Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Castro-Fernandez, V.; Herrera-Morande, A.; Zamora, R.; Merino, F.; Gonzalez-Ordenes, F.; Padilla-Salinas, F.; Pereira, H.M.; Brandao-Neto, J.; Garratt, R.C.; Guixe, V.
    Reconstructed ancestral enzymes reveal that negative selection drove the evolution of substrate specificity in ADP-dependent kinases (2017), J. Biol. Chem., 292, 15598-15610 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.146 gene pfkC, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Pyrococcus horikoshii
2.7.1.146 gene pfkC, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Methanocaldococcus jannaschii
2.7.1.146 phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) uncultured bacterium
2.7.1.147 gene glkA, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Thermococcus litoralis
2.7.1.147 gene pfkC, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Methanocaldococcus jannaschii
2.7.1.147 phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) uncultured bacterium

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.146 purified enzyme mutant E72A, hanging drop vapor diffusion method, mixing of 0.002 ml of 8 mg/ml protein in 25 mM HEPES/NaOH, pH 7.8, 30 mM MgCl2, 20 mM fructose 6-phosphate, and 20 mM AMP with 0.002 ml of reservoir solution containing 18% PEG 3350 and 0.15 M NaI, 3 days at 18°C, X-ray diffraction structure determination and analysis at 2.61 A resolution, molecular replacement using the structure of Pyrococcus horikoshii ADP-PFK (PDB ID 3DRW) split into small and large domains as search models uncultured bacterium
2.7.1.147 purified enzyme mutant E72A, hanging drop vapor diffusion method, mixing of 0.002 ml of 8 mg/ml protein in 25 mM HEPES/NaOH, pH 7.8, 30 mM MgCl2, 20 mM fructose 6-phosphate, and 20 mM AMP with 0.002 ml of reservoir solution containing 18% PEG 3350 and 0.15 M NaI, 3 days at 18°C, X-ray diffraction structure determination and analysis at 2.61 A resolution, molecular replacement using the structure of Pyrococcus horikoshii ADP-PFK (PDB ID 3DRW) split into small and large domains as search models uncultured bacterium

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.146 E72A site-directed mutagenesis, the mutant shows reduced glucokinase and phosphofructokinase activities compared to wild-type enzyme uncultured bacterium
2.7.1.147 E72A site-directed mutagenesis, the mutant shows reduced glucokinase and phosphofructokinase activities compared to wild-type enzyme uncultured bacterium

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.146 additional information enzyme PhPFK is not significantly inhibited by D-glucose Pyrococcus horikoshii
2.7.1.147 D-glucose
-
 Methanocaldococcus jannaschii
2.7.1.147 D-glucose
-
 Thermococcus litoralis
2.7.1.147 additional information enzyme TlGK is not significantly inhibited by D-fructose 6-phosphate Thermococcus litoralis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.146 0.0063
-
 D-fructose 6-phosphate pH 6.5, 40°C Methanocaldococcus jannaschii
2.7.1.146 0.056
-
 D-fructose 6-phosphate pH 6.5, 40°C Pyrococcus horikoshii
2.7.1.146 0.119
-
 D-fructose 6-phosphate pH 6.5, 40°C, wild-type enzyme uncultured bacterium
2.7.1.146 2.8
-
 D-fructose 6-phosphate pH 6.5, 40°C, mutant E72A uncultured bacterium
2.7.1.147 0.0011
-
 D-glucose pH 7.8, 40°C Methanocaldococcus jannaschii
2.7.1.147 0.004
-
 D-glucose pH 7.8, 40°C, wild-type enzyme uncultured bacterium
2.7.1.147 0.044
-
 D-glucose pH 7.8, 40°C Thermococcus litoralis
2.7.1.147 1
-
 D-glucose pH 7.8, 40°C, mutant E72A uncultured bacterium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.146 Mg2+ required Pyrococcus horikoshii
2.7.1.146 Mg2+ required Methanocaldococcus jannaschii
2.7.1.147 Mg2+ required uncultured bacterium
2.7.1.147 Mg2+ required Thermococcus litoralis
2.7.1.147 Mg2+ required Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.146 ADP + D-fructose 6-phosphate uncultured bacterium
-
 AMP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.146 ADP + D-fructose 6-phosphate Pyrococcus horikoshii
-
 AMP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.146 ADP + D-fructose 6-phosphate Methanocaldococcus jannaschii
-
 AMP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.146 ADP + D-fructose 6-phosphate Methanocaldococcus jannaschii ATCC 43067
-
 AMP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.146 ADP + D-fructose 6-phosphate Pyrococcus horikoshii ATCC 700860
-
 AMP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.147 ADP + D-glucose uncultured bacterium
-
 AMP + D-glucose 6-phosphate
-
 ?
2.7.1.147 ADP + D-glucose Thermococcus litoralis
-
 AMP + D-glucose 6-phosphate
-
 ?
2.7.1.147 ADP + D-glucose Methanocaldococcus jannaschii
-
 AMP + D-glucose 6-phosphate
-
 ?
2.7.1.147 ADP + D-glucose Methanocaldococcus jannaschii ATCC 43067
-
 AMP + D-glucose 6-phosphate
-
 ?
2.7.1.147 ADP + D-glucose Thermococcus litoralis ATCC 51850
-
 AMP + D-glucose 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.146 Methanocaldococcus jannaschii Q58999
-
-
2.7.1.146 Pyrococcus horikoshii O59355
-
-
2.7.1.146 Pyrococcus horikoshii ATCC 700860 O59355
-
-
2.7.1.146 uncultured bacterium
-
 synthetic construct
-
2.7.1.147 Methanocaldococcus jannaschii Q58999
-
-
2.7.1.147 Methanocaldococcus jannaschii ATCC 43067 Q58999
-
-
2.7.1.147 Thermococcus litoralis Q7M537
-
-
2.7.1.147 Thermococcus litoralis ATCC 51850 Q7M537
-
-
2.7.1.147 uncultured bacterium
-
 synthetic construct
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.146 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and anion exchange chromatography uncultured bacterium
2.7.1.146 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and anion exchange chromatography Pyrococcus horikoshii
2.7.1.146 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and anion exchange chromatography Methanocaldococcus jannaschii
2.7.1.147 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and anion exchange chromatography uncultured bacterium
2.7.1.147 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and anion exchange chromatography Thermococcus litoralis
2.7.1.147 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and anion exchange chromatography Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.146 ADP + D-fructose 6-phosphate
-
 uncultured bacterium AMP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.146 ADP + D-fructose 6-phosphate
-
 Pyrococcus horikoshii AMP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.146 ADP + D-fructose 6-phosphate
-
 Methanocaldococcus jannaschii AMP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.146 ADP + D-fructose 6-phosphate
-
 Methanocaldococcus jannaschii ATCC 43067 AMP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.146 ADP + D-fructose 6-phosphate
-
 Pyrococcus horikoshii ATCC 700860 AMP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.146 additional information the enzyme also shows glucokinase activity, a bifunctional AncPFK/GK ancestor enzyme uncultured bacterium ?
-
-
2.7.1.146 additional information the enzyme from Methanococcus jannaschii also shows glucokinase activity, a bifunctional MjPFK/GK Methanocaldococcus jannaschii ?
-
-
2.7.1.146 additional information the PFK from Pyrococcus horikoshii (PhPFK) has no activity with glucose Pyrococcus horikoshii ?
-
-
2.7.1.146 additional information the enzyme from Methanococcus jannaschii also shows glucokinase activity, a bifunctional MjPFK/GK Methanocaldococcus jannaschii ATCC 43067 ?
-
-
2.7.1.146 additional information the PFK from Pyrococcus horikoshii (PhPFK) has no activity with glucose Pyrococcus horikoshii ATCC 700860 ?
-
-
2.7.1.147 ADP + D-glucose
-
 uncultured bacterium AMP + D-glucose 6-phosphate
-
 ?
2.7.1.147 ADP + D-glucose
-
 Thermococcus litoralis AMP + D-glucose 6-phosphate
-
 ?
2.7.1.147 ADP + D-glucose
-
 Methanocaldococcus jannaschii AMP + D-glucose 6-phosphate
-
 ?
2.7.1.147 ADP + D-glucose
-
 Methanocaldococcus jannaschii ATCC 43067 AMP + D-glucose 6-phosphate
-
 ?
2.7.1.147 ADP + D-glucose
-
 Thermococcus litoralis ATCC 51850 AMP + D-glucose 6-phosphate
-
 ?
2.7.1.147 additional information the enzyme also shows phosphofructokinase activity, a bifunctional AncPFK/GK ancestor enzyme uncultured bacterium ?
-
-
2.7.1.147 additional information the enzyme from Methanococcus jannaschii also shows phosphofructokinase activity, a bifunctional MjPFK/GK Methanocaldococcus jannaschii ?
-
-
2.7.1.147 additional information the glucokinase from Thermococcus litoralis shows no activity with fructose 6-phosphate Thermococcus litoralis ?
-
-
2.7.1.147 additional information the enzyme from Methanococcus jannaschii also shows phosphofructokinase activity, a bifunctional MjPFK/GK Methanocaldococcus jannaschii ATCC 43067 ?
-
-
2.7.1.147 additional information the glucokinase from Thermococcus litoralis shows no activity with fructose 6-phosphate Thermococcus litoralis ATCC 51850 ?
-
-

Synonyms

EC Number Synonyms Comment Organism
2.7.1.146 ADP-dependent glucokinase
-
 uncultured bacterium
2.7.1.146 ADP-GK
-
 uncultured bacterium
2.7.1.146 ancGK/PFK
-
 uncultured bacterium
2.7.1.146 MjPFK/GK
-
 Methanocaldococcus jannaschii
2.7.1.146 More see also EC 2.7.1.147 uncultured bacterium
2.7.1.146 More see also EC 2.7.1.147 Methanocaldococcus jannaschii
2.7.1.146 PFK
-
 Pyrococcus horikoshii
2.7.1.146 PhPFK
-
 Pyrococcus horikoshii
2.7.1.147 ADP-dependent glucokinase
-
 uncultured bacterium
2.7.1.147 ADP-dependent glucokinase
-
 Thermococcus litoralis
2.7.1.147 ADP-GK
-
 uncultured bacterium
2.7.1.147 ADP-GK
-
 Thermococcus litoralis
2.7.1.147 ancGK/PFK
-
 uncultured bacterium
2.7.1.147 MjPFK/GK
-
 Methanocaldococcus jannaschii
2.7.1.147 More see also EC 2.7.1.146 uncultured bacterium
2.7.1.147 More see also EC 2.7.1.146 Methanocaldococcus jannaschii
2.7.1.147 tlGK
-
 Thermococcus litoralis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.146 40
-
 assay at uncultured bacterium
2.7.1.146 40
-
 assay at Pyrococcus horikoshii
2.7.1.146 40
-
 assay at Methanocaldococcus jannaschii
2.7.1.147 40
-
 assay at uncultured bacterium
2.7.1.147 40
-
 assay at Thermococcus litoralis
2.7.1.147 40
-
 assay at Methanocaldococcus jannaschii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.146 0.33
-
 D-fructose 6-phosphate pH 6.5, 40°C, wild-type enzyme uncultured bacterium
2.7.1.146 0.38
-
 D-fructose 6-phosphate pH 6.5, 40°C, mutant E72A uncultured bacterium
2.7.1.146 2.7
-
 D-fructose 6-phosphate pH 6.5, 40°C Methanocaldococcus jannaschii
2.7.1.146 21
-
 D-fructose 6-phosphate pH 6.5, 40°C Pyrococcus horikoshii
2.7.1.147 0.24
-
 D-glucose pH 7.8, 40°C, wild-type enzyme uncultured bacterium
2.7.1.147 0.3
-
 D-glucose pH 7.8, 40°C, mutant E72A uncultured bacterium
2.7.1.147 5
-
 D-glucose pH 7.8, 40°C Methanocaldococcus jannaschii
2.7.1.147 42
-
 D-glucose pH 7.8, 40°C Thermococcus litoralis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.146 6.5
-
 assay at uncultured bacterium
2.7.1.146 6.5
-
 assay at Pyrococcus horikoshii
2.7.1.146 6.5
-
 assay at Methanocaldococcus jannaschii
2.7.1.147 7.8
-
 assay at uncultured bacterium
2.7.1.147 7.8
-
 assay at Thermococcus litoralis
2.7.1.147 7.8
-
 assay at Methanocaldococcus jannaschii

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.146 ADP
-
 Pyrococcus horikoshii
2.7.1.146 ADP
-
 Methanocaldococcus jannaschii
2.7.1.147 ADP
-
 Methanocaldococcus jannaschii
2.7.1.147 ADP dependent on uncultured bacterium
2.7.1.147 ADP dependent on Thermococcus litoralis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.147 14
-
 D-glucose pH 7.8, 40°C Thermococcus litoralis
2.7.1.147 97
-
 D-glucose pH 7.8, 40°C Methanocaldococcus jannaschii

General Information

EC Number General Information Comment Organism
2.7.1.146 evolution consensus phylogenetic tree of the ADP-dependent sugar kinases family, evolutionary history of enzyme substrate affinity, reconstruction uncultured bacterium
2.7.1.146 evolution consensus phylogenetic tree of the ADP-dependent sugar kinases family, evolutionary history of enzyme substrate affinity, reconstruction Pyrococcus horikoshii
2.7.1.146 evolution consensus phylogenetic tree of the ADP-dependent sugar kinases family, evolutionary history of enzyme substrate affinity, reconstruction Methanocaldococcus jannaschii
2.7.1.146 additional information molecular modeling, docking with D-glucose and D-fructose 6-phosphate, and molecular dynamics uncultured bacterium
2.7.1.146 additional information molecular modeling, docking with D-glucose and D-fructose 6-phosphate, and molecular dynamics Pyrococcus horikoshii
2.7.1.146 additional information molecular modeling, docking with D-glucose and D-fructose 6-phosphate, and molecular dynamics Methanocaldococcus jannaschii
2.7.1.147 evolution consensus phylogenetic tree of the ADP-dependent sugar kinases family, evolutionary history of enzyme substrate affinity, reconstruction uncultured bacterium
2.7.1.147 evolution consensus phylogenetic tree of the ADP-dependent sugar kinases family, evolutionary history of enzyme substrate affinity, reconstruction Thermococcus litoralis
2.7.1.147 evolution consensus phylogenetic tree of the ADP-dependent sugar kinases family, evolutionary history of enzyme substrate affinity, reconstruction Methanocaldococcus jannaschii
2.7.1.147 additional information molecular modeling, docking with D-glucose and D-fructose 6-phosphate, and molecular dynamics uncultured bacterium
2.7.1.147 additional information molecular modeling, docking with D-glucose and D-fructose 6-phosphate, and molecular dynamics Thermococcus litoralis
2.7.1.147 additional information molecular modeling, docking with D-glucose and D-fructose 6-phosphate, and molecular dynamics Methanocaldococcus jannaschii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.146 0.136
-
 D-fructose 6-phosphate pH 6.5, 40°C, mutant E72A uncultured bacterium
2.7.1.146 2.77
-
 D-fructose 6-phosphate pH 6.5, 40°C, wild-type enzyme uncultured bacterium
2.7.1.146 375
-
 D-fructose 6-phosphate pH 6.5, 40°C Pyrococcus horikoshii
2.7.1.146 428.6
-
 D-fructose 6-phosphate pH 6.5, 40°C Methanocaldococcus jannaschii
2.7.1.147 60
-
 D-glucose pH 7.8, 40°C, wild-type enzyme uncultured bacterium
2.7.1.147 81
-
 D-glucose pH 7.8, 40°C, mutant E72A uncultured bacterium
2.7.1.147 954.54
-
 D-glucose pH 7.8, 40°C Thermococcus litoralis
2.7.1.147 4545.45
-
 D-glucose pH 7.8, 40°C Methanocaldococcus jannaschii