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Literature summary extracted from

  • Gonul Baltaci, N.; Guler, C.; Ceylan, H.; Kalin, S.N.; Adem, S.; Kocpinar, E.F.; Erdogan, O.; Budak, H.
    In vitro and in vivo effects of iron on the expression and activity of glucose 6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, and glutathione reductase in rat spleen (2018), J. Biochem. Mol. Toxicol., 2018, e22229 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.44 Fe3+ noncompetitive inhibition with respect to NADP+ and competitive inhibition with respect to 6-phospho-D-gluconate Rattus norvegicus
1.1.1.49 Fe3+ competitive inhibitor against the G6PD enzyme with respect to NADP+ and D-glucose 6-phosphate Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.1.7 Fe3+ stimulates, AC50 value: 0.327 mM. Enzymatic activity in the presence of nontoxic iron (0.87 and 3 ppm) is activated, no changes are seen in the presence of toxic iron concentrations (30 and 300 ppm) Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.44 120000
-
 gel filtration Rattus norvegicus
1.1.1.49 130000
-
 gel filtration Rattus norvegicus
1.8.1.7 126000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.44 6-phospho-D-gluconate + NADP+ Rattus norvegicus
-
 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.44 Rattus norvegicus P85968
-
-
1.1.1.49 Rattus norvegicus P05370
-
-
1.8.1.7 Rattus norvegicus F1LRE1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.44
-
 Rattus norvegicus
1.1.1.49
-
 Rattus norvegicus
1.8.1.7
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.44 spleen
-
 Rattus norvegicus
-
1.1.1.49 spleen
-
 Rattus norvegicus
-
1.8.1.7 spleen
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.44 15
-
 pH 7.6, and temperature not specified in the publication Rattus norvegicus
1.1.1.49 33.6
-
 pH 7.6, and temperature not specified in the publication Rattus norvegicus
1.8.1.7 51.25
-
 pH 7.6, and temperature not specified in the publication Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.44 6-phospho-D-gluconate + NADP+
-
 Rattus norvegicus D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 ?
1.1.1.49 D-glucose 6-phosphate + NADP+
-
 Rattus norvegicus 6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.44 dimer 2 * 59000, SDS-PAGE Rattus norvegicus
1.1.1.49 dimer 2 * 63000, SDS-PAGE Rattus norvegicus
1.8.1.7 dimer 2 * 62000, SDS-PAGE Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.44 6-phosphogluconate dehydrogenase
-
 Rattus norvegicus
1.1.1.44 6PGD
-
 Rattus norvegicus
1.1.1.49 G6PD
-
 Rattus norvegicus
1.1.1.49 glucose 6-phosphate dehydrogenase
-
 Rattus norvegicus
1.8.1.7 glutathione reductase
-
 Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.44 0.186
-
 Fe3+ pH 7.6, and temperature not specified in the publication, noncompetitive inhibition with respect to NADP+ Rattus norvegicus
1.1.1.44 0.223
-
 Fe3+ pH 7.6, and temperature not specified in the publication, competitive inhibition with respect to 6-phospho-D-gluconate Rattus norvegicus
1.1.1.49 0.368
-
 Fe3+ pH 7.6, and temperature not specified in the publication, competitive inhibition with respect to NADP+ Rattus norvegicus
1.1.1.49 0.388
-
 Fe3+ pH 7.6, and temperature not specified in the publication, competitive inhibition with respect to D-glucose 6-phosphate Rattus norvegicus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.1.1.44 0.225
-
 pH 7.6, and temperature not specified in the publication Rattus norvegicus Fe3+
1.1.1.49 0.346
-
 pH 7.6, and temperature not specified in the publication Rattus norvegicus Fe3+

Expression

EC Number Organism Comment Expression
1.1.1.44 Rattus norvegicus gene expressions is significantly repressed in the presence of nontoxic (0.87 and 3 ppm) and toxic iron concentrations (30 and 300 ppm) down
1.1.1.49 Rattus norvegicus gene expressions is significantly repressed in the presence of nontoxic (0.87 and 3 ppm) and toxic iron concentrations (30 and 300 ppm) down
1.8.1.7 Rattus norvegicus gene expressions is significantly repressed in the presence of nontoxic (0.87 and 3 ppm) and toxic iron concentrations (30 and 300 ppm) down