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Literature summary extracted from

  • Chen, F.; Zhang, Y.; Daugherty, A.B.; Yang, Z.; Shaw, R.; Dong, M.; Lutz, S.; Benner, S.A.
    Biological phosphorylation of an unnatural base pair (UBP) using a Drosophila melanogaster deoxynucleoside kinase (DmdNK) mutant (2017), PLoS ONE, 12, e0174163 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.145 expressed in Escherichia coli BL21(DE3) cells Drosophila melanogaster

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.145 Q81E the mutant enzyme shows higher phosphorylating activity with 6-amino-5-nitro-3-(1'-beta-D-2'-deoxyribofuranosyl)-2(1H)-pyridone as compared to the wild type enzyme Drosophila melanogaster

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.145 0.00068
-
 6-amino-5-nitro-3-(1'-beta-D-2'-deoxyribofuranosyl)-2(1H)-pyridone mutant enzyme Q81E, at pH 7.6 and 37°C Drosophila melanogaster
2.7.1.145 0.0087
-
 2'-deoxycytidine wild type enzyme, at pH 7.6 and 37°C Drosophila melanogaster
2.7.1.145 0.0113
-
 2'-deoxycytidine mutant enzyme Q81E, at pH 7.6 and 37°C Drosophila melanogaster
2.7.1.145 0.0217
-
 2-amino-8-(1'-beta-D-2'-deoxyribofuranosyl)-imidazo[1,2-a]-1,3,5-triazin-4(8H)-one mutant enzyme Q81E, at pH 7.6 and 37°C Drosophila melanogaster
2.7.1.145 0.09
-
 2-amino-8-(1'-beta-D-2'-deoxyribofuranosyl)-imidazo[1,2-a]-1,3,5-triazin-4(8H)-one wild type enzyme, at pH 7.6 and 37°C Drosophila melanogaster
2.7.1.145 0.1017
-
 6-amino-5-nitro-3-(1'-beta-D-2'-deoxyribofuranosyl)-2(1H)-pyridone wild type enzyme, at pH 7.6 and 37°C Drosophila melanogaster
2.7.1.145 0.4307
-
 2'-deoxyguanosine wild type enzyme, at pH 7.6 and 37°C Drosophila melanogaster
2.7.1.145 1.612
-
 2'-deoxyguanosine mutant enzyme Q81E, at pH 7.6 and 37°C Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.145 ATP + a 2'-deoxyribonucleoside Drosophila melanogaster
-
 ADP + a 2'-deoxyribonucleoside 5'-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.145 Drosophila melanogaster Q9XZT6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.145 chelating Sepharose column chromatography and Superdex S200 gel filtration Drosophila melanogaster

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.145 ATP + 2'-deoxycytidine
-
 Drosophila melanogaster ADP + 2'-deoxycytidine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxyguanosine
-
 Drosophila melanogaster ADP + 2'-deoxyguanosine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2-amino-8-(1'-beta-D-2'-deoxyribofuranosyl)-imidazo[1,2-a]-1,3,5-triazin-4(8H)-one
-
 Drosophila melanogaster ADP + 2-amino-8-(1'-beta-D-2'-deoxyribofuranosyl)-imidazo[1,2-a]-1,3,5-triazin-4(8H)-one 5'-phosphate
-
 ?
2.7.1.145 ATP + 6-amino-5-nitro-3-(1'-beta-D-2'-deoxyribofuranosyl)-2(1H)-pyridone
-
 Drosophila melanogaster ADP + 6-amino-5-nitro-3-(1'-beta-D-2'-deoxyribofuranosyl)-2(1H)-pyridone 5'-phosphate
-
 ?
2.7.1.145 ATP + a 2'-deoxyribonucleoside
-
 Drosophila melanogaster ADP + a 2'-deoxyribonucleoside 5'-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.145 dNK
-
 Drosophila melanogaster

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.145 0.00583
-
 6-amino-5-nitro-3-(1'-beta-D-2'-deoxyribofuranosyl)-2(1H)-pyridone wild type enzyme, at pH 7.6 and 37°C Drosophila melanogaster
2.7.1.145 0.067
-
 6-amino-5-nitro-3-(1'-beta-D-2'-deoxyribofuranosyl)-2(1H)-pyridone mutant enzyme Q81E, at pH 7.6 and 37°C Drosophila melanogaster
2.7.1.145 0.728
-
 2'-deoxyguanosine mutant enzyme Q81E, at pH 7.6 and 37°C Drosophila melanogaster
2.7.1.145 0.817
-
 2-amino-8-(1'-beta-D-2'-deoxyribofuranosyl)-imidazo[1,2-a]-1,3,5-triazin-4(8H)-one wild type enzyme, at pH 7.6 and 37°C Drosophila melanogaster
2.7.1.145 1.283
-
 2'-deoxyguanosine wild type enzyme, at pH 7.6 and 37°C Drosophila melanogaster
2.7.1.145 1.398
-
 2-amino-8-(1'-beta-D-2'-deoxyribofuranosyl)-imidazo[1,2-a]-1,3,5-triazin-4(8H)-one mutant enzyme Q81E, at pH 7.6 and 37°C Drosophila melanogaster
2.7.1.145 1.433
-
 2'-deoxycytidine wild type enzyme, at pH 7.6 and 37°C Drosophila melanogaster
2.7.1.145 2.1
-
 2'-deoxycytidine mutant enzyme Q81E, at pH 7.6 and 37°C Drosophila melanogaster

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.145 0.0583
-
 6-amino-5-nitro-3-(1'-beta-D-2'-deoxyribofuranosyl)-2(1H)-pyridone wild type enzyme, at pH 7.6 and 37°C Drosophila melanogaster
2.7.1.145 0.45
-
 2'-deoxyguanosine mutant enzyme Q81E, at pH 7.6 and 37°C Drosophila melanogaster
2.7.1.145 3
-
 2'-deoxyguanosine wild type enzyme, at pH 7.6 and 37°C Drosophila melanogaster
2.7.1.145 9
-
 2-amino-8-(1'-beta-D-2'-deoxyribofuranosyl)-imidazo[1,2-a]-1,3,5-triazin-4(8H)-one wild type enzyme, at pH 7.6 and 37°C Drosophila melanogaster
2.7.1.145 65
-
 2-amino-8-(1'-beta-D-2'-deoxyribofuranosyl)-imidazo[1,2-a]-1,3,5-triazin-4(8H)-one mutant enzyme Q81E, at pH 7.6 and 37°C Drosophila melanogaster
2.7.1.145 98.3
-
 6-amino-5-nitro-3-(1'-beta-D-2'-deoxyribofuranosyl)-2(1H)-pyridone mutant enzyme Q81E, at pH 7.6 and 37°C Drosophila melanogaster
2.7.1.145 165
-
 2'-deoxycytidine wild type enzyme, at pH 7.6 and 37°C Drosophila melanogaster
2.7.1.145 183.3
-
 2'-deoxycytidine mutant enzyme Q81E, at pH 7.6 and 37°C Drosophila melanogaster