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Literature summary extracted from

  • Cho, Y.; Yu, C.Y.; Nakamura, Y.; Kanehara, K.
    Arabidopsis dolichol kinase AtDOK1 is involved in flowering time control (2017), J. Exp. Bot., 68, 3243-3252 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.108 gene SEC59, Agrobacterium tumefaciens strain GV3101 is used to transform dok1-1 heterozygous plants with the Venus-tagged wild-type and mutant enzymes (DOK1-Venus fusion proteins), functional complementation of the Saccharomyces cerevisiae sec59 mutant, showing the temperature-sensitive growth phenotype, by expression of the His6-tagged Arabidopsis thaliana enzyme, the sec59 mutant expressing AtDOK1 (G741D) mutant fails to do so, quantitative RT-PCR expression analysis Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.108 G471D site-directed mutagenesis,inactive mutant Arabidopsis thaliana
2.7.1.108 additional information creation of leaky knockdown mutants of gene DOK1 by microRNA-mediated gene suppression technique. Knockout of gene DOK1 causes lethality, while the DOK1 knockdown mutants show an early flowering phenotype without any remarkable growth defect in vegetative tissues Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.108 endoplasmic reticulum membrane topology of AtDOK1 in the endoplasmic reticulum membrane, overview. AtDOK1 contains 13 transmembrane (TM) segments (TM1-TM13) with an N-terminus facing the endoplasmic reticulum lumen and a C-terminus facing the cytoplasm. The topological arrangement of AtDOK1 is similar to that of human enzyme Arabidopsis thaliana 5789
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2.7.1.108 additional information subcellular localization study Arabidopsis thaliana
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Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.108 CTP + dolichol Arabidopsis thaliana
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 CDP + dolichyl phosphate
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 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.108 Arabidopsis thaliana F4J4C8
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-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.108 flower floral meristem Arabidopsis thaliana
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2.7.1.108 inflorescence
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 Arabidopsis thaliana
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2.7.1.108 additional information the expression of AtDOK1 is limited to highly pluripotent cells although protein glycosylation is thought to be required ubiquitously in the entire plant body. AtDOK1 is highly expressed in emerging shoot apical meristems as well as inflorescence and floral meristems Arabidopsis thaliana
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2.7.1.108 seedling
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 Arabidopsis thaliana
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2.7.1.108 shoot apical meristem Arabidopsis thaliana
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.108 CTP + dolichol
-
 Arabidopsis thaliana CDP + dolichyl phosphate
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 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.108 At3g45040
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 Arabidopsis thaliana
2.7.1.108 AtDOK1
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 Arabidopsis thaliana
2.7.1.108 dolichol kinase 1
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 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.108 CTP
-
 Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
2.7.1.108 additional information AtDOK1 contains a DxxAxxxGxxxGx8KKTxEG motif that is conserved among enzymes utilizing CTP as a substrate, including hDOLK and yeast Sec59p. The motif contains the CTP binding site Arabidopsis thaliana
2.7.1.108 physiological function Arabidopsis thaliana dolichol kinase 1, AtDOK1, is functionally involved in plant reproductive processes. Dolichols are a class of isoprenoids that consist of highly polymerized and unsaturated long-chain isoprenes. They play crucial roles in protein glycosylation including N-glycosylation, because the oligosaccharide is assembled on a lipid carrier, dolichyl diphosphate. Dolichol phosphate mannose (Dol-P-Man) serves as a lipid carrier for the initial steps of N-glycosylation, O-mannosylation, and glycosylphosphatidylinositol (GPI) anchoring. The endoplasmic reticulum-localized catalytically active DOK1 is highly expressed in the meristems and is involved in the control of flowering time, possibly by post-transcriptional regulation including protein glycosylation. DOK1 may post-transcriptionally affect factors that regulate flowering time control because DOK is involved in protein glycosylation, including N-glycosylation, O-mannosylation, and GPI anchoring Arabidopsis thaliana