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Literature summary extracted from
- Chromohalobacter salixigens uronate dehydrogenase Directed evolution for improved thermal stability and mutant CsUDH-inc X-ray crystal structure (2022), Process Biochem., 114, 185-192.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.1.203 | expressed in Escherichia coli BL21(DE3) cells | Chromohalobacter salexigens |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 1.1.1.203 | sitting drop vapor diffusion method, using 0.1 M MES pH6.5 and 30% (w/v) 2-methyl-2-4-pentanediol | Chromohalobacter salexigens |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.1.1.203 | A172V | the mutant shows reduced catalytic efficiency with beta-D-glucuronate and beta-D-galacturonate compared to the wild type enzyme | Chromohalobacter salexigens |
| 1.1.1.203 | D246N | the mutant shows reduced catalytic efficiency with beta-D-glucuronate and beta-D-galacturonate compared to the wild type enzyme | Chromohalobacter salexigens |
| 1.1.1.203 | I114T | the mutant shows reduced catalytic efficiency with beta-D-glucuronate and beta-D-galacturonate compared to the wild type enzyme | Chromohalobacter salexigens |
| 1.1.1.203 | I88L | the mutant shows reduced catalytic efficiency with beta-D-glucuronate and beta-D-galacturonate compared to the wild type enzyme | Chromohalobacter salexigens |
| 1.1.1.203 | L158A/N159C/I160V | the mutant shows increased catalytic efficiency with beta-D-glucuronate and beta-D-galacturonate compared to the wild type enzyme | Chromohalobacter salexigens |
| 1.1.1.203 | M175I | the mutant shows reduced catalytic efficiency with beta-D-glucuronate and beta-D-galacturonate compared to the wild type enzyme | Chromohalobacter salexigens |
| 1.1.1.203 | N99K/L100H/K102V/P103K/I105V | the mutant shows wild type activity with beta-D-glucuronate and reduced catalytic efficiency with beta-D-galacturonate compared to the wild type enzyme | Chromohalobacter salexigens |
| 1.1.1.203 | N99K/L100H/K102V/P103K/I105V/Y116F/T113V/A172V/M175I/D236N | the mutant shows reduced catalytic efficiency with beta-D-glucuronate and beta-D-galacturonate compared to the wild type enzyme | Chromohalobacter salexigens |
| 1.1.1.203 | Q55R | the mutant shows wild type activity with beta-D-glucuronate and reduced catalytic efficiency with beta-D-galacturonate compared to the wild type enzyme | Chromohalobacter salexigens |
| 1.1.1.203 | Q55R/I88L/N99K/L100H/K102V/P103K/I105V/T113V/I114T/Y116F/L158A/N159C/I160V/A172V/M175I/D246N | the mutant shows reduced catalytic efficiency with beta-D-glucuronate and beta-D-galacturonate compared to the wild type enzyme | Chromohalobacter salexigens |
| 1.1.1.203 | T113V | the mutant shows reduced catalytic efficiency with beta-D-glucuronate and beta-D-galacturonate compared to the wild type enzyme | Chromohalobacter salexigens |
| 1.1.1.203 | T113V/A172V/D246N | the mutant shows reduced catalytic efficiency with beta-D-glucuronate and beta-D-galacturonate compared to the wild type enzyme | Chromohalobacter salexigens |
| 1.1.1.203 | T113V/A172V/M175I | the mutant shows reduced catalytic efficiency with beta-D-glucuronate and beta-D-galacturonate compared to the wild type enzyme | Chromohalobacter salexigens |
| 1.1.1.203 | T113V/A172V/M175I/D236N | the mutant shows reduced catalytic efficiency with beta-D-glucuronate and beta-D-galacturonate compared to the wild type enzyme | Chromohalobacter salexigens |
| 1.1.1.203 | T113V/M175I/D246N | the mutant shows increased catalytic efficiency with beta-D-glucuronate and decreased catalytic efficiency with beta-D-galacturonate compared to the wild type enzyme | Chromohalobacter salexigens |
| 1.1.1.203 | Y116F | the mutant shows reduced catalytic efficiency with beta-D-glucuronate and beta-D-galacturonate compared to the wild type enzyme | Chromohalobacter salexigens |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.203 | 0.13 | - |
beta-D-galacturonate | mutant enzyme N99K/L100H/K102V/P103K/I105V, at pH 8.0 and 25°C | Chromohalobacter salexigens |  |
| 1.1.1.203 | 0.14 | - |
beta-D-galacturonate | mutant enzyme L158A/N159C/I160V, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 0.15 | - |
beta-D-galacturonate | mutant enzyme T113V, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 0.16 | - |
beta-D-galacturonate | mutant enzyme N99K/L100H/K102V/P103K/I105V/Y116F/T113V/A172V/M175I/D236N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 0.18 | - |
beta-D-galacturonate | wild type enzyme, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 0.18 | - |
beta-D-galacturonate | mutant enzyme A172V, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 0.18 | - |
beta-D-galacturonate | mutant enzyme D246N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 0.18 | - |
beta-D-galacturonate | mutant enzyme M175I, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 0.18 | - |
beta-D-galacturonate | mutant enzyme T113V/A172V/M175I, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 0.18 | - |
beta-D-galacturonate | mutant enzyme T113V/A172V/M175I/D236N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 0.19 | - |
beta-D-galacturonate | mutant enzyme Q55R, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 0.19 | - |
beta-D-galacturonate | mutant enzyme T113V/M175I/D246N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 0.2 | - |
beta-D-galacturonate | mutant enzyme I88L, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 0.21 | - |
beta-D-galacturonate | mutant enzyme Q55R/I88L/N99K/L100H/K102V/P103K/I105V/T113V/I114T/Y116F/L158A/N159C/I160V/A172V/M175I/D246N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 0.22 | - |
beta-D-galacturonate | mutant enzyme T113V/A172V/D246N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 0.22 | - |
beta-D-galacturonate | mutant enzyme Y116F, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 0.41 | - |
beta-D-galacturonate | mutant enzyme I114T, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 0.43 | - |
beta-D-glucuronate | mutant enzyme N99K/L100H/K102V/P103K/I105V/Y116F/T113V/A172V/M175I/D236N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 0.65 | - |
beta-D-glucuronate | mutant enzyme Q55R/I88L/N99K/L100H/K102V/P103K/I105V/T113V/I114T/Y116F/L158A/N159C/I160V/A172V/M175I/D246N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 0.9 | - |
beta-D-glucuronate | mutant enzyme N99K/L100H/K102V/P103K/I105V, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 0.95 | - |
beta-D-glucuronate | mutant enzyme T113V/A172V/M175I/D236N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 1.03 | - |
beta-D-glucuronate | mutant enzyme M175I, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 1.1 | - |
beta-D-glucuronate | mutant enzyme T113V/A172V/M175I, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 1.1 | - |
beta-D-glucuronate | mutant enzyme T113V/M175I/D246N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 1.2 | - |
beta-D-glucuronate | mutant enzyme L158A/N159C/I160V, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 1.3 | - |
beta-D-glucuronate | mutant enzyme D246N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 1.5 | - |
beta-D-glucuronate | mutant enzyme I88L, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 1.5 | - |
beta-D-glucuronate | mutant enzyme T113V/A172V/D246N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 1.6 | - |
beta-D-glucuronate | wild type enzyme, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 1.7 | - |
beta-D-glucuronate | mutant enzyme A172V, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 1.7 | - |
beta-D-glucuronate | mutant enzyme Q55R, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 1.7 | - |
beta-D-glucuronate | mutant enzyme T113V, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 2 | - |
beta-D-glucuronate | mutant enzyme Y116F, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 3.2 | - |
beta-D-glucuronate | mutant enzyme I114T, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.203 | beta-D-galacturonate + NAD+ | Chromohalobacter salexigens | - |
D-galactaro-1,5-lactone + NADH + H+ | - |
? | |
| 1.1.1.203 | beta-D-glucuronate + NAD+ | Chromohalobacter salexigens | - |
D-glucaro-1,5-lactone + NADH + H+ | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.203 | Chromohalobacter salexigens | Q1QUN7 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.203 | Ni-NTA column chromatography and Superdex 200 gel filtration | Chromohalobacter salexigens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.203 | beta-D-galacturonate + NAD+ | - |
Chromohalobacter salexigens | D-galactaro-1,5-lactone + NADH + H+ | - |
? | |
| 1.1.1.203 | beta-D-glucuronate + NAD+ | - |
Chromohalobacter salexigens | D-glucaro-1,5-lactone + NADH + H+ | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.1.203 | Udh | - |
Chromohalobacter salexigens |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.203 | 2 | 8 | beta-D-galacturonate | mutant enzyme Q55R, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 17 | - |
beta-D-galacturonate | mutant enzyme I114T, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 17 | - |
beta-D-galacturonate | mutant enzyme N99K/L100H/K102V/P103K/I105V, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 17 | - |
beta-D-galacturonate | mutant enzyme Q55R/I88L/N99K/L100H/K102V/P103K/I105V/T113V/I114T/Y116F/L158A/N159C/I160V/A172V/M175I/D246N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 19 | - |
beta-D-galacturonate | mutant enzyme T113V/A172V/M175I, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 20 | - |
beta-D-galacturonate | mutant enzyme M175I, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 20 | - |
beta-D-galacturonate | mutant enzyme N99K/L100H/K102V/P103K/I105V/Y116F/T113V/A172V/M175I/D236N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 20 | - |
beta-D-galacturonate | mutant enzyme T113V, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 22 | - |
beta-D-galacturonate | mutant enzyme D246N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 22 | - |
beta-D-galacturonate | mutant enzyme I88L, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 22 | - |
beta-D-galacturonate | mutant enzyme T113V/A172V/M175I/D236N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 25 | - |
beta-D-galacturonate | mutant enzyme Y116F, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 27 | - |
beta-D-galacturonate | mutant enzyme A172V, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 27 | - |
beta-D-galacturonate | mutant enzyme L158A/N159C/I160V, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 29 | - |
beta-D-galacturonate | mutant enzyme T113V/M175I/D246N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 30 | - |
beta-D-galacturonate | mutant enzyme T113V/A172V/D246N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 30.8 | - |
beta-D-galacturonate | wild type enzyme, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 50 | - |
beta-D-glucuronate | mutant enzyme Q55R/I88L/N99K/L100H/K102V/P103K/I105V/T113V/I114T/Y116F/L158A/N159C/I160V/A172V/M175I/D246N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 59 | - |
beta-D-glucuronate | mutant enzyme N99K/L100H/K102V/P103K/I105V/Y116F/T113V/A172V/M175I/D236N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 119 | - |
beta-D-glucuronate | mutant enzyme T113V/A172V/M175I, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 130 | - |
beta-D-glucuronate | mutant enzyme T113V/A172V/M175I/D236N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 142 | - |
beta-D-glucuronate | mutant enzyme N99K/L100H/K102V/P103K/I105V, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 153 | - |
beta-D-glucuronate | mutant enzyme M175I, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 155 | - |
beta-D-glucuronate | mutant enzyme I114T, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 179 | - |
beta-D-glucuronate | mutant enzyme T113V/M175I/D246N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 192 | - |
beta-D-glucuronate | mutant enzyme D246N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 192 | - |
beta-D-glucuronate | mutant enzyme I88L, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 192 | - |
beta-D-glucuronate | mutant enzyme T113V, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 200 | - |
beta-D-glucuronate | mutant enzyme Y116F, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 214 | - |
beta-D-glucuronate | mutant enzyme T113V/A172V/D246N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 219 | - |
beta-D-glucuronate | mutant enzyme L158A/N159C/I160V, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 225 | - |
beta-D-glucuronate | mutant enzyme A172V, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 263 | - |
beta-D-glucuronate | wild type enzyme, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 270 | - |
beta-D-glucuronate | mutant enzyme Q55R, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.203 | NAD+ | - |
Chromohalobacter salexigens | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.203 | 41 | - |
beta-D-galacturonate | mutant enzyme I114T, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 48 | - |
beta-D-glucuronate | mutant enzyme I114T, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 77 | - |
beta-D-glucuronate | mutant enzyme Q55R/I88L/N99K/L100H/K102V/P103K/I105V/T113V/I114T/Y116F/L158A/N159C/I160V/A172V/M175I/D246N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 78 | - |
beta-D-galacturonate | mutant enzyme Q55R/I88L/N99K/L100H/K102V/P103K/I105V/T113V/I114T/Y116F/L158A/N159C/I160V/A172V/M175I/D246N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 101 | - |
beta-D-glucuronate | mutant enzyme Y116F, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 104 | - |
beta-D-galacturonate | mutant enzyme T113V/A172V/M175I, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 111 | - |
beta-D-glucuronate | mutant enzyme T113V/A172V/M175I, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 111 | - |
beta-D-galacturonate | mutant enzyme Y116F, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 112 | - |
beta-D-galacturonate | mutant enzyme I88L, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 115 | - |
beta-D-glucuronate | mutant enzyme T113V, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 116 | - |
beta-D-galacturonate | mutant enzyme M175I, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 125 | - |
beta-D-glucuronate | mutant enzyme I88L, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 125 | - |
beta-D-galacturonate | mutant enzyme N99K/L100H/K102V/P103K/I105V, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 125 | - |
beta-D-galacturonate | mutant enzyme T113V/A172V/M175I/D236N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 126 | - |
beta-D-galacturonate | mutant enzyme D246N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 126 | - |
beta-D-galacturonate | mutant enzyme N99K/L100H/K102V/P103K/I105V/Y116F/T113V/A172V/M175I/D236N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 132 | - |
beta-D-galacturonate | mutant enzyme T113V, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 136 | - |
beta-D-glucuronate | mutant enzyme A172V, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 136 | - |
beta-D-glucuronate | mutant enzyme N99K/L100H/K102V/P103K/I105V/Y116F/T113V/A172V/M175I/D236N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 138 | - |
beta-D-galacturonate | mutant enzyme T113V/A172V/D246N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 138 | - |
beta-D-glucuronate | mutant enzyme T113V/A172V/M175I/D236N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 139 | - |
beta-D-glucuronate | mutant enzyme T113V/A172V/D246N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 146 | - |
beta-D-glucuronate | mutant enzyme D246N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 149 | - |
beta-D-glucuronate | mutant enzyme M175I, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 151 | - |
beta-D-galacturonate | mutant enzyme A172V, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 152 | - |
beta-D-galacturonate | mutant enzyme Q55R, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 155 | - |
beta-D-galacturonate | mutant enzyme T113V/M175I/D246N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 161 | - |
beta-D-glucuronate | wild type enzyme, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 161 | - |
beta-D-glucuronate | mutant enzyme Q55R, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 162 | - |
beta-D-glucuronate | mutant enzyme N99K/L100H/K102V/P103K/I105V, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 164 | - |
beta-D-glucuronate | mutant enzyme T113V/M175I/D246N, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 172 | - |
beta-D-galacturonate | wild type enzyme, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 186 | - |
beta-D-glucuronate | mutant enzyme L158A/N159C/I160V, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
| 1.1.1.203 | 187 | - |
beta-D-galacturonate | mutant enzyme L158A/N159C/I160V, at pH 8.0 and 25°C | Chromohalobacter salexigens | |
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