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Literature summary extracted from

  • Harris, S.C.; Devendran, S.; Mendez-Garcia, C.; Mythen, S.M.; Wright, C.L.; Fields, C.J.; Hernandez, A.G.; Cann, I.; Hylemon, P.B.; Ridlon, J.M.
    Bile acid oxidation by Eggerthella lenta strains C592 and DSM 2243T (2018), Gut Microbes, 9, 523-539 .
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.176 H2 H2 strongly inhibits bile acid oxidation by the enzyme Eggerthella lenta

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.176 0.09678
-
 deoxycholic acid with NAD+ as cosubstrate, pH and temperature not specified in the publication Eggerthella lenta
1.1.1.176 0.19162
-
 12-oxolithocholic acid with NADH as cosubstrate, pH and temperature not specified in the publication Eggerthella lenta

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.176 12-oxolithocholic acid + NADH + H+ Eggerthella lenta
-
 deoxycholic acid + NAD+
-
 r
1.1.1.176 12-oxolithocholic acid + NADH + H+ Eggerthella lenta C592
-
 deoxycholic acid + NAD+
-
 r
1.1.1.176 12-oxolithocholic acid + NADH + H+ Eggerthella lenta DSM 2243T
-
 deoxycholic acid + NAD+
-
 r
1.1.1.176 12-oxolithocholic acid + NADPH + H+ Eggerthella lenta
-
 deoxycholic acid + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.176 Eggerthella lenta
-
-
-
1.1.1.176 Eggerthella lenta C592
-
-
-
1.1.1.176 Eggerthella lenta DSM 2243T
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.176 Talon metal affinity column chromatography Eggerthella lenta

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.176 12-oxolithocholic acid + NADH + H+
-
 Eggerthella lenta deoxycholic acid + NAD+
-
 r
1.1.1.176 12-oxolithocholic acid + NADH + H+ 19.6% activity compared to deoxycholic acid Eggerthella lenta deoxycholic acid + NAD+
-
 ?
1.1.1.176 12-oxolithocholic acid + NADH + H+
-
 Eggerthella lenta C592 deoxycholic acid + NAD+
-
 r
1.1.1.176 12-oxolithocholic acid + NADH + H+ 19.6% activity compared to deoxycholic acid Eggerthella lenta C592 deoxycholic acid + NAD+
-
 ?
1.1.1.176 12-oxolithocholic acid + NADH + H+
-
 Eggerthella lenta DSM 2243T deoxycholic acid + NAD+
-
 r
1.1.1.176 12-oxolithocholic acid + NADH + H+ 19.6% activity compared to deoxycholic acid Eggerthella lenta DSM 2243T deoxycholic acid + NAD+
-
 ?
1.1.1.176 12-oxolithocholic acid + NADPH + H+
-
 Eggerthella lenta deoxycholic acid + NADP+
-
 r
1.1.1.176 cholic acid + NAD+ 98.5% activity compared to deoxycholic acid Eggerthella lenta 3-oxocholic acid + NADH + H+
-
 r
1.1.1.176 cholic acid + NAD+ 98.5% activity compared to deoxycholic acid Eggerthella lenta C592 3-oxocholic acid + NADH + H+
-
 r
1.1.1.176 cholic acid + NAD+ 98.5% activity compared to deoxycholic acid Eggerthella lenta DSM 2243T 3-oxocholic acid + NADH + H+
-
 r
1.1.1.176 deoxycholic acid + NAD+ 100% activity Eggerthella lenta 12-oxolithocholic acid + NADH + H+
-
 r
1.1.1.176 deoxycholic acid + NADP+ 3.14% activity compared to NAD+ Eggerthella lenta 12-oxolithocholic acid + NADH + H+
-
 r
1.1.1.176 glycocholic acid + NAD+ 81.08% activity compared to deoxycholic acid Eggerthella lenta 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoyl glycine + NADH
-
 r
1.1.1.176 glycocholic acid + NAD+ 81.08% activity compared to deoxycholic acid Eggerthella lenta C592 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoyl glycine + NADH
-
 r
1.1.1.176 glycocholic acid + NAD+ 81.08% activity compared to deoxycholic acid Eggerthella lenta DSM 2243T 3alpha,7alpha-dihydroxy-12-oxo-5beta-cholanoyl glycine + NADH
-
 r
1.1.1.176 glycodeoxycholic acid + NAD+ 97.8% activity compared to deoxycholic acid Eggerthella lenta ? + NADH + H+
-
 r
1.1.1.176 additional information no activity with chenodeoxycholic acid, glycochenodeoxycholic acid, taurochenodeoxycholic acid, 12-oxochenodeoxycholic acid, 3-oxodeoxycholic acid, and 3-oxocholic acid Eggerthella lenta ?
-
-
1.1.1.176 additional information no activity with chenodeoxycholic acid, glycochenodeoxycholic acid, taurochenodeoxycholic acid, 12-oxochenodeoxycholic acid, 3-oxodeoxycholic acid, and 3-oxocholic acid Eggerthella lenta C592 ?
-
-
1.1.1.176 additional information no activity with chenodeoxycholic acid, glycochenodeoxycholic acid, taurochenodeoxycholic acid, 12-oxochenodeoxycholic acid, 3-oxodeoxycholic acid, and 3-oxocholic acid Eggerthella lenta DSM 2243T ?
-
-
1.1.1.176 taurocholic acid + NAD+ 98.95% activity compared to deoxycholic acid Eggerthella lenta ? + NADH + H+
-
 r
1.1.1.176 taurodeoxycholic acid + NAD+ 90.84% activity compared to deoxycholic acid Eggerthella lenta ? + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.176 ? x * 27500, SDS-PAGE Eggerthella lenta
1.1.1.176 ? x * 27078, calculated from amino acid sequence Eggerthella lenta

Synonyms

EC Number Synonyms Comment Organism
1.1.1.176 12alpha-HSDH
-
 Eggerthella lenta
1.1.1.176 Elen_2515
-
 Eggerthella lenta

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.176 2.03
-
 12-oxolithocholic acid with NADH as cosubstrate, pH and temperature not specified in the publication Eggerthella lenta
1.1.1.176 28.085
-
 deoxycholic acid with NAD+ as cosubstrate, pH and temperature not specified in the publication Eggerthella lenta

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.176 7 7.5 pH optimum in the reductive direction with NAD+ as cofactor Eggerthella lenta
1.1.1.176 9
-
 pH optimum in the oxidative direction with NAD+ as cofactor Eggerthella lenta

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.176 NAD+ preferred cofactor Eggerthella lenta
1.1.1.176 NADP+
-
 Eggerthella lenta

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.176 10.59
-
 12-oxolithocholic acid with NADH as cosubstrate, pH and temperature not specified in the publication Eggerthella lenta
1.1.1.176 290.19
-
 deoxycholic acid with NAD+ as cosubstrate, pH and temperature not specified in the publication Eggerthella lenta