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Literature summary extracted from

  • Luzarowski, M.; Kosmacz, M.; Sokolowska, E.; Jasinska, W.; Willmitzer, L.; Veyel, D.; Skirycz, A.
    Affinity purification with metabolomic and proteomic analysis unravels diverse roles of nucleoside diphosphate kinases (2017), J. Exp. Bot., 68, 3487-3499 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.6 cloned into plant binary vector in which the constitutive 35S promoter is driving the expression of either C- or N-terminally tagged enzyme Arabidopsis thaliana
2.7.4.6 cloned into plant binary vector in which the constitutive 35S promoter is driving the expression of either C- or N-terminally tagged enzyme (NDPK) Arabidopsis thaliana

General Stability

EC Number General Stability Organism
2.7.4.6 ATP stabilizes the enzyme at a concentration of 0.01 mM Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.6 cAMP decreases the reaction speed of NDPK1 proportionally to the ligand concentration. Addition of 0.1 mM, 0.2 mM, and 0.5 mM cAMP reduces the specific activity of NDPK1 to 96%, 91%, and 84%, respectively, of the control Arabidopsis thaliana
2.7.4.6 cGMP decreases the reaction speed of NDPK1 proportionally to the ligand concentration. Addition of 0.1 mM, 0.2 mM, and 0.5 mM cGMP reduces the reaction speed to 84%, 69%, and 51% of the control Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.4.6 chloroplast NDPK2 is exclusively targeted to chloroplasts Arabidopsis thaliana 9507
-
2.7.4.6 chloroplast NDPK3 is localized to chloroplast Arabidopsis thaliana 9507
-
2.7.4.6 cytosol predominant localization of NDPK1 in cytosol Arabidopsis thaliana 5829
-

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.6 Arabidopsis thaliana O49203
-
-
2.7.4.6 Arabidopsis thaliana O64903
-
-
2.7.4.6 Arabidopsis thaliana P39207
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.4.6 glutathionylation NDPK1 undergoes glutathionylation at Cys43 Arabidopsis thaliana

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.6
-
Arabidopsis thaliana

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.6 12.1
-
pH and temperature not specified in the publication, NDPK1 Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.6 ATP + TDP
-
Arabidopsis thaliana ADP + TTP
-
?

Synonyms

EC Number Synonyms Comment Organism
2.7.4.6 At4g09320
-
Arabidopsis thaliana
2.7.4.6 At4g11010
-
Arabidopsis thaliana
2.7.4.6 At5g63310
-
Arabidopsis thaliana
2.7.4.6 NDPK1
-
Arabidopsis thaliana
2.7.4.6 NDPK2
-
Arabidopsis thaliana
2.7.4.6 NDPK3
-
Arabidopsis thaliana

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.4.6 additional information
-
3',5'-cAMP and 3',5'-cGMP at 1 mM stabilize NDPK1, shifting the Tm by 0.9-1.8°C Arabidopsis thaliana
2.7.4.6 60.3
-
Tm-value, nucleoside diphosphate kinase NDPK1, in water Arabidopsis thaliana
2.7.4.6 60.4
-
Tm-value, nucleoside diphosphate kinase NDPK1, 1 mM IMP, in water Arabidopsis thaliana
2.7.4.6 60.9
-
Tm-value, nucleoside diphosphate kinase NDPK1, 1 mM adenylosuccinate, in water Arabidopsis thaliana
2.7.4.6 62.6
-
Tm-value, nucleoside diphosphate kinase NDPK1, in 20 mM Tris-HCl (pH 8.0), 0.5 M NaCl, 20% glycerol Arabidopsis thaliana
2.7.4.6 62.7
-
Tm-value, nucleoside diphosphate kinase NDPK1, 0.01 mM ATP, in water Arabidopsis thaliana
2.7.4.6 62.7
-
Tm-value, nucleoside diphosphate kinase NDPK1, addition of 0.1 mM cAMP, in 20 mM Tris-HCl (pH 8.0), 0.5 M NaCl, 20% glycerol Arabidopsis thaliana
2.7.4.6 62.8
-
Tm-value, nucleoside diphosphate kinase NDPK1, addition of 0.1 mM cGMP, in 20 mM Tris-HCl (pH 8.0), 0.5 M NaCl, 20% glycerol Arabidopsis thaliana
2.7.4.6 63.5
-
Tm-value, nucleoside diphosphate kinase NDPK1, addition of 1 mM cAMP, in 20 mM Tris-HCl (pH 8.0), 0.5 M NaCl, 20% glycerol Arabidopsis thaliana
2.7.4.6 64.4
-
Tm-value, nucleoside diphosphate kinase NDPK1, addition of 1 mM cGMP, in 20 mM Tris-HCl (pH 8.0), 0.5 M NaCl, 20% glycerol Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
2.7.4.6 physiological function key enzyme regulating NDP/NTP homeostasis Arabidopsis thaliana