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Literature summary extracted from

  • Li, D.; Tang, N.; Liu, M.; Shen, C.; Hu, Y.; Xia, Y.; Cao, M.
    Using hygromycin phosphotransferase and enhanced green fluorescent protein genes for tracking plastid transformation in rice (Oryza sativa L.) via gold particle bombardment (2016), Nanosci. Nanotechnol. Lett., 8, 409-417 .
No PubMed abstract available

Application

EC Number Application Comment Organism
2.7.1.119 biotechnology use the hygromycin phosphotransferase gene (hpt) as a selective marker gene for tracking plastid transformation in rice (Oryza sativa) Streptomyces sp.
2.7.1.119 molecular biology use the hygromycin phosphotransferase gene (hpt) as a selective marker gene for tracking plastid transformation in rice (Oryza sativa) Streptomyces sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.119 recombinant gene expression in transgenic Oryza sativa from site-specific transformation vector of rice plastid, pCTE04 Streptomyces sp.

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.119 additional information use the hygromycin phosphotransferase gene (hpt) as a selective marker gene for tracking plastid transformation in rice. A site-specific transformation vector of rice plastid, pCTE04, is constructed for dicistronic expression of the hygromycin phosphotransferase (hpt) and enhanced green fluorescent protein genes (EGFP). Transplastomic rice plants successfully grow up after a gradual selection of hygromycin resistance. Integration of the dicistronic expression cassette into the intergenic region trnI-trnA of plastid genome is verified by Southern blotting analysis. Transplastomic expression of the hpt and EGFP genes is identified by RT-PCR. The stable expression of dicistronic cassette in the rice is confirmed by confocal microscopy Streptomyces sp.

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.119 Streptomyces sp.
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Synonyms

EC Number Synonyms Comment Organism
2.7.1.119 hygromycin phosphotransferase
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 Streptomyces sp.