Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yazawa, T.; Imamichi, Y.; Uwada, J.; Sekiguchi, T.; Mikami, D.; Kitano, T.; Ida, T.; Sato, T.; Nemoto, T.; Nagata, S.; Islam Khan, M.R.; Takahashi, S.; Ushikubi, F.; Suzuki, N.; Umezawa, A.; Taniguchi, T.
    Evaluation of 17beta-hydroxysteroid dehydrogenase activity using androgen receptor-mediated transactivation (2020), J. Steroid Biochem. Mol. Biol., 196, 105493 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.64 diagnostics evaluation of 17beta-HSD3 enzymatic activity using androgen receptor-mediated transactivation is important for understanding and diagnosing the 46,XY disorder of sexual development caused by mutations of HSD17B3 genes which results in low testosterone production. A method is adapted that easily evaluates enzymatic activity of 17beta-HSD3 by quantifying the conversion from androstenedione to trestosterone using androgen receptor-mediated transactivation Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.64 HEK-293 cells are transduced to express human HSD17B3. The system is also applicable to detect the conversion of 11-ketoandrostenedione to 11-ketotestosterone by HSD17B3 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.64 androstenedione + NADPH + H+ Homo sapiens
-
 testosterone + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.64 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.64 Leydig cell 17beta-HSD type 3 (HSD17B3) is expressed almost exclusively in testicular Leydig cells Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.64 androstenedione + NADPH + H+
-
 Homo sapiens testosterone + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.64 17beta-HSD type 3
-
 Homo sapiens
1.1.1.64 HSD17B3
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.64 NADPH
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
1.1.1.64 malfunction mutations of HSD17B3 genes cause a 46,XY disorder of sexual development (46,XY DSD) as a result of low testosterone production Homo sapiens
1.1.1.64 physiological function the enzyme (HSD17B3) contributes to development of male sexual characteristics by converting androstenedione (A4) to testosterone Homo sapiens