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Literature summary extracted from

  • Rao, Z.; Kim, S.Y.; Li, X.; Kim, D.S.; Kim, Y.J.; Park, J.H.
    Insight into structural aspects of histidine 284 of Daphnia magna arginine kinase (2020), Mol. Cells, 43, 784-792 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.3.3
-
 Daphnia magna

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.3.3 hanging drop vapor diffusion method at 20°C. The crystal structure of H284A displays several structural changes, including the alteration of D324, a hydrogen-bonding network around H284, and the disruption of pi-stacking between the imidazole group of the H284 residue and the adenine ring of ATP Daphnia magna

Protein Variants

EC Number Protein Variants Comment Organism
2.7.3.3 H284A the catalytic activity is reduced significantly compared to that in wild type enzyme. The crystal structure of H284A displays several structural changes, including the alteration of D324, a hydrogen-bonding network around H284, and the disruption of pi-stacking between the imidazole group of the H284 residue and the adenine ring of ATP Daphnia magna

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.3.3 0.3
-
 ATP mutant enzyme H284A, pH and temperature not specified in the publication Daphnia magna
2.7.3.3 0.47
-
 ATP wild-type enzyme, pH and temperature not specified in the publication Daphnia magna
2.7.3.3 0.9
-
 L-arginine wild-type enzyme, pH and temperature not specified in the publication Daphnia magna
2.7.3.3 0.9
-
 L-arginine mutant enzyme H284A, pH and temperature not specified in the publication Daphnia magna

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.3.3 41000
-
 SDS-PAGE Daphnia magna

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.3.3 ATP + L-arginine Daphnia magna
-
 ADP + Nomega-phospho-L-arginine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.3.3 Daphnia magna A0A0A7CK57
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.3.3
-
 Daphnia magna

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.3.3 ATP + L-arginine
-
 Daphnia magna ADP + Nomega-phospho-L-arginine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.3.3 ? x * 41000, SDS-PAGE Daphnia magna

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.3.3 25
-
-
 Daphnia magna

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.3.3 0.19
-
 ATP mutant enzyme H284A, pH and temperature not specified in the publication Daphnia magna
2.7.3.3 0.19
-
 L-arginine mutant enzyme H284A, pH and temperature not specified in the publication Daphnia magna
2.7.3.3 18.6
-
 ATP wild-type enzyme, pH and temperature not specified in the publication Daphnia magna
2.7.3.3 18.6
-
 L-arginine wild-type enzyme, pH and temperature not specified in the publication Daphnia magna

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.3.3 8.5 9.5
-
 Daphnia magna

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.3.3 8 10 pH 8.0: about 60% of maximal activity, pH 10.0: about 70% of maximal activity Daphnia magna