EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.2.1.107 | cytoplasm | - |
Spinacia oleracea | 5737 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.2.1.107 | 150000 | - |
gel filtration | Spinacia oleracea |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.107 | D-glyceraldehyde 3-phosphate + arsenate + NAD+ | Spinacia oleracea | - |
1-arsono-3-phospho-D-glycerate + NADH + H+ | - |
ir |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.1.107 | Spinacia oleracea | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.1.107 | ammonium sulfate precipitation, acetone precipitation, NAD-Sepharose column chromatography, and Sephadex G-100 gel filtration, and DEAE-Sephadex gel filtration | Spinacia oleracea |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.2.1.107 | leaf | - |
Spinacia oleracea | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.2.1.107 | 80.4 | - |
purified enzyme, at pH 8.5 and 25°C | Spinacia oleracea |
EC Number | Storage Stability | Organism |
---|---|---|
1.2.1.107 | 20°C, purified enzyme, 6 months, about 70% loss of activity | Spinacia oleracea |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.107 | D-glyceraldehyde 3-phosphate + arsenate + NAD+ | - |
Spinacia oleracea | 1-arsono-3-phospho-D-glycerate + NADH + H+ | - |
ir |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.2.1.107 | homotetramer | 4 * 37000, SDS-PAGE | Spinacia oleracea |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.2.1.107 | 8.9 | 9.1 | - |
Spinacia oleracea |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.107 | NAD+ | - |
Spinacia oleracea |