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Literature summary extracted from

  • Kroupova, A.; Ivascu, A.; Reimao-Pinto, M.M.; Ameres, S.L.; Jinek, M.
    Structural basis for acceptor RNA substrate selectivity of the 3 terminal uridylyl transferase Tailor (2019), Nucleic Acids Res., 47, 1030-1042 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.52 expressed in Sf9 insect cells Drosophila melanogaster

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.7.52 enzyme bound to uridine-5'-[(alpha,beta)-imido]triphosphate, hanging drop vapor diffusion method, using 20% (v/v) glycerol ethoxylate. Enzyme bound to GpU, sitting drop vapor diffusion method, using 30% (v/v) glycerol ethoxylate and 60 mM sodium malonate, pH 7.0. Enzyme bound to CACAGU, hanging drop vapor diffusion method, using 35% (v/v) glycerol ethoxylate and 75 mM sodium malonate, pH 7.0. Enzyme bound to U6 RNA, sitting drop vapor diffusion method, using 35% (v/v) glycerol ethoxylate and 90 mM sodium malonate, pH 7.0 Drosophila melanogaster

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.52 D280A the mutation abrogates enzyme activity Drosophila melanogaster
2.7.7.52 Q519A the activity of the mutant is reduced compared to the wild type enzyme. The mutant tails 3'-G, 3'-A and 3'-C substrates with comparable efficiencies Drosophila melanogaster
2.7.7.52 R327A the activity of the mutant is reduced compared to the wild type enzyme. The mutant shows clear abrogation of the 3'-G specificity, whereby the 3'-G substrate is uridylated less readily than the 3'-U, 3'-A and 3'-C substrates Drosophila melanogaster
2.7.7.52 R327K the activity of the mutant is reduced compared to the wild type enzyme but the mutant shows selectivity for 3'-G and 3'-U RNA substrates, similar to the wild type enzyme Drosophila melanogaster

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.52 Mg2+ 2 mM used in assay conditions Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.52 UTP + RNAn Drosophila melanogaster
-
 diphosphate + RNAn+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.52 Drosophila melanogaster Q9VI58
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.52 Ni-NTA resin column chromatography and Superdex 200 gel filtration Drosophila melanogaster

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.52 additional information the enzyme exhibits significantly higher tailing efficiency for substrates with a 3'-terminal G or U Drosophila melanogaster ?
-
-
2.7.7.52 UTP + miR-1003-3p-G 22-nt single stranded RNA oligonucleotide derived from the miR-1003 stem-loop Drosophila melanogaster ?
-
 ?
2.7.7.52 UTP + RNAn
-
 Drosophila melanogaster diphosphate + RNAn+1
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.52 3' terminal uridylyl transferase
-
 Drosophila melanogaster
2.7.7.52 Tailor
-
 Drosophila melanogaster
2.7.7.52 TUTase
-
 Drosophila melanogaster