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Literature summary extracted from

  • Liu, W.; Rogge, C.E.; Bambai, B.; Palmer, G.; Tsai, A.L.; Kulmacz, R.J.
    Characterization of the heme environment in Arabidopsis thaliana fatty acid alpha-dioxygenase-1 (2004), J. Biol. Chem., 279, 29805-29815 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.92 expression in Escherichia coli and insect cells Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.92 H163C mutation results in perturbation of the heme absorbance spectrum and oxygenase activity Arabidopsis thaliana
1.13.11.92 H163E mutation results in perturbation of the heme absorbance spectrum and oxygenase activity Arabidopsis thaliana
1.13.11.92 H163M mutation results in perturbation of the heme absorbance spectrum and oxygenase activity Arabidopsis thaliana
1.13.11.92 H163Y mutation results in perturbation of the heme absorbance spectrum and oxygenase activity Arabidopsis thaliana
1.13.11.92 H389C mutation results in perturbation of the heme absorbance spectrum and oxygenase activity Arabidopsis thaliana
1.13.11.92 H389E mutation results in perturbation of the heme absorbance spectrum and oxygenase activity Arabidopsis thaliana
1.13.11.92 H389M mutation results in perturbation of the heme absorbance spectrum and oxygenase activity Arabidopsis thaliana
1.13.11.92 H389Y mutation results in perturbation of the heme absorbance spectrum and oxygenase activity Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.92 cyanide
-
 Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.92 0.014
-
 oleic acid pH 7.8, 30°C Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.92 Arabidopsis thaliana A0A178V8W6 isoform PADOX-1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.92 recombinant protein Arabidopsis thaliana

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.92 11
-
 pH 7.8, 30°C Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.92 linoleic acid + O2
-
 Arabidopsis thaliana (2R)-2-hydroperoxy-(9Z,12Z)-octadeca-9,12-dienoic acid
-
 ?
1.13.11.92 linolenic acid + O2
-
 Arabidopsis thaliana (2R)-2-hydroperoxy-(9Z,12Z,15Z)-octadeca-9,12,15-trienoic acid
-
 ?
1.13.11.92 myristic acid + O2
-
 Arabidopsis thaliana (2R)-2-hydroperoxy-tetradecanoic acid
-
 ?
1.13.11.92 oleic acid + O2
-
 Arabidopsis thaliana (2R)-2-hydroperoxy-(9Z)-octadeca-9-enoic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.92 ? x * 98000, SDS-PAGE, GST-tagged recombinant protein Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
1.13.11.92 AXX17_At3g00510
-
 Arabidopsis thaliana
1.13.11.92 PADOX-1
-
 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.13.11.92 heme 1 mol of heme bound tightly but noncovalently per protein monomer. The high spin ferric heme can be reduced to the ferrous state by dithionite Arabidopsis thaliana

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.13.11.92 0.005
-
 cyanide pH 7.8, 30°C Arabidopsis thaliana