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Literature summary extracted from

  • Dongming, M.; Chong, X.; Fatima, A.-G.; Hong, W.; Jinfen, Y.; Rika, J.; De-Yu, X.
    Overexpression of Artemisia annua cinnamyl alcohol dehydrogenase increases lignin and coumarin and reduces artemisinin and other sesquiterpenes (2018), Front. Plant Sci., 9, 828 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.195 expressed in Escherichia coli BL21 cells Artemisia annua

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.195 0.00194
-
 sinapyl aldehyde at pH 7.5 and 30°C Artemisia annua
1.1.1.195 0.01076
-
 coniferyl aldehyde at pH 7.5 and 30°C Artemisia annua
1.1.1.195 0.01105
-
 4-coumaryl aldehyde at pH 7.5 and 30°C Artemisia annua

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.195 cytosol
-
 Artemisia annua 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.195 artemisinic aldehyde + NADPH + H+ Artemisia annua
-
 artemisinic alcohol + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.195 Artemisia annua A0A2Z5D8A5 sweet wormwood
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.195 Ni-NTA column chromatography Artemisia annua

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.195 leaf
-
 Artemisia annua
-
1.1.1.195 seedling
-
 Artemisia annua
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.195 4-coumaryl aldehyde + NADPH + H+
-
 Artemisia annua 4-coumaryl alcohol + NADP+
-
 ?
1.1.1.195 artemisinic aldehyde + NADPH + H+
-
 Artemisia annua artemisinic alcohol + NADP+
-
 ?
1.1.1.195 coniferyl aldehyde + NADPH + H+
-
 Artemisia annua coniferyl alcohol + NADP+
-
 ?
1.1.1.195 sinapyl aldehyde + NADPH + H+
-
 Artemisia annua sinapyl alcohol + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.195 CAD
-
 Artemisia annua
1.1.1.195 cinnamyl alcohol dehydrogenase
-
 Artemisia annua

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.195 12.36
-
 4-coumaryl aldehyde at pH 7.5 and 30°C Artemisia annua
1.1.1.195 13.82
-
 coniferyl aldehyde at pH 7.5 and 30°C Artemisia annua
1.1.1.195 14.03
-
 sinapyl aldehyde at pH 7.5 and 30°C Artemisia annua

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.195 NADPH
-
 Artemisia annua

General Information

EC Number General Information Comment Organism
1.1.1.195 malfunction enzyme downregulation or knockout causes a reduction of lignin, which leads to severe plant dwarfism and a decreased biomass as well as decreased plant resistance to pathogens Artemisia annua
1.1.1.195 metabolism key enzyme in the biosynthesis of lignin. Artemisinin, arteannuin B, and other sesquiterpenes are profiled in the leaves of enzyme-overexpressing vs. wild-type plants Artemisia annua

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.195 1120
-
 4-coumaryl aldehyde at pH 7.5 and 30°C Artemisia annua
1.1.1.195 1280
-
 coniferyl aldehyde at pH 7.5 and 30°C Artemisia annua
1.1.1.195 7230
-
 sinapyl aldehyde at pH 7.5 and 30°C Artemisia annua