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Literature summary extracted from

  • Aggarwal, N.; Ananthathamula, R.; Karanam, V.; Doble, M.; Chadha, A.
    Understanding substrate specificity and enantioselectivity of carbonyl reductase from Candida parapsilosis ATCC 7330 (CpCR) Experimental and modeling studies (2018), Mol. Catal., 460, 40-45 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.184
-
 Candida parapsilosis

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.184 Candida parapsilosis M4VRJ6
-
-
1.1.1.184 Candida parapsilosis ATCC 7330 M4VRJ6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.184 GST affinity column chromatography Candida parapsilosis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.184 0.01
-
 with acetophenone and NADH as substrates, at pH 7.0 and 30°C Candida parapsilosis
1.1.1.184 0.01
-
 with acetophenone and NADPH as substrates, at pH 7.0 and 30°C Candida parapsilosis
1.1.1.184 0.01
-
 with ethyl-4,4,4-trifloro-3-oxo-butanoate and NADPH as substrates, at pH 7.0 and 30°C Candida parapsilosis
1.1.1.184 0.02
-
 with 2-oxo-2-phenylacetaldehyde and NADH as substrates, at pH 7.0 and 30°C Candida parapsilosis
1.1.1.184 0.02
-
 with ethyl-4,4,4-trifloro-3-oxo-butanoate and NADH as substrates, at pH 7.0 and 30°C Candida parapsilosis
1.1.1.184 0.03
-
 with cinnamaldehyde and NADH as substrates, at pH 7.0 and 30°C Candida parapsilosis
1.1.1.184 0.45
-
 with 4-hydroxybenzaldehyde and NADPH as substrates, at pH 7.0 and 30°C Candida parapsilosis
1.1.1.184 0.47
-
 with 2-chlorobenzaldehyde and NADPH as substrates, at pH 7.0 and 30°C Candida parapsilosis
1.1.1.184 0.58
-
 with benzaldehyde and NADH as substrates, at pH 7.0 and 30°C Candida parapsilosis
1.1.1.184 0.62
-
 with 2-oxo-2-phenylacetic acid and NADPH as substrates, at pH 7.0 and 30°C Candida parapsilosis
1.1.1.184 0.64
-
 with ethyl-2-oxo-2-phenylacetate and NADH as substrates, at pH 7.0 and 30°C Candida parapsilosis
1.1.1.184 0.85
-
 with ethyl-2-oxo-2-phenylacetate and NADPH as substrates, at pH 7.0 and 30°C Candida parapsilosis
1.1.1.184 1.63
-
 with 2-oxo-2-phenylacetaldehyde and NADPH as substrates, at pH 7.0 and 30°C Candida parapsilosis
1.1.1.184 4.14
-
 with 3-hydroxybenzaldehyde and NADPH as substrates, at pH 7.0 and 30°C Candida parapsilosis
1.1.1.184 8.33
-
 with cinnamaldehyde and NADPH as substrates, at pH 7.0 and 30°C Candida parapsilosis
1.1.1.184 33.96
-
 with benzaldehyde and NADPH as substrates, at pH 7.0 and 30°C Candida parapsilosis
1.1.1.184 42.7
-
 with 1-butanal and NADH as substrates, at pH 7.0 and 30°C Candida parapsilosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.184 1-butanal + NADPH + H+
-
 Candida parapsilosis 1-butanol + NADP+
-
 ?
1.1.1.184 2-chlorobenzaldehyde + NADPH + H+
-
 Candida parapsilosis 2-chlorobenzyl alcohol + NADP+
-
 ?
1.1.1.184 2-chlorobenzaldehyde + NADPH + H+
-
 Candida parapsilosis ATCC 7330 2-chlorobenzyl alcohol + NADP+
-
 ?
1.1.1.184 2-oxo-2-phenylacetaldehyde + NADH + H+
-
 Candida parapsilosis ? + NAD+
-
 ?
1.1.1.184 2-oxo-2-phenylacetaldehyde + NADPH + H+ 11% conversion, more than 99% S-enantiomeric excess Candida parapsilosis (S)-1-phenylethane-1,2-diol + NADP+
-
 ?
1.1.1.184 2-oxo-2-phenylacetaldehyde + NADPH + H+
-
 Candida parapsilosis ? + NADP+
-
 ?
1.1.1.184 2-oxo-2-phenylacetic acid + NADPH + H+
-
 Candida parapsilosis ? + NADP+
-
 ?
1.1.1.184 3-hydroxybenzaldehyde + NADPH + H+
-
 Candida parapsilosis 3-hydroxybenzyl alcohol + NADP+
-
 ?
1.1.1.184 4-hydroxybenzaldehyde + NADPH + H+
-
 Candida parapsilosis 4-hydroxybenzyl alcohol + NADP+
-
 ?
1.1.1.184 4-hydroxybenzaldehyde + NADPH + H+
-
 Candida parapsilosis ATCC 7330 4-hydroxybenzyl alcohol + NADP+
-
 ?
1.1.1.184 acetophenone + NADH + H+
-
 Candida parapsilosis (R)-1-phenylethan-1-ol + NAD+
-
 ?
1.1.1.184 acetophenone + NADPH + H+ 88.5% conversion, 35% R-enantiomeric excess Candida parapsilosis (R)-1-phenylethan-1-ol + NADP+
-
 ?
1.1.1.184 benzaldehyde + NADH + H+
-
 Candida parapsilosis benzyl alcohol + NAD+
-
 ?
1.1.1.184 benzaldehyde + NADH + H+
-
 Candida parapsilosis ATCC 7330 benzyl alcohol + NAD+
-
 ?
1.1.1.184 benzaldehyde + NADPH + H+
-
 Candida parapsilosis benzyl alcohol + NADP+
-
 ?
1.1.1.184 benzaldehyde + NADPH + H+
-
 Candida parapsilosis ATCC 7330 benzyl alcohol + NADP+
-
 ?
1.1.1.184 cinnamaldehyde + NADH + H+
-
 Candida parapsilosis ? + NAD+
-
 ?
1.1.1.184 cinnamaldehyde + NADPH + H+
-
 Candida parapsilosis ? + NADP+
-
 ?
1.1.1.184 ethyl-2-oxo-2-phenylacetate + NADH + H+
-
 Candida parapsilosis ? + NAD+
-
 ?
1.1.1.184 ethyl-2-oxo-2-phenylacetate + NADPH + H+
-
 Candida parapsilosis ? + NADP+
-
 ?
1.1.1.184 ethyl-2-oxo-4-phenylbutanoate + NADPH + H+ 92% conversion, more than 99% R-enantiomeric excess Candida parapsilosis (R)-ethyl-2-hydroxy-4-phenylbutanoate + NADP+
-
 ?
1.1.1.184 ethyl-4,4,4-trifloro-3-oxo-butanoate + NADH + H+
-
 Candida parapsilosis ? + NAD+
-
 ?
1.1.1.184 ethyl-4,4,4-trifloro-3-oxo-butanoate + NADPH + H+ 15% conversion, 69% R-enantiomeric excess Candida parapsilosis (R)-ethyl-4,4,4-trifluoro-3-hydroxybutanoate + NADP+
-
 ?
1.1.1.184 ethyl-4,4,4-trifloro-3-oxo-butanoate + NADPH + H+
-
 Candida parapsilosis ? + NADP+
-
 ?
1.1.1.184 additional information the reduction of aldehydes is cofactor (NADPH) specific unlike the alpha-ketoesters which show dual specificity i.e. both NADH and NADPH Candida parapsilosis ?
-
-
1.1.1.184 additional information the reduction of aldehydes is cofactor (NADPH) specific unlike the alpha-ketoesters which show dual specificity i.e. both NADH and NADPH Candida parapsilosis ATCC 7330 ?
-
-

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.184 NADH
-
 Candida parapsilosis
1.1.1.184 NADPH
-
 Candida parapsilosis