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Literature summary extracted from

  • Zarei, A.; Brikis, C.J.; Bajwa, V.S.; Chiu, G.Z.; Simpson, J.P.; DeEll, J.R.; Bozzo, G.G.; Shelp, B.J.
    Plant glyoxylate/succinic semialdehyde reductases comparative biochemical properties, function during chilling stress, and subcellular localization (2017), Front. Plant Sci., 8, 1399 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.79 expressed in Escherichia coli BL21(DE3) cells Oryza sativa
1.1.1.79 expressed in Escherichia coli BL21(DE3) cells Arabidopsis thaliana
1.1.1.79 expressed in Escherichia coli BL21(DE3) cells Malus domestica

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.79 NADP+
-
 Arabidopsis thaliana
1.1.1.79 NADP+
-
 Malus domestica
1.1.1.79 NADP+
-
 Oryza sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.79 0.0012
-
 NADPH isoform GLYR2, with succinic semialdehyde as cosubstrate, at pH 7.8 and 25°C Arabidopsis thaliana
1.1.1.79 0.0014
-
 NADPH isoform GLYR2, with glyoxylate as cosubstrate, at pH 7.8 and 25°C Arabidopsis thaliana
1.1.1.79 0.0018
-
 NADPH isoform GLYR2, with glyoxylate as cosubstrate, at pH 7.3 and 25°C Malus domestica
1.1.1.79 0.0022
-
 NADPH isoform GLYR1, with glyoxylate as cosubstrate, at pH 7.8 and 25°C Arabidopsis thaliana
1.1.1.79 0.0026
-
 NADPH isoform GLYR1, with succinic semialdehyde as cosubstrate, at pH 7.8 and 25°C Arabidopsis thaliana
1.1.1.79 0.0033
-
 NADPH isoform GLYR1, with glyoxylate as cosubstrate, at pH 7.5 and 25°C Malus domestica
1.1.1.79 0.0074
-
 NADPH isoform GLYR1, with succinic semialdehyde as cosubstrate, at pH 7.5 and 25°C Malus domestica
1.1.1.79 0.0084
-
 NADPH isoform GLYR2, with glyoxylate as cosubstrate, at pH 7.1 and 25°C Oryza sativa
1.1.1.79 0.0088
-
 NADPH isoform GLYR1, with glyoxylate as cosubstrate, at pH 6.5 and 25°C Oryza sativa
1.1.1.79 0.0117
-
 NADPH isoform GLYR2, with succinic semialdehyde as cosubstrate, at pH 7.3 and 25°C Malus domestica
1.1.1.79 0.0125
-
 NADPH isoform GLYR2, with succinic semialdehyde as cosubstrate, at pH 7.1 and 25°C Oryza sativa
1.1.1.79 0.0141
-
 glyoxylate isoform GLYR1, at pH 7.5 and 25°C Malus domestica
1.1.1.79 0.0191
-
 glyoxylate isoform GLYR2, at pH 7.1 and 25°C Oryza sativa
1.1.1.79 0.0193
-
 glyoxylate isoform GLYR2, at pH 7.8 and 25°C Arabidopsis thaliana
1.1.1.79 0.0232
-
 glyoxylate isoform GLYR1, at pH 7.8 and 25°C Arabidopsis thaliana
1.1.1.79 0.0239
-
 glyoxylate isoform GLYR2, at pH 7.3 and 25°C Malus domestica
1.1.1.79 0.0362
-
 NADPH isoform GLYR1, with succinic semialdehyde as cosubstrate, at pH 6.5 and 25°C Oryza sativa
1.1.1.79 0.0532
-
 glyoxylate isoform GLYR1, at pH 6.5 and 25°C Oryza sativa
1.1.1.79 0.87
-
 Succinic semialdehyde isoform GLYR1, at pH 7.8 and 25°C Arabidopsis thaliana
1.1.1.79 1.133
-
 Succinic semialdehyde isoform GLYR1, at pH 7.5 and 25°C Malus domestica
1.1.1.79 1.457
-
 Succinic semialdehyde isoform GLYR2, at pH 7.1 and 25°C Oryza sativa
1.1.1.79 4.003
-
 Succinic semialdehyde isoform GLYR1, at pH 6.5 and 25°C Oryza sativa
1.1.1.79 6.5
-
 Succinic semialdehyde isoform GLYR2, at pH 7.3 and 25°C Malus domestica
1.1.1.79 8.96
-
 Succinic semialdehyde isoform GLYR2, at pH 7.8 and 25°C Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.79 cytosol isoform GLYR1 Oryza sativa 5829
-
1.1.1.79 cytosol isoform GLYR1 Arabidopsis thaliana 5829
-
1.1.1.79 cytosol isoform GLYR1 Malus domestica 5829
-
1.1.1.79 mitochondrion isoform GLYR2 Oryza sativa 5739
-
1.1.1.79 mitochondrion isoform GLYR2 Arabidopsis thaliana 5739
-
1.1.1.79 mitochondrion isoform GLYR2 Malus domestica 5739
-
1.1.1.79 plastid isoform GLYR2 Oryza sativa 9536
-
1.1.1.79 plastid isoform GLYR2 Arabidopsis thaliana 9536
-
1.1.1.79 plastid isoform GLYR2 Malus domestica 9536
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.79 glyoxylate + NADPH + H+ Oryza sativa
-
 glycolate + NADP+
-
 ?
1.1.1.79 glyoxylate + NADPH + H+ Arabidopsis thaliana
-
 glycolate + NADP+
-
 ?
1.1.1.79 glyoxylate + NADPH + H+ Malus domestica
-
 glycolate + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.79 Arabidopsis thaliana F4I907
-
-
1.1.1.79 Arabidopsis thaliana Q9LSV0
-
-
1.1.1.79 Malus domestica A0A1C8M582
-
-
1.1.1.79 Malus domestica A0A1C8M593
-
-
1.1.1.79 Oryza sativa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.79 nickel affinity column chromatography Oryza sativa
1.1.1.79 nickel affinity column chromatography Arabidopsis thaliana
1.1.1.79 nickel affinity column chromatography Malus domestica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.79 fruit
-
 Malus domestica
-
1.1.1.79 leaf
-
 Oryza sativa
-
1.1.1.79 leaf
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.79 glyoxylate + NADPH + H+
-
 Oryza sativa glycolate + NADP+
-
 ?
1.1.1.79 glyoxylate + NADPH + H+
-
 Arabidopsis thaliana glycolate + NADP+
-
 ?
1.1.1.79 glyoxylate + NADPH + H+
-
 Malus domestica glycolate + NADP+
-
 ?
1.1.1.79 glyoxylate + NADPH + H+ the enzyme prefers glyoxylate over succinic semialdehyde, and has a high affinity for their co-substrate NADPH Oryza sativa glycolate + NADP+
-
 ?
1.1.1.79 glyoxylate + NADPH + H+ the enzyme prefers glyoxylate over succinic semialdehyde, and has a high affinity for their co-substrate NADPH Arabidopsis thaliana glycolate + NADP+
-
 ?
1.1.1.79 glyoxylate + NADPH + H+ the enzyme prefers glyoxylate over succinic semialdehyde, and has a high affinity for their co-substrate NADPH Malus domestica glycolate + NADP+
-
 ?
1.1.1.79 succinic semialdehyde + NADPH + H+ the enzyme prefers glyoxylate over succinic semialdehyde, and has a high affinity for their co-substrate NADPH Oryza sativa 4-hydroxybutyrate + NADP+
-
 ?
1.1.1.79 succinic semialdehyde + NADPH + H+ the enzyme prefers glyoxylate over succinic semialdehyde, and has a high affinity for their co-substrate NADPH Arabidopsis thaliana 4-hydroxybutyrate + NADP+
-
 ?
1.1.1.79 succinic semialdehyde + NADPH + H+ the enzyme prefers glyoxylate over succinic semialdehyde, and has a high affinity for their co-substrate NADPH Malus domestica 4-hydroxybutyrate + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.79 ? x * 30500, isoform GLYR1, calculated from amino acid sequence Oryza sativa
1.1.1.79 ? x * 30700, isoform GLYR1, calculated from amino acid sequence Arabidopsis thaliana
1.1.1.79 ? x * 31400, isoform GLYR2, calculated from amino acid sequence Oryza sativa
1.1.1.79 ? x * 31800, isoform GLYR1, calculated from amino acid sequence Malus domestica
1.1.1.79 ? x * 33100, isoform GLYR2, calculated from amino acid sequence Malus domestica
1.1.1.79 ? x * 33200, isoform GLYR2, calculated from amino acid sequence Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
1.1.1.79 glyoxylate/succinic semialdehyde reductase
-
 Oryza sativa
1.1.1.79 glyoxylate/succinic semialdehyde reductase
-
 Arabidopsis thaliana
1.1.1.79 glyoxylate/succinic semialdehyde reductase
-
 Malus domestica
1.1.1.79 GLYR1 isoform Oryza sativa
1.1.1.79 GLYR1 isoform Arabidopsis thaliana
1.1.1.79 GLYR1 isoform Malus domestica
1.1.1.79 GLYR2 isoform Oryza sativa
1.1.1.79 GLYR2 isoform Arabidopsis thaliana
1.1.1.79 GLYR2 isoform Malus domestica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.79 4.6
-
 Succinic semialdehyde isoform GLYR1, at pH 7.5 and 25°C Malus domestica
1.1.1.79 5.5
-
 NADPH isoform GLYR1, with succinic semialdehyde as cosubstrate, at pH 7.5 and 25°C Malus domestica
1.1.1.79 6.5
-
 NADPH isoform GLYR2, with succinic semialdehyde as cosubstrate, at pH 7.1 and 25°C Oryza sativa
1.1.1.79 7.2
-
 Succinic semialdehyde isoform GLYR2, at pH 7.1 and 25°C Oryza sativa
1.1.1.79 8.1
-
 NADPH isoform GLYR1, with succinic semialdehyde as cosubstrate, at pH 7.8 and 25°C Arabidopsis thaliana
1.1.1.79 8.6
-
 NADPH isoform GLYR2, with succinic semialdehyde as cosubstrate, at pH 7.3 and 25°C Malus domestica
1.1.1.79 8.9
-
 NADPH isoform GLYR2, with glyoxylate as cosubstrate, at pH 7.1 and 25°C Oryza sativa
1.1.1.79 9.3
-
 NADPH isoform GLYR1, with glyoxylate as cosubstrate, at pH 7.8 and 25°C Arabidopsis thaliana
1.1.1.79 10.1
-
 Succinic semialdehyde isoform GLYR1, at pH 7.8 and 25°C Arabidopsis thaliana
1.1.1.79 10.3
-
 Succinic semialdehyde isoform GLYR2, at pH 7.3 and 25°C Malus domestica
1.1.1.79 10.7
-
 NADPH isoform GLYR1, with succinic semialdehyde as cosubstrate, at pH 6.5 and 25°C Oryza sativa
1.1.1.79 10.7
-
 NADPH isoform GLYR2, with succinic semialdehyde as cosubstrate, at pH 7.8 and 25°C Arabidopsis thaliana
1.1.1.79 10.8
-
 glyoxylate isoform GLYR2, at pH 7.1 and 25°C Oryza sativa
1.1.1.79 12
-
 NADPH isoform GLYR2, with glyoxylate as cosubstrate, at pH 7.8 and 25°C Arabidopsis thaliana
1.1.1.79 14.2
-
 NADPH isoform GLYR1, with glyoxylate as cosubstrate, at pH 6.5 and 25°C Oryza sativa
1.1.1.79 15.7
-
 NADPH isoform GLYR2, with glyoxylate as cosubstrate, at pH 7.3 and 25°C Malus domestica
1.1.1.79 17
-
 Succinic semialdehyde isoform GLYR2, at pH 7.8 and 25°C Arabidopsis thaliana
1.1.1.79 18.4
-
 glyoxylate isoform GLYR2, at pH 7.8 and 25°C Arabidopsis thaliana
1.1.1.79 19.1
-
 glyoxylate isoform GLYR2, at pH 7.3 and 25°C Malus domestica
1.1.1.79 21.9
-
 Succinic semialdehyde isoform GLYR1, at pH 6.5 and 25°C Oryza sativa
1.1.1.79 22.3
-
 NADPH isoform GLYR1, with glyoxylate as cosubstrate, at pH 7.5 and 25°C Malus domestica
1.1.1.79 28.4
-
 glyoxylate isoform GLYR1, at pH 7.8 and 25°C Arabidopsis thaliana
1.1.1.79 30.9
-
 glyoxylate isoform GLYR1, at pH 7.5 and 25°C Malus domestica
1.1.1.79 33.7
-
 glyoxylate isoform GLYR1, at pH 6.5 and 25°C Oryza sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.79 6
-
 isoform GLYR1 Oryza sativa
1.1.1.79 6.8
-
-
 Malus domestica
1.1.1.79 6.8
-
 isoform GLYR2 Oryza sativa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.79 5.5 7 enzyme isoform GLYR1 retains at least half of maximal activity over the pH range of 5.5-7.0 Oryza sativa
1.1.1.79 5.5 7 enzyme isoform GLYR1 retains at least half of maximal activity over the pH range of 5.5-7.0 Malus domestica

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.79 NADPH the highest catalytic efficiency is observed for NADPH Oryza sativa
1.1.1.79 NADPH the highest catalytic efficiency is observed for NADPH Arabidopsis thaliana
1.1.1.79 NADPH the highest catalytic efficiency is observed for NADPH Malus domestica

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.79 1.6
-
 Succinic semialdehyde isoform GLYR2, at pH 7.3 and 25°C Malus domestica
1.1.1.79 1.9
-
 Succinic semialdehyde isoform GLYR2, at pH 7.8 and 25°C Arabidopsis thaliana
1.1.1.79 5.1
-
 Succinic semialdehyde isoform GLYR1, at pH 7.5 and 25°C Malus domestica
1.1.1.79 5.1
-
 Succinic semialdehyde isoform GLYR2, at pH 7.1 and 25°C Oryza sativa
1.1.1.79 5.4
-
 Succinic semialdehyde isoform GLYR1, at pH 6.5 and 25°C Oryza sativa
1.1.1.79 11.6
-
 Succinic semialdehyde isoform GLYR1, at pH 7.8 and 25°C Arabidopsis thaliana
1.1.1.79 290
-
 NADPH isoform GLYR1, with succinic semialdehyde as cosubstrate, at pH 6.5 and 25°C Oryza sativa
1.1.1.79 349
-
 glyoxylate isoform GLYR2, at pH 7.1 and 25°C Oryza sativa
1.1.1.79 525
-
 NADPH isoform GLYR2, with succinic semialdehyde as cosubstrate, at pH 7.1 and 25°C Oryza sativa
1.1.1.79 636
-
 glyoxylate isoform GLYR1, at pH 6.5 and 25°C Oryza sativa
1.1.1.79 660
-
 NADPH isoform GLYR2, with glyoxylate as cosubstrate, at pH 7.8 and 25°C Arabidopsis thaliana
1.1.1.79 796
-
 glyoxylate isoform GLYR2, at pH 7.3 and 25°C Malus domestica
1.1.1.79 832
-
 NADPH isoform GLYR1, with succinic semialdehyde as cosubstrate, at pH 7.5 and 25°C Malus domestica
1.1.1.79 833
-
 NADPH isoform GLYR2, with succinic semialdehyde as cosubstrate, at pH 7.3 and 25°C Malus domestica
1.1.1.79 906
-
 glyoxylate isoform GLYR2, at pH 7.8 and 25°C Arabidopsis thaliana
1.1.1.79 1040
-
 NADPH isoform GLYR2, with glyoxylate as cosubstrate, at pH 7.1 and 25°C Oryza sativa
1.1.1.79 1259
-
 glyoxylate isoform GLYR1, at pH 7.8 and 25°C Arabidopsis thaliana
1.1.1.79 1729
-
 NADPH isoform GLYR1, with glyoxylate as cosubstrate, at pH 6.5 and 25°C Oryza sativa
1.1.1.79 2230
-
 glyoxylate isoform GLYR1, at pH 7.5 and 25°C Malus domestica
1.1.1.79 2870
-
 NADPH isoform GLYR1, with glyoxylate as cosubstrate, at pH 7.8 and 25°C Arabidopsis thaliana
1.1.1.79 3500
-
 NADPH isoform GLYR1, with succinic semialdehyde as cosubstrate, at pH 7.8 and 25°C Arabidopsis thaliana
1.1.1.79 6803
-
 NADPH isoform GLYR1, with glyoxylate as cosubstrate, at pH 7.5 and 25°C Malus domestica
1.1.1.79 9180
-
 NADPH isoform GLYR2, with succinic semialdehyde as cosubstrate, at pH 7.8 and 25°C Arabidopsis thaliana
1.1.1.79 10070
-
 NADPH isoform GLYR2, with glyoxylate as cosubstrate, at pH 7.3 and 25°C Malus domestica