/v PEG 6000 for insect cell-expressed enzyme, and 0.2 M MgCl2, 0.1 M MES, pH 6.25, and 12% v/v PEG 6000 for bacteria-expressed enzyme, equilibration against 0.065 ml of crystallization solution as reservoir, method screening and optimization, X-ray diffraction structure determination and analysis at resolution of 4.3 A (Escherichia coli expression) and 4.0 A (insect cell expression), modeling Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.158 Mg2+ required Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.158 ATP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate Mus musculus
-
 ADP + 1D-myo-inositol hexakisphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.158 Mus musculus Q6P1C1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.158 recombinant LSLt-tagged enzyme from Escherichia coli strain BL21 Star (DE3) by heparin affinity chromatography and gel filtration followed by tag cleavage with TEV protease and again heparin affinity chromatography and gel filtration. The recombinant GST-tagged enzyme from Sf21 insect cells is purified by glutathione affinity chromatography and tag cleavage with PreScission protease, followed by heparin affinity chromatography and gel filtration Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.158 ATP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
 Mus musculus ADP + 1D-myo-inositol hexakisphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.158 monomer
-
 Mus musculus

Synonyms

EC Number Synonyms Comment Organism
2.7.1.158 inositol 1,3,4,5,6-pentakisphosphate 2-kinase
-
 Mus musculus
2.7.1.158 IP5 2-K
-
 Mus musculus
2.7.1.158 Ipk1
-
 Mus musculus
2.7.1.158 mIP5 2-K
-
 Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.158 ATP
-
 Mus musculus

General Information

EC Number General Information Comment Organism
2.7.1.158 physiological function inositol 1,3,4,5,6-pentakisphosphate 2-kinase (IP5 2-K) phosphorylates inositol pentakisphosphate (IP5) to produce inositol 1,2,3,4,5,6-hexakisphosphate (IP6), from ATP Mus musculus