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Literature summary extracted from

  • Han, X.; Hu, X.; Zhou, C.; Wang, H.; Li, Q.; Ouyang, Y.; Kuang, X.; Xiao, D.; Xiang, Q.; Yu, X.; Li, X.; Gu, Y.; Zhao, K.; Chen, Q.; Ma, M.
    Cloning and functional characterization of xylitol dehydrogenase genes from Issatchenkia orientalis and Torulaspora delbrueckii (2020), J. Biosci. Bioeng., 130, 29-35 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.9 additional information glycerol has not a strong effect on the enzyme activity at 1-10 mM Scheffersomyces stipitis
1.1.1.9 additional information glycerol has not a strong effect on the enzyme activity at 1-10 mM Pichia kudriavzevii
1.1.1.9 additional information glycerol has not a strong effect on the enzyme activity at 1-10 mM Torulaspora delbrueckii
1.1.1.9 NaCl gradually activates the enzyme with increasing concentrations of 0.1-1.0 M Pichia kudriavzevii

Application

EC Number Application Comment Organism
1.1.1.9 synthesis enzyme IoXyl2p from Issatchenkia orientalis is considered to be an attractive candidate for the construction of genetically engineered Saccharomyces cerevisiae for efficient fermentation of carbohydrate in lignocellulosic hydrolysate Pichia kudriavzevii
1.1.1.9 synthesis enzyme TdXyl2p from Torulaspora delbrueckii is considered to be an attractive candidate for the construction of genetically engineered Saccharomyces cerevisiae for efficient fermentation of carbohydrate in lignocellulosic hydrolysate Torulaspora delbrueckii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.9 gene Xyl2, DNA and amino acid sequence determination and analysis, sequence comparisons Pichia kudriavzevii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.9 2-mercaptoethanol slight inhibition Scheffersomyces stipitis
1.1.1.9 2-mercaptoethanol slight inhibition Torulaspora delbrueckii
1.1.1.9 EDTA strong inhibition at 1-10 mM Pichia kudriavzevii
1.1.1.9 EDTA strong inhibition at 1 mM, very strong inhibition at 5-10 mM Scheffersomyces stipitis
1.1.1.9 EDTA strong inhibition at 1-10 mM Torulaspora delbrueckii
1.1.1.9 KCl gradually inhibits the enzyme with increasing concentrations of 0.1-1.0 M Scheffersomyces stipitis
1.1.1.9 KCl gradually inhibits the enzyme with increasing concentrations of 0.1-1.0 M Torulaspora delbrueckii
1.1.1.9 Mg2+ slight inhibition Scheffersomyces stipitis
1.1.1.9 additional information glycerol has not a strong effect on the enzyme activity at 1-10 mM Pichia kudriavzevii
1.1.1.9 additional information glycerol has not a strong effect on the enzyme activity at 1-10 mM Scheffersomyces stipitis
1.1.1.9 additional information glycerol has not a strong effect on the enzyme activity at 1-10 mM Torulaspora delbrueckii
1.1.1.9 NaCl gradually inhibits the enzyme with increasing concentrations of 0.1-1.0 M Scheffersomyces stipitis
1.1.1.9 NaCl gradually inhibits the enzyme with increasing concentrations of 0.1-1.0 M Torulaspora delbrueckii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.9 10.38
-
 xylitol pH 7.0, 30°C, recombinant enzyme Pichia kudriavzevii
1.1.1.9 17.64
-
 xylitol pH 7.0, 30°C, recombinant enzyme Scheffersomyces stipitis
1.1.1.9 20.96
-
 xylitol pH 7.0, 30°C, recombinant enzyme Torulaspora delbrueckii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.9 cytoplasm
-
 Scheffersomyces stipitis 5737
-
1.1.1.9 cytoplasm
-
 Pichia kudriavzevii 5737
-
1.1.1.9 cytoplasm
-
 Torulaspora delbrueckii 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.9 Mg2+ activates Pichia kudriavzevii
1.1.1.9 Mg2+ activates Torulaspora delbrueckii
1.1.1.9 additional information KCl has not a strong effect on the enzyme activity at 0.1-1.0 Pichia kudriavzevii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.9 xylitol + NAD+ Scheffersomyces stipitis
-
 D-xylulose + NADH + H+
-
 r
1.1.1.9 xylitol + NAD+ Pichia kudriavzevii
-
 D-xylulose + NADH + H+
-
 r
1.1.1.9 xylitol + NAD+ Torulaspora delbrueckii
-
 D-xylulose + NADH + H+
-
 r
1.1.1.9 xylitol + NAD+ Pichia kudriavzevii QLB_09
-
 D-xylulose + NADH + H+
-
 r
1.1.1.9 xylitol + NAD+ Scheffersomyces stipitis NRRL Y-11545
-
 D-xylulose + NADH + H+
-
 r
1.1.1.9 xylitol + NAD+ Scheffersomyces stipitis NBRC 10063
-
 D-xylulose + NADH + H+
-
 r
1.1.1.9 xylitol + NAD+ Scheffersomyces stipitis ATCC 58785
-
 D-xylulose + NADH + H+
-
 r
1.1.1.9 xylitol + NAD+ Torulaspora delbrueckii BLQ_03
-
 D-xylulose + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.9 Pichia kudriavzevii A0A3S7PMB5 Issatchenkia orientalis, a multistress-tolerant yeast
-
1.1.1.9 Pichia kudriavzevii QLB_09 A0A3S7PMB5 Issatchenkia orientalis, a multistress-tolerant yeast
-
1.1.1.9 Scheffersomyces stipitis P22144 Pichia stipitis
-
1.1.1.9 Scheffersomyces stipitis ATCC 58785 P22144 Pichia stipitis
-
1.1.1.9 Scheffersomyces stipitis NBRC 10063 P22144 Pichia stipitis
-
1.1.1.9 Scheffersomyces stipitis NRRL Y-11545 P22144 Pichia stipitis
-
1.1.1.9 Torulaspora delbrueckii A0A3S7PMC4 Candida colliculosa, a multistress-tolerant yeast
-
1.1.1.9 Torulaspora delbrueckii BLQ_03 A0A3S7PMC4 Candida colliculosa, a multistress-tolerant yeast
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.9 10.71
-
 pH 7.0, 30°C, recombinant enzyme with xylitol and NAD+ as substrates Torulaspora delbrueckii
1.1.1.9 13.29
-
 pH 7.0, 30°C, recombinant enzyme with xylitol and NAD+ as substrates Scheffersomyces stipitis
1.1.1.9 14.66
-
 pH 7.0, 30°C, recombinant enzyme with xylitol and NAD+ as substrates Pichia kudriavzevii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.9 xylitol + NAD+
-
 Scheffersomyces stipitis D-xylulose + NADH + H+
-
 r
1.1.1.9 xylitol + NAD+
-
 Pichia kudriavzevii D-xylulose + NADH + H+
-
 r
1.1.1.9 xylitol + NAD+
-
 Torulaspora delbrueckii D-xylulose + NADH + H+
-
 r
1.1.1.9 xylitol + NAD+
-
 Pichia kudriavzevii QLB_09 D-xylulose + NADH + H+
-
 r
1.1.1.9 xylitol + NAD+
-
 Scheffersomyces stipitis NRRL Y-11545 D-xylulose + NADH + H+
-
 r
1.1.1.9 xylitol + NAD+
-
 Scheffersomyces stipitis NBRC 10063 D-xylulose + NADH + H+
-
 r
1.1.1.9 xylitol + NAD+
-
 Scheffersomyces stipitis ATCC 58785 D-xylulose + NADH + H+
-
 r
1.1.1.9 xylitol + NAD+
-
 Torulaspora delbrueckii BLQ_03 D-xylulose + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.9 ? x * 38000, SDS-PAGE Torulaspora delbrueckii
1.1.1.9 ? x * 38500, SDS-PAGE Scheffersomyces stipitis
1.1.1.9 ? x * 40100, SDS-PAGE Pichia kudriavzevii

Synonyms

EC Number Synonyms Comment Organism
1.1.1.9 IoXyl2p
-
 Pichia kudriavzevii
1.1.1.9 SsXyl2p
-
 Scheffersomyces stipitis
1.1.1.9 TdXyl2p
-
 Torulaspora delbrueckii
1.1.1.9 XDH
-
 Scheffersomyces stipitis
1.1.1.9 XDH
-
 Pichia kudriavzevii
1.1.1.9 XDH
-
 Torulaspora delbrueckii
1.1.1.9 XYL2
-
 Scheffersomyces stipitis
1.1.1.9 XYL2
-
 Pichia kudriavzevii
1.1.1.9 XYL2
-
 Torulaspora delbrueckii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.9 35
-
 oxidation of xylitol Scheffersomyces stipitis
1.1.1.9 35
-
 oxidation of xylitol Torulaspora delbrueckii
1.1.1.9 45
-
 oxidation of xylitol Pichia kudriavzevii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.9 20 55 over 40% of maximal activity within this range, profile overview Scheffersomyces stipitis
1.1.1.9 25 60 over 40% of maximal activity within this range, profile overview Pichia kudriavzevii
1.1.1.9 25 55 over 40% of maximal activity within this range, profile overview Torulaspora delbrueckii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.9 13.28
-
 xylitol pH 7.0, 30°C, recombinant enzyme Torulaspora delbrueckii
1.1.1.9 15.015
-
 xylitol pH 7.0, 30°C, recombinant enzyme Pichia kudriavzevii
1.1.1.9 19.62
-
 xylitol pH 7.0, 30°C, recombinant enzyme Scheffersomyces stipitis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.9 7.5
-
 oxidation of xylitol Scheffersomyces stipitis
1.1.1.9 8
-
 oxidation of xylitol Pichia kudriavzevii
1.1.1.9 8.5
-
 oxidation of xylitol Torulaspora delbrueckii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.9 5.5 9 over 50% of maximal activity within this range, profile overview Scheffersomyces stipitis
1.1.1.9 6 9 over 50% of maximal activity within this range, profile overview Pichia kudriavzevii
1.1.1.9 6.5 9 over 50% of maximal activity within this range, profile overview Torulaspora delbrueckii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.9 NAD+ strictly dependent on Scheffersomyces stipitis
1.1.1.9 NAD+ strictly dependent on, residues Val180, Asp200, and Lys205 are responsible for NAD+ binding. Especially, Asp200 in TdXyl2p determines the specificity for NAD+, which forms double-hydrogen bonds to the hydroxyl groups in the ribosyl moiety of NAD+ Torulaspora delbrueckii
1.1.1.9 NAD+ strictly dependent on, residues Val192, Asp212, and Lys217 are responsible for NAD+ binding. Especially, Asp212 in IoXyl2p determines the specificity for NAD+, which forms double-hydrogen bonds to the hydroxyl groups in the ribosyl moiety of NAD+ Pichia kudriavzevii
1.1.1.9 NADH
-
 Scheffersomyces stipitis
1.1.1.9 NADH
-
 Pichia kudriavzevii
1.1.1.9 NADH
-
 Torulaspora delbrueckii

General Information

EC Number General Information Comment Organism
1.1.1.9 additional information enzyme IoXyl2p ahs the conserved domain GxGxxG (Gly188-Gly190-Gly193 in IoXyl2p) for cofactor binding Pichia kudriavzevii
1.1.1.9 additional information enzyme TdXyl2p has the conserved domain GxGxxG (Gly176-Gly178-Gly181 in TdXyl2p) for cofactor binding Torulaspora delbrueckii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.9 1.024
-
 xylitol pH 7.0, 30°C, recombinant enzyme Pichia kudriavzevii
1.1.1.9 1.11
-
 xylitol pH 7.0, 30°C, recombinant enzyme Scheffersomyces stipitis
1.1.1.9 1.24
-
 xylitol pH 7.0, 30°C, recombinant enzyme Torulaspora delbrueckii