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Literature summary extracted from

  • Liu, W.; Wang, L.; Fabian, P.; Hayashi, Y.; McGinley, C.; van der Donk, W.; Kulmacz, R.
    Arabidopsisxa0thaliana fatty acid alpha-dioxygenase-1 evaluation ofxa0substrates, inhibitors andxa0amino-terminal function (2006), Plant Physiol. Biochem., 44, 284-293 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.92 expression in Escherichia coli Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.92 3,4-dehydromyristate inactivates the oxygenase without appreciable oxygen consumption Arabidopsis thaliana
1.13.11.92 3-thiamyristate reaction leads to self-inactivation of enzyme Arabidopsis thaliana
1.13.11.92 catechol
-
 Arabidopsis thaliana
1.13.11.92 Co2+
-
 Arabidopsis thaliana
1.13.11.92 cyanide
-
 Arabidopsis thaliana
1.13.11.92 N,N,N',N'-tetramethyl-4-phenylenediamine
-
 Arabidopsis thaliana
1.13.11.92 Zn2+ presence of Zn2+ does not change the Km values for linoleate or oxygen, or the Ki value for cyanide Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.92 Arabidopsis thaliana Q9SGH6 isoform DOX1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.92 recombinant protein Arabidopsis thaliana

Storage Stability

EC Number Storage Stability Organism
1.13.11.92 4°C, 6 months, 90% residual activity Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.92 3-thiamyristate + O2
-
 Arabidopsis thaliana (2R)-2-hydroperoxy-3-thiatetradecanoic acid
-
 ?
1.13.11.92 gamma-linolenic acid + O2
-
 Arabidopsis thaliana ?
-
 ?
1.13.11.92 linoleic acid + O2
-
 Arabidopsis thaliana (2R)-2-hydroperoxy-(9Z,12Z)-octadeca-9,12-dienoic acid
-
 ?
1.13.11.92 linolenic acid + O2
-
 Arabidopsis thaliana (2R)-2-hydroperoxy-(9Z,12Z,15Z)-octadeca-9,12,15-trienoic acid
-
 ?
1.13.11.92 additional information no substrates: methyl esters of myristate, linoleate and linolenate are not oxygenated Arabidopsis thaliana ?
-
-
1.13.11.92 myristic acid + O2
-
 Arabidopsis thaliana (2R)-2-hydroperoxy-tetradecanoic acid
-
 ?
1.13.11.92 oleic acid + O2
-
 Arabidopsis thaliana (2R)-2-hydroperoxy-(9Z)-octadeca-9-enoic acid
-
 ?
1.13.11.92 palmitic acid + O2
-
 Arabidopsis thaliana (R,S)-2-hydroperoxypalmitic acid
-
 ?
1.13.11.92 palmitoleic acid + O2
-
 Arabidopsis thaliana (R,S)-2-hydroperoxypalmitic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.92 ? x * 98000, SDS-PAGE, recombinant GST-fusion protein Arabidopsis thaliana
1.13.11.92 More PADOX1 cleaved from GST-PADOX1 fusion by thrombin forms large multimeric complexes of 6-10 monomers. Full length PADOX1 expressed via a pCW vector in bacteria is a tetramer. Full length PADOX1 expressed in Sf9 cells chromatographed as a monomer Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.92 7.8
-
-
 Arabidopsis thaliana

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.13.11.92 0.005
-
 cyanide 30°C, pH 7.8 Arabidopsis thaliana
1.13.11.92 0.01
-
 Co2+ 30°C, pH 7.8 Arabidopsis thaliana
1.13.11.92 0.02
-
 N,N,N',N'-tetramethyl-4-phenylenediamine 30°C, pH 7.8 Arabidopsis thaliana
1.13.11.92 0.05
-
 Zn2+ 30°C, pH 7.8 Arabidopsis thaliana
1.13.11.92 0.09
-
 catechol 30°C, pH 7.8 Arabidopsis thaliana