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Literature summary extracted from

  • Takahashi, D.; Sakane, F.
    Expression and purification of human diacylglycerol kinase alpha from baculovirus-infected insect cells for structural studies (2018), PeerJ, 6, e5449 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.107 expressed in Escherichia coli strain Rosetta2 (DE3) and baculovirus-infected Sf9 insect cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.107 5-[(2E)-3-(2-furyl)prop-2-enylidene]-3-[(phenylsulfonyl)amino]2-thioxo-1,3-thiazolidin-4-one i.e. CU-3, ATP competitive inhibitor Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.107 0.24
-
 ATP isoform DGKalpha, at pH 7.4 and 30°C Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.107 Ca2+ 0.001 mM used in assay conditions. The enzyme is positively regulated by Ca2+ Homo sapiens
2.7.1.107 Mg2+ 10 mM used in assay conditions Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.107 ATP + 1,2-diacyl-sn-glycerol Homo sapiens
-
 ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.107 Homo sapiens P23743
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.107 Ni-NTA agarose column chromatography, Superdex 200 gel filtration or Superdex 75 gel filtration Homo sapiens

Storage Stability

EC Number Storage Stability Organism
2.7.1.107 4°C, purified enzyme in 20 mM Tris-HCl, pH 7.4 with 200 mM NaCl, 3 mM CaCl2, 3 mM MgCl2, 0.5 mM dithiothreitol, and 5% (v/v) glycerol, at least 3 months, no loss of activity Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.107 ATP + 1,2-diacyl-sn-glycerol
-
 Homo sapiens ADP + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?
2.7.1.107 ATP + 1,2-dioleolyl-sn-glycerol
-
 Homo sapiens ADP + 1,2-dioleolyl-sn-glycerol 3-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.107 ? x * 85000, His6-tagged enzyme, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.7.1.107 DGKalpha isoform Homo sapiens
2.7.1.107 diacylglycerol kinase alpha isoform Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.1.107 0.00034
-
 isoform DGKalpha, at pH 7.4 and 30°C Homo sapiens 5-[(2E)-3-(2-furyl)prop-2-enylidene]-3-[(phenylsulfonyl)amino]2-thioxo-1,3-thiazolidin-4-one