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Literature summary extracted from

  • Rudat, A.K.; Pokhrel, A.; Green, T.J.; Gray, M.J.
    Mutations in Escherichia coli polyphosphate kinase that lead to dramatically increased in vivo polyphosphate levels (2018), J. Bacteriol., 200, e00697-17 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.7.4.1 environmental protection E245K mutation leads to very high polyphosphate accumulation in vivo but is not different from the wild type in either activity or chain length of polyphosphate produced in vitro. Polyphosphate accumulation by bacteria is important in biotechnology applications, e.g. to enhanced biological phosphate removal (EBPR) from wastewater Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.7.4.1 D230N the variant has somewhat greater processivity than the wild-type enzyme Escherichia coli
2.7.4.1 E173K the variant has somewhat greater processivity than the wild-type enzyme Escherichia coli
2.7.4.1 E245K mutation leads to very high polyP accumulation in vivo but is not different from the wild type in either activity or chain length of polyP produced in vitro Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.1 ADP
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.1 2.06
-
 ATP pH 7.5, 37°C, mutant enzyme D230N Escherichia coli
2.7.4.1 2.07
-
 ATP pH 7.5, 37°C, wild-type enzyme Escherichia coli
2.7.4.1 2.08
-
 ATP pH 7.5, 37°C, mutant enzyme E245K Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.1 ATP + (phosphate)n Escherichia coli
-
 ADP + (phosphate)n+1
-
 ?
2.7.4.1 ATP + (phosphate)n Escherichia coli K12
-
 ADP + (phosphate)n+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.1 Escherichia coli P0A7B1
-
-
2.7.4.1 Escherichia coli K12 P0A7B1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.1 ATP + (phosphate)n
-
 Escherichia coli ADP + (phosphate)n+1
-
 ?
2.7.4.1 ATP + (phosphate)n
-
 Escherichia coli K12 ADP + (phosphate)n+1
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.4.1 polyP kinase
-
 Escherichia coli
2.7.4.1 PPK
-
 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.4.1 0.135
-
 ADP pH 7.5, 37°C, mutant enzyme D230N Escherichia coli
2.7.4.1 0.144
-
 ADP pH 7.5, 37°C, mutant enzyme E245K Escherichia coli
2.7.4.1 0.156
-
 ADP pH 7.5, 37°C, wild-type enzyme Escherichia coli

General Information

EC Number General Information Comment Organism
2.7.4.1 malfunction a mutant lacking the enzyme (PPK) makes no detectable polyP under any condition Escherichia coli