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Literature summary extracted from

  • Balan, D.; Tokas, J.; Singal, H.R.
    UDP-glucose pyrophosphorylase Isolation, purification and characterization from developing thermotolerant wheat (Triticum aestivum) grains (2018), Protein Expr. Purif., 148, 68-77 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.9 ADP-glucose 2 mM, 2.1% stimulation Triticum aestivum
2.7.7.9 NADP+ 2 mM, 15% stimulation Triticum aestivum
2.7.7.9 UDP-glucose 2 mM, 18% stimulation, substrate activation Triticum aestivum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.9 ATP 2 mM, 9.3% inhibition Triticum aestivum
2.7.7.9 CTP 2 mM, 13% inhibition Triticum aestivum
2.7.7.9 D-glucose 1-phosphate 2 mM, 14% inhibition Triticum aestivum
2.7.7.9 D-glucose 6-phosphate 2 mM, 18% inhibition Triticum aestivum
2.7.7.9 GTP 2 mM, 15% inhibition Triticum aestivum
2.7.7.9 UTP 2 mM, 20% inhibition Triticum aestivum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.9 0.9
-
 alpha-D-glucose 1-phosphate pH 9.0, temperature not specified in the publication Triticum aestivum
2.7.7.9 1.66
-
 diphosphate pH 9.0, temperature not specified in the publication Triticum aestivum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.9 82000
-
 gel filtration Triticum aestivum

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.9 Triticum aestivum
-
 L. em Thell, four different thermotolerant varieties: WH-1021, PBW-373, Raj-3765 and DBW-16
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.9 enzyme from variety WH-1021 Triticum aestivum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.9 caryopsis the specific activity of UGPase is highest 21 days after anthesis in the variety WH-1021 Triticum aestivum
-

Storage Stability

EC Number Storage Stability Organism
2.7.7.9 0-4°C, stable for 10 days Triticum aestivum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.9 diphosphate + UDP-glucose
-
 Triticum aestivum UTP + alpha-D-glucose 1-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.9 homodimer 2 * 39000, SDS-PAGE Triticum aestivum

Synonyms

EC Number Synonyms Comment Organism
2.7.7.9 UDP-glucose pyrophosphorylase
-
 Triticum aestivum
2.7.7.9 UGPase
-
 Triticum aestivum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.9 35
-
-
 Triticum aestivum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.7.9 30 40 30°C: about 40% of maximal activity, 40°C: about 60% of maximal activity Triticum aestivum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.9 50
-
 1 h, stable up to 50°C Triticum aestivum
2.7.7.9 65
-
 1 90% loss of activity Triticum aestivum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.9 8.5
-
-
 Triticum aestivum