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Literature summary extracted from

  • Wang, X.; Inaoka, D.K.; Shiba, T.; Balogun, E.O.; Allmann, S.; Watanabe, Y.I.; Boshart, M.; Kita, K.; Harada, S.
    Expression, purification, and crystallization of type 1 isocitrate dehydrogenase from Trypanosoma brucei (2017), Protein Expr. Purif., 138, 56-62 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.41 expressed in Escherichia coli BL21star(DE3) cells Trypanosoma brucei brucei

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.41 sitting drop vapor diffusion method, using 100 mM HEPES-NaOH buffer, pH 7.2, 200 mM NaCl and 22% (w/v) PEG6000 Trypanosoma brucei brucei

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.41 peroxisome
-
 Trypanosoma brucei brucei 5777
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.41 Mn2+ 0.5 mM used in assay conditions Trypanosoma brucei brucei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.41 97500
-
 gel filtration Trypanosoma brucei brucei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.41 isocitrate + NAD+ Trypanosoma brucei brucei
-
 2-oxoglutarate + CO2 + NADH + H+
-
 ?
1.1.1.41 isocitrate + NAD+ Trypanosoma brucei brucei ILtat 1.4
-
 2-oxoglutarate + CO2 + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.41 Trypanosoma brucei brucei
-
-
-
1.1.1.41 Trypanosoma brucei brucei ILtat 1.4
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.41 Ni-NTA agarose column chromatography, and Superdex 200 gel filtration Trypanosoma brucei brucei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.41 14.7
-
 with NADP+ as cosubstrate, at pH 8.0, temperature not specified in the publication Trypanosoma brucei brucei
1.1.1.41 22.1
-
 with NAD+ as cosubstrate, at pH 8.0, temperature not specified in the publication Trypanosoma brucei brucei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.41 isocitrate + NAD+
-
 Trypanosoma brucei brucei 2-oxoglutarate + CO2 + NADH + H+
-
 ?
1.1.1.41 isocitrate + NAD+ the enzyme has considerably high activity with NAD+ compared with NADP+ as electron acceptor Trypanosoma brucei brucei 2-oxoglutarate + CO2 + NADH + H+
-
 ?
1.1.1.41 isocitrate + NAD+
-
 Trypanosoma brucei brucei ILtat 1.4 2-oxoglutarate + CO2 + NADH + H+
-
 ?
1.1.1.41 isocitrate + NAD+ the enzyme has considerably high activity with NAD+ compared with NADP+ as electron acceptor Trypanosoma brucei brucei ILtat 1.4 2-oxoglutarate + CO2 + NADH + H+
-
 ?
1.1.1.41 isocitrate + NADP+ the enzyme has considerably high activity with NAD+ compared with NADP+ as electron acceptor Trypanosoma brucei brucei 2-oxoglutarate + CO2 + NADPH + H+
-
 ?
1.1.1.41 isocitrate + NADP+ the enzyme has considerably high activity with NAD+ compared with NADP+ as electron acceptor Trypanosoma brucei brucei ILtat 1.4 2-oxoglutarate + CO2 + NADPH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.41 homodimer 2 * 60000, His6-SUMO-tagged enzyme, SDS-PAGE Trypanosoma brucei brucei

Synonyms

EC Number Synonyms Comment Organism
1.1.1.41 IDH1
-
 Trypanosoma brucei brucei
1.1.1.41 NAD+-dependent type 1 IDH
-
 Trypanosoma brucei brucei
1.1.1.41 type 1 isocitrate dehydrogenase
-
 Trypanosoma brucei brucei

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.41 NAD+
-
 Trypanosoma brucei brucei