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Literature summary extracted from

  • Donini, S.; Garavaglia, S.; Ferraris, D.M.; Miggiano, R.; Mori, S.; Shibayama, K.; Rizzi, M.
    Biochemical and structural investigations on phosphoribosylpyrophosphate synthetase from Mycobacterium smegmatis (2017), PLoS ONE, 12, e0175815 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.6.1 phosphate enzyme requires phosphate, optimal concentration ranges from 30 mM to 50 mM Mycolicibacterium smegmatis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.6.1 to 0.98 A resolution, structural comparison with human enzyme Mycolicibacterium smegmatis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.6.1 ADP
-
 Mycolicibacterium smegmatis
2.7.6.1 GDP 50% residual activity at 5 mM Mycolicibacterium smegmatis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.6.1 0.058
-
 ATP S0.5 value, Hill coefficient 1.7, pH 7.5, 25°C Mycolicibacterium smegmatis
2.7.6.1 0.059
-
 D-ribose 5-phosphate pH 7.5, 25°C Mycolicibacterium smegmatis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.6.1 Mg2+ enzyme is allosterically activated by Mg2+, S0.5 is 4mM, Hill coefficient is 2 Mycolicibacterium smegmatis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.6.1 221000
-
 gel filtration Mycolicibacterium smegmatis

Organism

EC Number Organism UniProt Comment Textmining
2.7.6.1 Mycolicibacterium smegmatis A0R3C8
-
-
2.7.6.1 Mycolicibacterium smegmatis ATCC 700084 A0R3C8
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.6.1 ATP + D-ribose 5-phosphate
-
 Mycolicibacterium smegmatis AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?
2.7.6.1 ATP + D-ribose 5-phosphate
-
 Mycolicibacterium smegmatis ATCC 700084 AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?
2.7.6.1 additional information diphosphate donors GTP, CTP and UTP are not processed Mycolicibacterium smegmatis ?
-
-
2.7.6.1 additional information diphosphate donors GTP, CTP and UTP are not processed Mycolicibacterium smegmatis ATCC 700084 ?
-
-

Subunits

EC Number Subunits Comment Organism
2.7.6.1 hexamer 6 * 35559, calculated from sequence Mycolicibacterium smegmatis

Synonyms

EC Number Synonyms Comment Organism
2.7.6.1 PrsA
-
 Mycolicibacterium smegmatis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.6.1 77.6
-
 D-ribose 5-phosphate pH 7.5, 25°C Mycolicibacterium smegmatis
2.7.6.1 83.5
-
 ATP pH 7.5, 25°C Mycolicibacterium smegmatis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.6.1 8
-
-
 Mycolicibacterium smegmatis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.6.1 9
-
 50% residual activity Mycolicibacterium smegmatis

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.6.1 0.174
-
 pH 7.5, 25°C Mycolicibacterium smegmatis ADP

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.6.1 1315
-
 D-ribose 5-phosphate pH 7.5, 25°C Mycolicibacterium smegmatis
2.7.6.1 1439
-
 ATP Hill coefficient 1.7, pH 7.5, 25°C Mycolicibacterium smegmatis