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Literature summary extracted from

  • Chen, K.; Guo, T.; Li, X.M.; Zhang, Y.M.; Yang, Y.B.; Ye, W.W.; Dong, N.Q.; Shi, C.L.; Kan, Y.; Xiang, Y.H.; Zhang, H.; Li, Y.C.; Gao, J.P.; Huang, X.; Zhao, Q.; Han, B.; Shan, J.X.; Lin, H.X.
    Translational regulation of plant response to high temperature by a dual-function tRNAHis guanylyltransferase in rice (2019), Mol. Plant, 12, 1123-1142 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.79 gene AET1, cloned from var. TQ, recombinant overexpression in Oryza sativa mediated by Agrobacterium tumefaciens strain EHA105 transformation, quantitative RT-PCR enzyme expression analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) pLysS, recombinant expression of MBP-tagged enzyme mutant P382S in Escherichia coli strain Rosetta-gami(DE3) Oryza sativa Japonica Group

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.79 additional information generation of an aet1 mutant, the aet1 mutant is crossed with a japonica variety, Jiahua-1, construction of CRISPR/Cas9 gene-editing constructs for AET1, RACK1A, eIF3h, and OsARF23. The aet1 mutant shows loss of Thg1 enzyme function, leading to reduced aminoacyl-tRNAHis levels and translational efficiency. The mutant plants display slight phenotype changes under normal conditions. In the aet1 mutant, the translation of OsARF19 and OsARF23 mRNAs is downregulated, which results in reduced levels of OsARFs and eventually causes growth arrest Oryza sativa Japonica Group

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.79 endoplasmic reticulum
-
 Oryza sativa Japonica Group 5783
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.79 Mg2+ required Oryza sativa Japonica Group

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.79 p-tRNAHis + ATP + GTP + H2O Oryza sativa Japonica Group overall reaction pGp-tRNAHis + AMP + 2 diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.79 Oryza sativa Japonica Group A0A0P0WQ58 cv. TeQing (TQ), cultivated in fields in Shanghai and Hainan
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.79 recombinnat His-tagged wild-type enzyme from Escherichia coli strain BL21(DE3) pLysS by nickel affinity chromatography, recombinant MBP-tagged enzyme mutant P382S from Escherichia coli strain Rosetta-gami(DE3) by amylose affinity chromatography Oryza sativa Japonica Group

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.79 p-tRNAHis + ATP + GTP + H2O overall reaction Oryza sativa Japonica Group pGp-tRNAHis + AMP + 2 diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.79 AET1
-
 Oryza sativa Japonica Group
2.7.7.79 THG1
-
 Oryza sativa Japonica Group

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.7.79 ATP
-
 Oryza sativa Japonica Group

General Information

EC Number General Information Comment Organism
2.7.7.79 malfunction an aet1 mutant shows loss of Thg1 enzyme function, leading to reduced aminoacyl-tRNAHis levels and translational efficiency. The mutant plants display slight phenotype changes under normal conditions. The translational status remains unaffected in the aet1 mutant, but the translational efficiency of OsARF19 and OsARF23 is reduced. Moreover, OsARF23 protein levels are obviously decreased in the aet1 mutant under high temperature, implying that AET1 regulates auxin signaling in response to high temperature Oryza sativa Japonica Group
2.7.7.79 additional information yeast two-hybrid assays with the AET1, RACK1A, and eIF3h proteins, and polysome profiling assays Oryza sativa Japonica Group
2.7.7.79 physiological function translational regulation of plant response to high temperature by a rice dual-function tRNAHis guanylyltransferase AET1, which contributes to the modification of pre-tRNAHis and is required for normal growth under high-temperature conditions in rice. AET1 possibly interacts with both RACK1A and eIF3h in the endoplasmic reticulum. AET1 can directly bind to OsARF mRNAs including the uORFs of OsARF19 and OsARF23, indicating that AET1 is associated with translation regulation. AET1 regulates auxin signaling in response to high temperature. AET1 regulates the environmental temperature response in rice by playing a dual role in tRNA modification and translational control. AET1 modulates auxin signaling through translation regulation associated with tRNA modification under high temperature, molecular mechanism, detailed overview. Under high temperature, AET1 can activate pre-tRNAHis and stabilize tRNA homeostasis. In particular, AET1 interacts with RACK1A and eIF3h and associates with the OsARF19 and OsARF23 mRNAs, which leads to increased translation of these mRNAs and contributes to plant development in response to high temperature Oryza sativa Japonica Group