EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.36 | expressed in Escherichia coli BL21(DE3) cells | Candidatus Accumulibacter phosphatis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.36 | 0.0077 | - |
NADH | recombinant enzyme, at pH 7.5 and 25°C | Candidatus Accumulibacter phosphatis | |
1.1.1.36 | 0.0443 | - |
NADPH | recombinant enzyme, at pH 7.5 and 25°C | Candidatus Accumulibacter phosphatis | |
1.1.1.36 | 0.0567 | - |
acetoacetyl-CoA | recombinant enzyme, with NADH as cosubstrate, at pH 7.5 and 25°C | Candidatus Accumulibacter phosphatis | |
1.1.1.36 | 0.318 | - |
acetoacetyl-CoA | recombinant enzyme, with NADPH as cosubstrate, at pH 7.5 and 25°C | Candidatus Accumulibacter phosphatis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.36 | (R)-3-hydroxybutyryl-CoA + NAD+ | Candidatus Accumulibacter phosphatis | - |
acetoacetyl-CoA + NADH + H+ | - |
r | |
1.1.1.36 | (R)-3-hydroxybutyryl-CoA + NAD+ | Candidatus Accumulibacter phosphatis UW-1 | - |
acetoacetyl-CoA + NADH + H+ | - |
r | |
1.1.1.36 | acetoacetyl-CoA + NADH + H+ | Candidatus Accumulibacter phosphatis | - |
(R)-3-hydroxybutyryl-CoA + NAD+ | - |
r | |
1.1.1.36 | acetoacetyl-CoA + NADH + H+ | Candidatus Accumulibacter phosphatis UW-1 | - |
(R)-3-hydroxybutyryl-CoA + NAD+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.36 | Candidatus Accumulibacter phosphatis | C7RM91 | - |
- |
1.1.1.36 | Candidatus Accumulibacter phosphatis UW-1 | C7RM91 | - |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.36 | 0.07 | - |
native enzyme, forward reaction, using NADPH as cosubstrate, at pH 7.5 and 25°C | Candidatus Accumulibacter phosphatis |
1.1.1.36 | 0.25 | - |
recombinant enzyme, forward reaction, using NADPH as cosubstrate, at pH 7.5 and 25°C | Candidatus Accumulibacter phosphatis |
1.1.1.36 | 0.61 | - |
native enzyme, backward reaction, using NADH as cosubstrate, at pH 7.5 and 25°C | Candidatus Accumulibacter phosphatis |
1.1.1.36 | 1.96 | - |
recombinant enzyme, forward reaction, using NADH as cosubstrate, at pH 7.5 and 25°C | Candidatus Accumulibacter phosphatis |
1.1.1.36 | 14 | - |
native enzyme, forward reaction, using NADH as cosubstrate, at pH 7.5 and 25°C | Candidatus Accumulibacter phosphatis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.36 | (R)-3-hydroxybutyryl-CoA + NAD+ | - |
Candidatus Accumulibacter phosphatis | acetoacetyl-CoA + NADH + H+ | - |
r | |
1.1.1.36 | (R)-3-hydroxybutyryl-CoA + NAD+ | - |
Candidatus Accumulibacter phosphatis UW-1 | acetoacetyl-CoA + NADH + H+ | - |
r | |
1.1.1.36 | acetoacetyl-CoA + NADH + H+ | - |
Candidatus Accumulibacter phosphatis | (R)-3-hydroxybutyryl-CoA + NAD+ | - |
r | |
1.1.1.36 | acetoacetyl-CoA + NADH + H+ | - |
Candidatus Accumulibacter phosphatis UW-1 | (R)-3-hydroxybutyryl-CoA + NAD+ | - |
r | |
1.1.1.36 | acetoacetyl-CoA + NADPH + H+ | - |
Candidatus Accumulibacter phosphatis | (R)-3-hydroxybutyryl-CoA + NADP+ | - |
? | |
1.1.1.36 | acetoacetyl-CoA + NADPH + H+ | - |
Candidatus Accumulibacter phosphatis UW-1 | (R)-3-hydroxybutyryl-CoA + NADP+ | - |
? | |
1.1.1.36 | additional information | no oxidation of (R/S)-3-hydroxybutyryl-CoA is detected when using NADP+ as cofactor | Candidatus Accumulibacter phosphatis | ? | - |
- |
|
1.1.1.36 | additional information | no oxidation of (R/S)-3-hydroxybutyryl-CoA is detected when using NADP+ as cofactor | Candidatus Accumulibacter phosphatis UW-1 | ? | - |
- |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.36 | AAR | - |
Candidatus Accumulibacter phosphatis |
1.1.1.36 | NADH-preferring acetoacetyl-CoA reductase | - |
Candidatus Accumulibacter phosphatis |
1.1.1.36 | PhaB | - |
Candidatus Accumulibacter phosphatis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.36 | 0.095 | - |
NADPH | recombinant enzyme, at pH 7.5 and 25°C | Candidatus Accumulibacter phosphatis | |
1.1.1.36 | 0.131 | - |
acetoacetyl-CoA | recombinant enzyme, with NADPH as cosubstrate, at pH 7.5 and 25°C | Candidatus Accumulibacter phosphatis | |
1.1.1.36 | 8.92 | - |
NADH | recombinant enzyme, at pH 7.5 and 25°C | Candidatus Accumulibacter phosphatis | |
1.1.1.36 | 11.6 | - |
acetoacetyl-CoA | recombinant enzyme, with NADH as cosubstrate, at pH 7.5 and 25°C | Candidatus Accumulibacter phosphatis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.36 | NADH | the enzyme clearly prefers NADH over NADPH | Candidatus Accumulibacter phosphatis |