EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.4.6 | cloned into plant binary vector in which the constitutive 35S promoter is driving the expression of either C- or N-terminally tagged enzyme | Arabidopsis thaliana |
2.7.4.6 | cloned into plant binary vector in which the constitutive 35S promoter is driving the expression of either C- or N-terminally tagged enzyme (NDPK) | Arabidopsis thaliana |
EC Number | General Stability | Organism |
---|---|---|
2.7.4.6 | ATP stabilizes the enzyme at a concentration of 0.01 mM | Arabidopsis thaliana |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.4.6 | cAMP | decreases the reaction speed of NDPK1 proportionally to the ligand concentration. Addition of 0.1 mM, 0.2 mM, and 0.5 mM cAMP reduces the specific activity of NDPK1 to 96%, 91%, and 84%, respectively, of the control | Arabidopsis thaliana | |
2.7.4.6 | cGMP | decreases the reaction speed of NDPK1 proportionally to the ligand concentration. Addition of 0.1 mM, 0.2 mM, and 0.5 mM cGMP reduces the reaction speed to 84%, 69%, and 51% of the control | Arabidopsis thaliana |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.7.4.6 | chloroplast | NDPK2 is exclusively targeted to chloroplasts | Arabidopsis thaliana | 9507 | - |
2.7.4.6 | chloroplast | NDPK3 is localized to chloroplast | Arabidopsis thaliana | 9507 | - |
2.7.4.6 | cytosol | predominant localization of NDPK1 in cytosol | Arabidopsis thaliana | 5829 | - |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.4.6 | Arabidopsis thaliana | O49203 | - |
- |
2.7.4.6 | Arabidopsis thaliana | O64903 | - |
- |
2.7.4.6 | Arabidopsis thaliana | P39207 | - |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
2.7.4.6 | glutathionylation | NDPK1 undergoes glutathionylation at Cys43 | Arabidopsis thaliana |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.4.6 | - |
Arabidopsis thaliana |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.4.6 | 12.1 | - |
pH and temperature not specified in the publication, NDPK1 | Arabidopsis thaliana |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.4.6 | ATP + TDP | - |
Arabidopsis thaliana | ADP + TTP | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.4.6 | At4g09320 | - |
Arabidopsis thaliana |
2.7.4.6 | At4g11010 | - |
Arabidopsis thaliana |
2.7.4.6 | At5g63310 | - |
Arabidopsis thaliana |
2.7.4.6 | NDPK1 | - |
Arabidopsis thaliana |
2.7.4.6 | NDPK2 | - |
Arabidopsis thaliana |
2.7.4.6 | NDPK3 | - |
Arabidopsis thaliana |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.4.6 | additional information | - |
3',5'-cAMP and 3',5'-cGMP at 1 mM stabilize NDPK1, shifting the Tm by 0.9-1.8°C | Arabidopsis thaliana |
2.7.4.6 | 60.3 | - |
Tm-value, nucleoside diphosphate kinase NDPK1, in water | Arabidopsis thaliana |
2.7.4.6 | 60.4 | - |
Tm-value, nucleoside diphosphate kinase NDPK1, 1 mM IMP, in water | Arabidopsis thaliana |
2.7.4.6 | 60.9 | - |
Tm-value, nucleoside diphosphate kinase NDPK1, 1 mM adenylosuccinate, in water | Arabidopsis thaliana |
2.7.4.6 | 62.6 | - |
Tm-value, nucleoside diphosphate kinase NDPK1, in 20 mM Tris-HCl (pH 8.0), 0.5 M NaCl, 20% glycerol | Arabidopsis thaliana |
2.7.4.6 | 62.7 | - |
Tm-value, nucleoside diphosphate kinase NDPK1, 0.01 mM ATP, in water | Arabidopsis thaliana |
2.7.4.6 | 62.7 | - |
Tm-value, nucleoside diphosphate kinase NDPK1, addition of 0.1 mM cAMP, in 20 mM Tris-HCl (pH 8.0), 0.5 M NaCl, 20% glycerol | Arabidopsis thaliana |
2.7.4.6 | 62.8 | - |
Tm-value, nucleoside diphosphate kinase NDPK1, addition of 0.1 mM cGMP, in 20 mM Tris-HCl (pH 8.0), 0.5 M NaCl, 20% glycerol | Arabidopsis thaliana |
2.7.4.6 | 63.5 | - |
Tm-value, nucleoside diphosphate kinase NDPK1, addition of 1 mM cAMP, in 20 mM Tris-HCl (pH 8.0), 0.5 M NaCl, 20% glycerol | Arabidopsis thaliana |
2.7.4.6 | 64.4 | - |
Tm-value, nucleoside diphosphate kinase NDPK1, addition of 1 mM cGMP, in 20 mM Tris-HCl (pH 8.0), 0.5 M NaCl, 20% glycerol | Arabidopsis thaliana |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.7.4.6 | physiological function | key enzyme regulating NDP/NTP homeostasis | Arabidopsis thaliana |