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Literature summary extracted from

  • Wahl, C.; Spiertz, M.; Elling, L.
    Characterization of a new UDP-sugar pyrophosphorylase from Hordeum vulgare (barley) (2017), J. Biotechnol., 258, 51-55 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.64 gene hvusp, identified due to a BLAST search using the gene psusp from Pisum sativum as template, recombinant expression of C-terminally His-tagged enzyme best in Escherichia coli strain T7 Express, several Escherichia coli strains are tested Hordeum vulgare subsp. vulgare

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.64 additional information UDP-sugar pyrophosphorylase from Hordeum vulgare, with favorable biochemical properties like broad pH and temperature tolerances as well as a broad substrate spectrum and high synthesis stability, is used for efficient synthesis of nucleotide sugars under conditions optimized by high-through-put multiplexed capillary electrophoresis analysis in combination with a galactokinase UDP-alpha-D-galactose (UDP-Gal). Assay optimization, overview Hordeum vulgare subsp. vulgare

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.64 ADP at higher concentrations above 6 mM Hordeum vulgare subsp. vulgare
2.7.7.64 ATP
-
 Hordeum vulgare subsp. vulgare
2.7.7.64 diphosphate
-
 Hordeum vulgare subsp. vulgare
2.7.7.64 EDTA complete inhibition Hordeum vulgare subsp. vulgare

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.64 additional information
-
 additional information Michaelis-Menten kinetics Hordeum vulgare subsp. vulgare
2.7.7.64 0.3
-
 alpha-D-galactose 1-phosphate pH 7.5, 37°C, recombinant enzyme Hordeum vulgare subsp. vulgare

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.64 Co2+ activates Hordeum vulgare subsp. vulgare
2.7.7.64 Mg2+ required, activates, preferred cation Hordeum vulgare subsp. vulgare
2.7.7.64 Mn2+ activates Hordeum vulgare subsp. vulgare
2.7.7.64 additional information the enzyme requires bivalent cations for activity. HvUSP accepts other bivalent cation such as Co2+, Ni2+, Mn2+ and Zn2+, but prefers Mg2+ Hordeum vulgare subsp. vulgare
2.7.7.64 Ni2+ activates Hordeum vulgare subsp. vulgare
2.7.7.64 Zn2+ activates Hordeum vulgare subsp. vulgare

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.64 UTP + alpha-D-galactose 1-phosphate Hordeum vulgare subsp. vulgare
-
 diphosphate + UDP-alpha-D-galactose
-
 r
2.7.7.64 UTP + alpha-D-glucose 1-phosphate Hordeum vulgare subsp. vulgare
-
 diphosphate + UDP-alpha-D-glucose
-
 r
2.7.7.64 UTP + alpha-D-glucuronate 1-phosphate Hordeum vulgare subsp. vulgare
-
 diphosphate + UDP-alpha-D-glucuronate
-
 r
2.7.7.64 UTP + D-xylose 1-phosphate Hordeum vulgare subsp. vulgare
-
 diphosphate + UDP-D-xylose
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.64 Hordeum vulgare subsp. vulgare F2DQG9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.64 recombinant His-tagged enzyme from Escherichia coli strain T7 Express by nickel affinity chromatography and ultrafiltration Hordeum vulgare subsp. vulgare

Storage Stability

EC Number Storage Stability Organism
2.7.7.64 4°C, purified recombinant His-taged enzyme, HvUSP shows a high storage stability over 31 days Hordeum vulgare subsp. vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.64 additional information enzyme HvUSP has a promiscuous substrate spectrum. It is not active with ATP, CTP, GTP or dTTP, and in combination with UTP HvUSP shows conversion in decreasing order of Gal-1-P, Glc-1-P, GlcA-1-P and Xyl-1-P. No activity with mannose-1phosphate, N-acetyl-glucosamine-1-phosphate, glucosamine-1-phosphate, or fucose-1-phosphate Hordeum vulgare subsp. vulgare ?
-
-
2.7.7.64 UTP + alpha-D-galactose 1-phosphate
-
 Hordeum vulgare subsp. vulgare diphosphate + UDP-alpha-D-galactose
-
 r
2.7.7.64 UTP + alpha-D-galactose 1-phosphate preferred substrates Hordeum vulgare subsp. vulgare diphosphate + UDP-alpha-D-galactose
-
 r
2.7.7.64 UTP + alpha-D-glucose 1-phosphate
-
 Hordeum vulgare subsp. vulgare diphosphate + UDP-alpha-D-glucose
-
 r
2.7.7.64 UTP + alpha-D-glucuronate 1-phosphate
-
 Hordeum vulgare subsp. vulgare diphosphate + UDP-alpha-D-glucuronate
-
 r
2.7.7.64 UTP + D-xylose 1-phosphate
-
 Hordeum vulgare subsp. vulgare diphosphate + UDP-D-xylose
-
 ir

Subunits

EC Number Subunits Comment Organism
2.7.7.64 ? x * 68700, about, sequence calculation Hordeum vulgare subsp. vulgare

Synonyms

EC Number Synonyms Comment Organism
2.7.7.64 HvUSP
-
 Hordeum vulgare subsp. vulgare
2.7.7.64 UDP-sugar pyrophosphorylase
-
 Hordeum vulgare subsp. vulgare

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.64 45
-
-
 Hordeum vulgare subsp. vulgare

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.7.64 35 60 50% of maximal activity within this range Hordeum vulgare subsp. vulgare

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.64 37
-
 purified recombinant His-taged enzyme, residual activity of HvUSP after 88 h is 46% Hordeum vulgare subsp. vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.64 7.5
-
-
 Hordeum vulgare subsp. vulgare

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.64 7 9.5 50% of maximal activity within this range Hordeum vulgare subsp. vulgare

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.7.64 Hordeum vulgare subsp. vulgare sequence calculation
-
 6.44

General Information

EC Number General Information Comment Organism
2.7.7.64 metabolism UDP-sugar pyrophosphorylases are involved in the plant salvage pathways Hordeum vulgare subsp. vulgare