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Literature summary extracted from

  • Fernández-Cabezón, L.; Galán, B.; García, J.
    Engineering Mycobacterium smegmatis for testosterone production (2017), Microb. Biotechnol., 10, 151-161 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.1.1.51 synthesis engineering Mycobacterium smegmatis for testosterone production. Mycobacterium smegmatis is an excellent chassis to develop biotechnological processes for the biotransformation of sterols and their derivatives into valuable pharmaceutical compounds. Overexpression of the gene encoding microbial 17beta-hydroxysteroid: NADP 17-oxidoreductase, from the bacterium Comamonas testosteroni. The host strains are Mycobacterium smegmatis wild type and a genetic engineered androst-4-ene-3,17-dione producing mutant. The recombinant strains are able to produce testosterone from androst-4-ene-3,17-dione and/or from sterols with high yields Comamonas testosteroni
1.1.1.51 synthesis engineering Mycobacterium smegmatis for testosterone production. Mycobacterium smegmatis is an excellent chassis to develop biotechnological processes for the biotransformation of sterols and their derivatives into valuable pharmaceutical compounds. Overexpression of the gene encoding microbial 17beta-hydroxysteroid: NADP 17-oxidoreductase, from the fungus Cochliobolus lunatus. The host strains are Mycobacterium smegmatis wild type and a genetic engineered androst-4-ene-3,17-dione producing mutant. The recombinant strains are able to produce testosterone from androst-4-ene-3,17-dione and/or from sterols with high yields Curvularia lunata

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.51
-
Curvularia lunata
1.1.1.51
-
Comamonas testosteroni

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.51 androst-4-ene-3,17-dione + NADPH + H+ Curvularia lunata
-
testosterone + NADP+
-
?
1.1.1.51 androst-4-ene-3,17-dione + NADPH + H+ Comamonas testosteroni
-
testosterone + NADP+
-
?
1.1.1.51 androst-4-ene-3,17-dione + NADPH + H+ Comamonas testosteroni ATCC 11996
-
testosterone + NADP+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.51 Comamonas testosteroni P19871
-
-
1.1.1.51 Comamonas testosteroni ATCC 11996 P19871
-
-
1.1.1.51 Curvularia lunata
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.51 androst-4-ene-3,17-dione + NADPH + H+
-
Curvularia lunata testosterone + NADP+
-
?
1.1.1.51 androst-4-ene-3,17-dione + NADPH + H+
-
Comamonas testosteroni testosterone + NADP+
-
?
1.1.1.51 androst-4-ene-3,17-dione + NADPH + H+
-
Comamonas testosteroni ATCC 11996 testosterone + NADP+
-
?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.51 17beta-HSD
-
Curvularia lunata
1.1.1.51 17beta-hydroxysteroid: NADP 17-oxidoreductase
-
Curvularia lunata
1.1.1.51 17beta-hydroxysteroid: NADP 17-oxidoreductase
-
Comamonas testosteroni
1.1.1.51 3beta/17beta-HSD
-
Comamonas testosteroni