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Literature summary extracted from

  • Foulon, V.; Antonenkov, V.D.; Croes, K.; Waelkens, E.; Mannaerts, G.P.; Van Veldhoven, P.P.; Casteels, M.
    Purification, molecular cloning, and expression of 2-hydroxyphytanoyl-CoA lyase, a peroxisomal thiamine pyrophosphate-dependent enzyme that catalyzes the carbon-carbon bond cleavage during alpha-oxidation of 3-methyl-branched fatty acids (1999), Proc. Natl. Acad. Sci. USA, 96, 10039-10044 .
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.63 0.015
-
 2-hydroxy-3-methylhexadecanoyl-CoA at pH 7.5 and 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.2.63 peroxisome
-
 Rattus norvegicus 5777
-
4.1.2.63 peroxisome
-
 Homo sapiens 5777
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.2.63 Mg2+ optimum activity in the presence of 0.8 mM Mg2+ Rattus norvegicus
4.1.2.63 Mg2+ optimum activity in the presence of 0.8 mM Mg2+ Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.63 2-hydroxyphytanoyl-CoA Rattus norvegicus
-
 pristanal + formyl-CoA
-
 ?
4.1.2.63 2-hydroxyphytanoyl-CoA Homo sapiens
-
 pristanal + formyl-CoA
-
 ?
4.1.2.63 a 2-hydroxy-3-methyl-Cn-fatty-acyl-CoA Rattus norvegicus
-
 a 2-methyl-branched Cn-1-fatty aldehyde + formyl-CoA
-
 ?
4.1.2.63 a 2-hydroxy-3-methyl-Cn-fatty-acyl-CoA Homo sapiens
-
 a 2-methyl-branched Cn-1-fatty aldehyde + formyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.63 Homo sapiens Q9UJ83
-
-
4.1.2.63 Rattus norvegicus Q8CHM7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.63 DEAE-Sepharose column chromatography, phosphocellulose chromatography, and Ultrogel AcA-34 gel filtration Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.2.63 kidney highest expression in liver and kidney Homo sapiens
-
4.1.2.63 liver
-
 Rattus norvegicus
-
4.1.2.63 liver highest expression in liver and kidney Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.63 2-hydroxy-3-methylhexadecanoyl-CoA
-
 Rattus norvegicus 2-methylpentadecanal + formyl-CoA
-
 ?
4.1.2.63 2-hydroxy-3-methylhexadecanoyl-CoA
-
 Homo sapiens 2-methylpentadecanal + formyl-CoA
-
 ?
4.1.2.63 2-hydroxyphytanoyl-CoA
-
 Rattus norvegicus pristanal + formyl-CoA
-
 ?
4.1.2.63 2-hydroxyphytanoyl-CoA
-
 Homo sapiens pristanal + formyl-CoA
-
 ?
4.1.2.63 a 2-hydroxy-3-methyl-Cn-fatty-acyl-CoA
-
 Rattus norvegicus a 2-methyl-branched Cn-1-fatty aldehyde + formyl-CoA
-
 ?
4.1.2.63 a 2-hydroxy-3-methyl-Cn-fatty-acyl-CoA
-
 Homo sapiens a 2-methyl-branched Cn-1-fatty aldehyde + formyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.2.63 homotetramer 4 * 63000, SDS-PAGE Rattus norvegicus
4.1.2.63 homotetramer 4 * 63000, SDS-PAGE Homo sapiens
4.1.2.63 homotetramer 4 * 63732, calculated from amino acid sequence Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
4.1.2.63 2-HPCL
-
 Rattus norvegicus
4.1.2.63 2-HPCL
-
 Homo sapiens
4.1.2.63 2-hydroxyphytanoyl-CoA lyase
-
 Rattus norvegicus
4.1.2.63 2-hydroxyphytanoyl-CoA lyase
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.63 7.5 8 in Tris buffer Rattus norvegicus
4.1.2.63 7.5 8 in Tris buffer Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.2.63 thiamine diphosphate dependent on Rattus norvegicus
4.1.2.63 thiamine diphosphate dependent on Homo sapiens