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Literature summary extracted from

  • Cheng, L.; Li, F.; Jiang, Y.; Yu, H.; Xie, C.; Shi, Y.; Gong, Q.
    Structural insights into a unique preference for 3 terminal guanine of mirtron in Drosophila TUTase Tailor (2019), Nucleic Acids Res., 47, 495-508 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.52 expressed in Escherichia coli Gold (DE3) cells Drosophila melanogaster

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.7.52 enzyme residues 202-560 and enzyme in complex with RNA stretches 5 -AGU-3 and 5 -AGUU-3 , sitting drop vapor diffusion method, using 0.1 M Tris-HCl, pH 8.4, 50 mM NaCl, 0.2 M lithium sulfate monohydrate and 15% (w/v) PEG3350 Drosophila melanogaster

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.52 H294A the enzymatic activity of the mutant is reduced by about 61% as compared to the wild type enzyme Drosophila melanogaster
2.7.7.52 N347A the uridylation efficiency of the mutant is reduced dramatically to about 7% when compared with that of wild type enzyme Drosophila melanogaster
2.7.7.52 R295A the enzymatic activity of the mutant is reduced by about 84% as compared to the wild type enzyme Drosophila melanogaster
2.7.7.52 R295K the enzymatic activity of the mutant is reduced by about 80% as compared to the wild type enzyme Drosophila melanogaster
2.7.7.52 R327A the uridylation efficiency of the mutant is reduced dramatically to about 4% when compared with that of wild type enzyme Drosophila melanogaster
2.7.7.52 R327K the uridylation efficiency of the mutant is reduced dramatically to about 4% when compared with that of wild type enzyme Drosophila melanogaster
2.7.7.52 V328I the mutation barely affects the enzyme's binding with RNA substrate and also exhibits a obvious reduction in uridylation efficiency as compared to the wild type enzyme Drosophila melanogaster
2.7.7.52 V328L the mutation entirely abolishes the enzyme's catalytic activity for truncated miR-1003 bearing 3'G. The mutation barely affects the enzyme's binding with RNA substrate Drosophila melanogaster
2.7.7.52 V328R the mutation entirely abolishes the enzyme's catalytic activity for truncated miR-1003 bearing 3'G. The mutation barely affects the enzyme's binding with RNA substrate Drosophila melanogaster

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.52 Mg2+ required Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.52 UTP + miR-1003 RNAn Drosophila melanogaster
-
 diphosphate + miR-1003 RNAn+1
-
 ?
2.7.7.52 UTP + RNAn Drosophila melanogaster the enzyme exhibits an intrinsic preference for RNA substrates ending in 3'G diphosphate + RNAn+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.52 Drosophila melanogaster Q9VI58
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.52 Ni-NTA column chromatography, HiTrap heparin column chromatography and Superdex 200 gel filtration Drosophila melanogaster

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.52 UTP + 5'-Cy5-GUGGGUAUCUGGGAGAUUACAUAUUCACAG-3'
-
 Drosophila melanogaster ?
-
 ?
2.7.7.52 UTP + 5-Cy5-CAUAUUCACAG-3'
-
 Drosophila melanogaster ?
-
 ?
2.7.7.52 UTP + miR-1003 RNAn
-
 Drosophila melanogaster diphosphate + miR-1003 RNAn+1
-
 ?
2.7.7.52 UTP + mirtronRNAn the enzyme preferentially catalyzes the uridylation of mirtronRNAs ending in 3'G and 3'U Drosophila melanogaster diphosphate + mirtronRNAn+1
-
 ?
2.7.7.52 UTP + RNAn the enzyme exhibits an intrinsic preference for RNA substrates ending in 3'G Drosophila melanogaster diphosphate + RNAn+1
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.52 Tailor
-
 Drosophila melanogaster
2.7.7.52 terminal uridylyl transferase
-
 Drosophila melanogaster
2.7.7.52 TUTase
-
 Drosophila melanogaster

General Information

EC Number General Information Comment Organism
2.7.7.52 metabolism the enzyme plays a crucial role as the repressor in the biogenesis pathway of splicing-derived mirtron pre-miRNAs Drosophila melanogaster