Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Gonzalez-Ordenes, F.; Cea, P.A.; Fuentes-Ugarte, N.; Munoz, S.M.; Zamora, R.A.; Leonardo, D.; Garratt, R.C.; Castro-Fernandez, V.; Guixe, V.
    ADP-dependent kinases from the archaeal order Methanosarcinales adapt to salt by a non-canonical evolutionarily conserved strategy (2018), Front. Microbiol., 9, 1305 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.146 glycine betaine
-
 uncultured bacterium
2.7.1.146 glycine betaine activates at 0.1-1.5 M Methanosarcina mazei
2.7.1.147 glycine betaine
-
 uncultured bacterium
2.7.1.147 glycine betaine activates at 0.1-1.5 M Methanosarcina mazei

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.146 gene pfkC, phylogenetic analysis Methanohalobium evestigatum
2.7.1.146 gene pfkC, phylogenetic analysis Methanosarcina mazei
2.7.1.146 phylogenetic analysis uncultured bacterium
2.7.1.146 phylogenetic analysis Pyrococcus horikoshii
2.7.1.147 gene pfkC, phylogenetic analysis Methanohalobium evestigatum
2.7.1.147 gene pfkC, phylogenetic analysis Methanosarcina mazei
2.7.1.147 phylogenetic analysis uncultured bacterium

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.146 purified enzyme, sitting drop vapor diffusion method, mixing of 0.001 ml of 10 mg/ml protein, 2.5 mM ADP, 200 mM NaCl, 7.5 mM MgCl2, 25 mM HEPES, pH 7.8, and 1 mM 2-mercaptoethanol with 0.001 ml of reservoir solution containing 20 mM CdCl2, 20 mM MgCl2, 20 mM NiCl2, 24% PEG MME 2000, and 100 mM sodium acetate, pH 4.5, 2 weeks, 19°C, X-ray diffraction structure determination and analysis at 1.46 A resolution, molecular replacement using the structure of the ancestral ADP-dependent kinase (PDB ID 5K27) split into small and large domains as search models Methanohalobium evestigatum
2.7.1.146 purified enzyme, sitting drop vapor diffusion method, mixing of 0.001 ml of 10 mg/ml protein, 2.5 mM ADP, 200 mM NaCl, 7.5 mM MgCl2, 25 mM HEPES, pH 7.8, and 1 mM 2-mercaptoethanol with 0.001 ml of reservoir solution containing 20 mM CdCl2, 20 mM MgCl2, 20 mM NiCl2, 24% PEG MME 2000, and 100 mM sodium acetate, pH 4.5, 2 weeks, 19°C, X-ray diffraction structure determination and analysis at 1.46 A resolution, molecular replacement using the structure of the ancestral ADP-dependent kinase (PDB ID 5K27) split into small and large domains as search models Methanosarcina mazei
2.7.1.146 purified enzyme, sitting drop vapor diffusion method, mixing of 0.001 ml of 10 mg/ml protein, 2.5 mM ADP, 200 mM NaCl, 7.5 mM MgCl2, 25 mM HEPES, pH 7.8, and 1 mM 2-mercaptoethanol with 0.001 ml of reservoir solution containing 20 mM CdCl2, 20 mM MgCl2, 20 mM NiCl2, 24% PEG MME 2000, and 100 mM sodium acetate, pH 4.5, 2 weeks, 19°C, X-ray diffraction structure determination and analysis at 2.86 A resolution, molecular replacement using the structure of the ancestral ADP-dependent kinase (PDB ID 5K27) split into small and large domains as search models uncultured bacterium
2.7.1.147 purified enzyme, sitting drop vapor diffusion method, mixing of 0.001 ml of 10 mg/ml protein, 2.5 mM ADP, 200 mM NaCl, 7.5 mM MgCl2, 25 mM HEPES, pH 7.8, and 1 mM 2-mercaptoethanol with 0.001 ml of reservoir solution containing 20 mM CdCl2, 20 mM MgCl2, 20 mM NiCl2, 24% PEG MME 2000, and 100 mM sodium acetate, pH 4.5, 2 weeks, 19°C, X-ray diffraction structure determination and analysis at 1.46 A resolution, molecular replacement using the structure of the ancestral ADP-dependent kinase (PDB ID 5K27) split into small and large domains as search models Methanohalobium evestigatum
2.7.1.147 purified enzyme, sitting drop vapor diffusion method, mixing of 0.001 ml of 10 mg/ml protein, 2.5 mM ADP, 200 mM NaCl, 7.5 mM MgCl2, 25 mM HEPES, pH 7.8, and 1 mM 2-mercaptoethanol with 0.001 ml of reservoir solution containing 20 mM CdCl2, 20 mM MgCl2, 20 mM NiCl2, 24% PEG MME 2000, and 100 mM sodium acetate, pH 4.5, 2 weeks, 19°C, X-ray diffraction structure determination and analysis at 1.46 A resolution, molecular replacement using the structure of the ancestral ADP-dependent kinase (PDB ID 5K27) split into small and large domains as search models Methanosarcina mazei
2.7.1.147 purified enzyme, sitting drop vapor diffusion method, mixing of 0.001 ml of 10 mg/ml protein, 2.5 mM ADP, 200 mM NaCl, 7.5 mM MgCl2, 25 mM HEPES, pH 7.8, and 1 mM 2-mercaptoethanol with 0.001 ml of reservoir solution containing 20 mM CdCl2, 20 mM MgCl2, 20 mM NiCl2, 24% PEG MME 2000, and 100 mM sodium acetate, pH 4.5, 2 weeks, 19°C, X-ray diffraction structure determination and analysis at 2.86 A resolution, molecular replacement using the structure of the ancestral ADP-dependent kinase (PDB ID 5K27) split into small and large domains as search models uncultured bacterium

General Stability

EC Number General Stability Organism
2.7.1.146 enzyme MevePFK/GK protein is unstable at low KCl concentrations and is progressively stabilized at higher concentrations Methanohalobium evestigatum
2.7.1.146 enzyme MmazPFK/GK protein is unstable at low KCl concentrations and is progressively stabilized at higher concentrations Methanosarcina mazei
2.7.1.147 enzyme MevePFK/GK protein is unstable at low KCl concentrations and is progressively stabilized at higher concentrations Methanohalobium evestigatum
2.7.1.147 enzyme MmazPFK/GK protein is unstable at low KCl concentrations and is progressively stabilized at higher concentrations Methanosarcina mazei

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.146 ADP substrate inhibition Methanohalobium evestigatum
2.7.1.146 ADP substrate inhibition Methanosarcina mazei
2.7.1.146 ADP substrate inhibition uncultured bacterium
2.7.1.146 KCl inhibition by KCl is only partially prevented by glycine betaine Methanohalobium evestigatum
2.7.1.146 KCl activates at up to 0.5 M, inhibitory above about 1.0 M, inhibition by KCl is prevented by glycine betaine Methanosarcina mazei
2.7.1.146 NaCl moderate inhibition by 300 mM NaCl or higher concentration Methanohalobium evestigatum
2.7.1.146 NaCl moderate inhibition by 300 mM NaCl or higher concentration Methanosarcina mazei
2.7.1.146 NaCl moderate inhibition by 300 mM NaCl or higher concentration Pyrococcus horikoshii
2.7.1.146 NaCl moderate inhibition by 300 mM NaCl or higher concentration uncultured bacterium
2.7.1.147 ADP substrate inhibition Methanohalobium evestigatum
2.7.1.147 ADP substrate inhibition Methanosarcina mazei
2.7.1.147 ADP substrate inhibition uncultured bacterium
2.7.1.147 KCl inhibition by KCl is only partially prevented by glycine betaine Methanohalobium evestigatum
2.7.1.147 KCl activates at up to 0.5 M, inhibitory above about 1.0 M, inhibition by KCl is prevented by glycine betaine Methanosarcina mazei
2.7.1.147 NaCl moderate inhibition by 300 mM NaCl or higher concentration Methanohalobium evestigatum
2.7.1.147 NaCl moderate inhibition by 300 mM NaCl or higher concentration Methanosarcina mazei
2.7.1.147 NaCl moderate inhibition by 300 mM NaCl or higher concentration uncultured bacterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.146 0.006
-
 D-fructose 6-phosphate pH 6.5, 60°C uncultured bacterium
2.7.1.146 0.0066
-
 D-fructose 6-phosphate pH 6.5, 40°C Methanosarcina mazei
2.7.1.146 0.034
-
 D-fructose 6-phosphate pH 6.5, 40°C Methanohalobium evestigatum
2.7.1.146 0.072
-
 ADP MgADP, pH 6.5, 40°C Methanosarcina mazei
2.7.1.146 0.25
-
 ADP MgADP, pH 6.5, 40°C Methanohalobium evestigatum
2.7.1.146 0.7
-
 ADP MgADP, pH 6.5, 60°C uncultured bacterium
2.7.1.147 0.6
-
 ADP MgADP, pH 7.8, 40°C Methanosarcina mazei
2.7.1.147 1.5
-
 ADP MgADP, pH 7.8, 40°C Methanohalobium evestigatum
2.7.1.147 10.9
-
 D-glucose pH 7.8, 40°C Methanohalobium evestigatum
2.7.1.147 12
-
 D-glucose pH 7.8, 40°C Methanosarcina mazei
2.7.1.147 134
-
 D-glucose pH 7.8, 60°C uncultured bacterium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.146 KCl activates at up to 0.5 M, inhibitory above about 1.0 M Methanosarcina mazei
2.7.1.146 KCl has no evident effect on the AncMsPFK/GK activity uncultured bacterium
2.7.1.146 Mg2+ required uncultured bacterium
2.7.1.146 Mg2+ required Pyrococcus horikoshii
2.7.1.146 Mg2+ required Methanohalobium evestigatum
2.7.1.146 Mg2+ required Methanosarcina mazei
2.7.1.147 KCl activates at up to 0.5 M, inhibitory above about 1.0 M Methanosarcina mazei
2.7.1.147 KCl has no evident effect on the AncMsPFK/GK activity uncultured bacterium
2.7.1.147 Mg2+ required uncultured bacterium
2.7.1.147 Mg2+ required Methanohalobium evestigatum
2.7.1.147 Mg2+ required Methanosarcina mazei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.146 ADP + D-fructose 6-phosphate uncultured bacterium
-
 AMP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.146 ADP + D-fructose 6-phosphate Pyrococcus horikoshii
-
 AMP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.146 ADP + D-fructose 6-phosphate Methanohalobium evestigatum
-
 AMP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.146 ADP + D-fructose 6-phosphate Methanosarcina mazei
-
 AMP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.146 ADP + D-fructose 6-phosphate Methanosarcina mazei OCM 88
-
 AMP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.146 ADP + D-fructose 6-phosphate Methanosarcina mazei DSM 3647
-
 AMP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.146 ADP + D-fructose 6-phosphate Methanosarcina mazei Goe1
-
 AMP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.146 ADP + D-fructose 6-phosphate Methanosarcina mazei JCM 11833
-
 AMP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.146 ADP + D-fructose 6-phosphate Methanohalobium evestigatum DSM 3721
-
 AMP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.146 ADP + D-fructose 6-phosphate Methanohalobium evestigatum OCM 161
-
 AMP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.146 ADP + D-fructose 6-phosphate Pyrococcus horikoshii ATCC 700860
-
 AMP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.146 ADP + D-fructose 6-phosphate Methanohalobium evestigatum Z-7303
-
 AMP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.146 ADP + D-fructose 6-phosphate Methanohalobium evestigatum ATCC BAA-1072
-
 AMP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.146 ADP + D-fructose 6-phosphate Methanohalobium evestigatum NBRC 107634
-
 AMP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.146 ADP + D-fructose 6-phosphate Methanosarcina mazei ATCC BAA-159
-
 AMP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.147 ADP + D-glucose uncultured bacterium
-
 AMP + D-glucose 6-phosphate
-
 ?
2.7.1.147 ADP + D-glucose Methanohalobium evestigatum
-
 AMP + D-glucose 6-phosphate
-
 ?
2.7.1.147 ADP + D-glucose Methanosarcina mazei
-
 AMP + D-glucose 6-phosphate
-
 ?
2.7.1.147 ADP + D-glucose Methanosarcina mazei OCM 88
-
 AMP + D-glucose 6-phosphate
-
 ?
2.7.1.147 ADP + D-glucose Methanosarcina mazei DSM 3647
-
 AMP + D-glucose 6-phosphate
-
 ?
2.7.1.147 ADP + D-glucose Methanosarcina mazei Goe1
-
 AMP + D-glucose 6-phosphate
-
 ?
2.7.1.147 ADP + D-glucose Methanosarcina mazei JCM 11833
-
 AMP + D-glucose 6-phosphate
-
 ?
2.7.1.147 ADP + D-glucose Methanohalobium evestigatum DSM 3721
-
 AMP + D-glucose 6-phosphate
-
 ?
2.7.1.147 ADP + D-glucose Methanohalobium evestigatum OCM 161
-
 AMP + D-glucose 6-phosphate
-
 ?
2.7.1.147 ADP + D-glucose Methanohalobium evestigatum Z-7303
-
 AMP + D-glucose 6-phosphate
-
 ?
2.7.1.147 ADP + D-glucose Methanohalobium evestigatum ATCC BAA-1072
-
 AMP + D-glucose 6-phosphate
-
 ?
2.7.1.147 ADP + D-glucose Methanohalobium evestigatum NBRC 107634
-
 AMP + D-glucose 6-phosphate
-
 ?
2.7.1.147 ADP + D-glucose Methanosarcina mazei ATCC BAA-159
-
 AMP + D-glucose 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.146 Methanohalobium evestigatum D7E8P3
-
-
2.7.1.146 Methanohalobium evestigatum ATCC BAA-1072 D7E8P3
-
-
2.7.1.146 Methanohalobium evestigatum DSM 3721 D7E8P3
-
-
2.7.1.146 Methanohalobium evestigatum NBRC 107634 D7E8P3
-
-
2.7.1.146 Methanohalobium evestigatum OCM 161 D7E8P3
-
-
2.7.1.146 Methanohalobium evestigatum Z-7303 D7E8P3
-
-
2.7.1.146 Methanosarcina mazei Q8PZL9
-
-
2.7.1.146 Methanosarcina mazei ATCC BAA-159 Q8PZL9
-
-
2.7.1.146 Methanosarcina mazei DSM 3647 Q8PZL9
-
-
2.7.1.146 Methanosarcina mazei Goe1 Q8PZL9
-
-
2.7.1.146 Methanosarcina mazei JCM 11833 Q8PZL9
-
-
2.7.1.146 Methanosarcina mazei OCM 88 Q8PZL9
-
-
2.7.1.146 Pyrococcus horikoshii O59355
-
-
2.7.1.146 Pyrococcus horikoshii ATCC 700860 O59355
-
-
2.7.1.146 uncultured bacterium
-
 synthetic construct
-
2.7.1.147 Methanohalobium evestigatum D7E8P3
-
-
2.7.1.147 Methanohalobium evestigatum ATCC BAA-1072 D7E8P3
-
-
2.7.1.147 Methanohalobium evestigatum DSM 3721 D7E8P3
-
-
2.7.1.147 Methanohalobium evestigatum NBRC 107634 D7E8P3
-
-
2.7.1.147 Methanohalobium evestigatum OCM 161 D7E8P3
-
-
2.7.1.147 Methanohalobium evestigatum Z-7303 D7E8P3
-
-
2.7.1.147 Methanosarcina mazei Q8PZL9 cf. EC 2.7.1.146
-
2.7.1.147 Methanosarcina mazei ATCC BAA-159 Q8PZL9 cf. EC 2.7.1.146
-
2.7.1.147 Methanosarcina mazei DSM 3647 Q8PZL9 cf. EC 2.7.1.146
-
2.7.1.147 Methanosarcina mazei Goe1 Q8PZL9 cf. EC 2.7.1.146
-
2.7.1.147 Methanosarcina mazei JCM 11833 Q8PZL9 cf. EC 2.7.1.146
-
2.7.1.147 Methanosarcina mazei OCM 88 Q8PZL9 cf. EC 2.7.1.146
-
2.7.1.147 uncultured bacterium
-
 synthetic construct
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.146 ADP + D-fructose 6-phosphate
-
 uncultured bacterium AMP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.146 ADP + D-fructose 6-phosphate
-
 Pyrococcus horikoshii AMP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.146 ADP + D-fructose 6-phosphate
-
 Methanohalobium evestigatum AMP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.146 ADP + D-fructose 6-phosphate
-
 Methanosarcina mazei AMP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.146 ADP + D-fructose 6-phosphate
-
 Methanosarcina mazei OCM 88 AMP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.146 ADP + D-fructose 6-phosphate
-
 Methanosarcina mazei DSM 3647 AMP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.146 ADP + D-fructose 6-phosphate
-
 Methanosarcina mazei Goe1 AMP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.146 ADP + D-fructose 6-phosphate
-
 Methanosarcina mazei JCM 11833 AMP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.146 ADP + D-fructose 6-phosphate
-
 Methanohalobium evestigatum DSM 3721 AMP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.146 ADP + D-fructose 6-phosphate
-
 Methanohalobium evestigatum OCM 161 AMP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.146 ADP + D-fructose 6-phosphate
-
 Pyrococcus horikoshii ATCC 700860 AMP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.146 ADP + D-fructose 6-phosphate
-
 Methanohalobium evestigatum Z-7303 AMP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.146 ADP + D-fructose 6-phosphate
-
 Methanohalobium evestigatum ATCC BAA-1072 AMP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.146 ADP + D-fructose 6-phosphate
-
 Methanohalobium evestigatum NBRC 107634 AMP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.146 ADP + D-fructose 6-phosphate
-
 Methanosarcina mazei ATCC BAA-159 AMP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.146 additional information the enzyme from Methanohalobium evestigatum also shows glucokinase activity, a bifunctional MevePFK/GK Methanohalobium evestigatum ?
-
-
2.7.1.146 additional information the enzyme from Methanosarcina mazei also shows glucokinase activity, a bifunctional AncMsPFK/GK uncultured bacterium ?
-
-
2.7.1.146 additional information the enzyme from Methanosarcina mazei also shows glucokinase activity, a bifunctional MmazPFK/GK Methanosarcina mazei ?
-
-
2.7.1.146 additional information the enzyme from Methanosarcina mazei also shows glucokinase activity, a bifunctional MmazPFK/GK Methanosarcina mazei OCM 88 ?
-
-
2.7.1.146 additional information the enzyme from Methanosarcina mazei also shows glucokinase activity, a bifunctional MmazPFK/GK Methanosarcina mazei DSM 3647 ?
-
-
2.7.1.146 additional information the enzyme from Methanosarcina mazei also shows glucokinase activity, a bifunctional MmazPFK/GK Methanosarcina mazei Goe1 ?
-
-
2.7.1.146 additional information the enzyme from Methanosarcina mazei also shows glucokinase activity, a bifunctional MmazPFK/GK Methanosarcina mazei JCM 11833 ?
-
-
2.7.1.146 additional information the enzyme from Methanohalobium evestigatum also shows glucokinase activity, a bifunctional MevePFK/GK Methanohalobium evestigatum DSM 3721 ?
-
-
2.7.1.146 additional information the enzyme from Methanohalobium evestigatum also shows glucokinase activity, a bifunctional MevePFK/GK Methanohalobium evestigatum OCM 161 ?
-
-
2.7.1.146 additional information the enzyme from Methanohalobium evestigatum also shows glucokinase activity, a bifunctional MevePFK/GK Methanohalobium evestigatum Z-7303 ?
-
-
2.7.1.146 additional information the enzyme from Methanohalobium evestigatum also shows glucokinase activity, a bifunctional MevePFK/GK Methanohalobium evestigatum ATCC BAA-1072 ?
-
-
2.7.1.146 additional information the enzyme from Methanohalobium evestigatum also shows glucokinase activity, a bifunctional MevePFK/GK Methanohalobium evestigatum NBRC 107634 ?
-
-
2.7.1.146 additional information the enzyme from Methanosarcina mazei also shows glucokinase activity, a bifunctional MmazPFK/GK Methanosarcina mazei ATCC BAA-159 ?
-
-
2.7.1.147 ADP + D-glucose
-
 uncultured bacterium AMP + D-glucose 6-phosphate
-
 ?
2.7.1.147 ADP + D-glucose
-
 Methanohalobium evestigatum AMP + D-glucose 6-phosphate
-
 ?
2.7.1.147 ADP + D-glucose
-
 Methanosarcina mazei AMP + D-glucose 6-phosphate
-
 ?
2.7.1.147 ADP + D-glucose
-
 Methanosarcina mazei OCM 88 AMP + D-glucose 6-phosphate
-
 ?
2.7.1.147 ADP + D-glucose
-
 Methanosarcina mazei DSM 3647 AMP + D-glucose 6-phosphate
-
 ?
2.7.1.147 ADP + D-glucose
-
 Methanosarcina mazei Goe1 AMP + D-glucose 6-phosphate
-
 ?
2.7.1.147 ADP + D-glucose
-
 Methanosarcina mazei JCM 11833 AMP + D-glucose 6-phosphate
-
 ?
2.7.1.147 ADP + D-glucose
-
 Methanohalobium evestigatum DSM 3721 AMP + D-glucose 6-phosphate
-
 ?
2.7.1.147 ADP + D-glucose
-
 Methanohalobium evestigatum OCM 161 AMP + D-glucose 6-phosphate
-
 ?
2.7.1.147 ADP + D-glucose
-
 Methanohalobium evestigatum Z-7303 AMP + D-glucose 6-phosphate
-
 ?
2.7.1.147 ADP + D-glucose
-
 Methanohalobium evestigatum ATCC BAA-1072 AMP + D-glucose 6-phosphate
-
 ?
2.7.1.147 ADP + D-glucose
-
 Methanohalobium evestigatum NBRC 107634 AMP + D-glucose 6-phosphate
-
 ?
2.7.1.147 ADP + D-glucose
-
 Methanosarcina mazei ATCC BAA-159 AMP + D-glucose 6-phosphate
-
 ?
2.7.1.147 additional information the enzyme also shows phosphofructokinase activity, a bifunctional AncMsPFK/GK ancestor enzyme uncultured bacterium ?
-
-
2.7.1.147 additional information the enzyme from Methanohalobium evestigatum also shows phosphofructokinase activity, a bifunctional MevePFK/GK Methanohalobium evestigatum ?
-
-
2.7.1.147 additional information the enzyme from Methanosarcina mazei also shows phosphofructokinase activity, a bifunctional MmazPFK/GK Methanosarcina mazei ?
-
-
2.7.1.147 additional information the enzyme from Methanosarcina mazei also shows phosphofructokinase activity, a bifunctional MmazPFK/GK Methanosarcina mazei OCM 88 ?
-
-
2.7.1.147 additional information the enzyme from Methanosarcina mazei also shows phosphofructokinase activity, a bifunctional MmazPFK/GK Methanosarcina mazei DSM 3647 ?
-
-
2.7.1.147 additional information the enzyme from Methanosarcina mazei also shows phosphofructokinase activity, a bifunctional MmazPFK/GK Methanosarcina mazei Goe1 ?
-
-
2.7.1.147 additional information the enzyme from Methanosarcina mazei also shows phosphofructokinase activity, a bifunctional MmazPFK/GK Methanosarcina mazei JCM 11833 ?
-
-
2.7.1.147 additional information the enzyme from Methanohalobium evestigatum also shows phosphofructokinase activity, a bifunctional MevePFK/GK Methanohalobium evestigatum DSM 3721 ?
-
-
2.7.1.147 additional information the enzyme from Methanohalobium evestigatum also shows phosphofructokinase activity, a bifunctional MevePFK/GK Methanohalobium evestigatum OCM 161 ?
-
-
2.7.1.147 additional information the enzyme from Methanohalobium evestigatum also shows phosphofructokinase activity, a bifunctional MevePFK/GK Methanohalobium evestigatum Z-7303 ?
-
-
2.7.1.147 additional information the enzyme from Methanohalobium evestigatum also shows phosphofructokinase activity, a bifunctional MevePFK/GK Methanohalobium evestigatum ATCC BAA-1072 ?
-
-
2.7.1.147 additional information the enzyme from Methanohalobium evestigatum also shows phosphofructokinase activity, a bifunctional MevePFK/GK Methanohalobium evestigatum NBRC 107634 ?
-
-
2.7.1.147 additional information the enzyme from Methanosarcina mazei also shows phosphofructokinase activity, a bifunctional MmazPFK/GK Methanosarcina mazei ATCC BAA-159 ?
-
-

Synonyms

EC Number Synonyms Comment Organism
2.7.1.146 ADP-dependent phosphofructokinase
-
 uncultured bacterium
2.7.1.146 ADP-dependent phosphofructokinase
-
 Pyrococcus horikoshii
2.7.1.146 ADP-dependent phosphofructokinase
-
 Methanohalobium evestigatum
2.7.1.146 ADP-dependent phosphofructokinase
-
 Methanosarcina mazei
2.7.1.146 ADP-Pfk
-
 uncultured bacterium
2.7.1.146 ADP-Pfk
-
 Pyrococcus horikoshii
2.7.1.146 ADP-Pfk
-
 Methanohalobium evestigatum
2.7.1.146 ADP-Pfk
-
 Methanosarcina mazei
2.7.1.146 AncMsPFK/GK
-
 uncultured bacterium
2.7.1.146 MevePFK/GK
-
 Methanohalobium evestigatum
2.7.1.146 MmazPFK/GK
-
 Methanosarcina mazei
2.7.1.146 More see also EC 2.7.1.147 uncultured bacterium
2.7.1.146 More see also EC 2.7.1.147 Methanohalobium evestigatum
2.7.1.146 More see also EC 2.7.1.147 Methanosarcina mazei
2.7.1.146 pfkC
-
 Methanohalobium evestigatum
2.7.1.147 ADP-dependent glucokinase
-
 uncultured bacterium
2.7.1.147 ADP-dependent glucokinase
-
 Methanohalobium evestigatum
2.7.1.147 ADP-dependent glucokinase
-
 Methanosarcina mazei
2.7.1.147 ADP-GK
-
 uncultured bacterium
2.7.1.147 ADP-GK
-
 Methanohalobium evestigatum
2.7.1.147 ADP-GK
-
 Methanosarcina mazei
2.7.1.147 AncMsPFK/GK
-
 uncultured bacterium
2.7.1.147 MevePFK/GK
-
 Methanohalobium evestigatum
2.7.1.147 MmazPFK/GK
-
 Methanosarcina mazei
2.7.1.147 More see also EC 2.7.1.146 uncultured bacterium
2.7.1.147 More see also EC 2.7.1.146 Methanohalobium evestigatum
2.7.1.147 More see also EC 2.7.1.146 Methanosarcina mazei
2.7.1.147 pfkC
-
 Methanohalobium evestigatum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.146 40
-
 assay at Pyrococcus horikoshii
2.7.1.146 40
-
 assay at Methanohalobium evestigatum
2.7.1.146 40
-
 assay at, phosphofructokinase activity Methanosarcina mazei
2.7.1.146 60
-
 assay at uncultured bacterium
2.7.1.147 40
-
 assay at Methanohalobium evestigatum
2.7.1.147 40
-
 assay at Methanosarcina mazei
2.7.1.147 60
-
 assay at, glucokinase activity uncultured bacterium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.146 4.6
-
 D-fructose 6-phosphate pH 6.5, 40°C Methanosarcina mazei
2.7.1.146 6
-
 D-fructose 6-phosphate pH 6.5, 60°C uncultured bacterium
2.7.1.146 6.1
-
 ADP MgADP, pH 6.5, 40°C Methanosarcina mazei
2.7.1.146 8.7
-
 ADP MgADP, pH 6.5, 60°C uncultured bacterium
2.7.1.146 9.5
-
 D-fructose 6-phosphate pH 6.5, 40°C Methanohalobium evestigatum
2.7.1.146 16
-
 ADP MgADP, pH 6.5, 40°C Methanohalobium evestigatum
2.7.1.147 1.4
-
 D-glucose pH 7.8, 40°C Methanosarcina mazei
2.7.1.147 1.7
-
 ADP MgADP, pH 7.8, 40°C Methanosarcina mazei
2.7.1.147 1.9
-
 D-glucose pH 7.8, 40°C Methanohalobium evestigatum
2.7.1.147 2
-
 ADP MgADP, pH 7.8, 40°C Methanohalobium evestigatum
2.7.1.147 3.6
-
 D-glucose pH 7.8, 60°C uncultured bacterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.146 6.5
-
 assay at uncultured bacterium
2.7.1.146 6.5
-
 assay at Pyrococcus horikoshii
2.7.1.146 6.5
-
 assay at Methanohalobium evestigatum
2.7.1.146 6.5
-
 assay at, phosphofructokinase activity Methanosarcina mazei
2.7.1.147 7.8
-
 assay at Methanohalobium evestigatum
2.7.1.147 7.8
-
 assay at Methanosarcina mazei
2.7.1.147 7.8
-
 assay at, glucokinase activity uncultured bacterium

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.146 ADP dependent on uncultured bacterium
2.7.1.146 ADP dependent on Pyrococcus horikoshii
2.7.1.146 ADP dependent on Methanohalobium evestigatum
2.7.1.146 ADP dependent on Methanosarcina mazei
2.7.1.147 ADP dependent on uncultured bacterium
2.7.1.147 ADP dependent on Methanohalobium evestigatum
2.7.1.147 ADP dependent on Methanosarcina mazei

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.146 0.003
-
 ADP MgADP, pH 6.5, 40°C Methanohalobium evestigatum
2.7.1.146 0.006
-
 ADP MgADP, pH 6.5, 60°C uncultured bacterium
2.7.1.146 0.0061
-
 ADP MgADP, pH 6.5, 40°C Methanosarcina mazei
2.7.1.147 0.003
-
 ADP MgADP, pH 7.8, 40°C Methanohalobium evestigatum
2.7.1.147 0.006
-
 ADP MgADP, pH 7.8, 60°C uncultured bacterium
2.7.1.147 0.0061
-
 ADP MgADP, pH 7.8, 40°C Methanosarcina mazei

General Information

EC Number General Information Comment Organism
2.7.1.146 evolution the enzyme belongs to the ADP-dependent phosphofructokinase/glucokinase family. Homology modeling of ADP-dependent sugar kinases from Halobacteria, Methanosarcinales and Eukarya, overview. Models built are divided into four groups based on the taxonomic categorization of the source organism and their ability to grow in high salinity environments as reported in the literature. The groups defined are: Halobacteria, halophilic Methanosarcinales, non-halophilic Methanosarcinales, and Eukarya, the latter used as a control outgroup. Analsis of enzyme ADP-PFK structure (PDB ID 1U2X) from Pyrococcus furiosus. Sequences and structures comparisons. Pyrococcus horikoshii posseses a phosphofructokinase and a glucokinase Pyrococcus horikoshii
2.7.1.146 evolution the enzyme belongs to the ADP-dependent phosphofructokinase/glucokinase family. Homology modeling of ADP-dependent sugar kinases from Halobacteria, Methanosarcinales and Eukarya, overview. Models built are divided into four groups based on the taxonomic categorization of the source organism and their ability to grow in high salinity environments as reported in the literature. The groups defined are: Halobacteria, halophilic Methanosarcinales, non-halophilic Methanosarcinales, and Eukarya, the latter used as a control outgroup. Sequences and structures comparisons uncultured bacterium
2.7.1.146 evolution the enzyme belongs to the ADP-dependent phosphofructokinase/glucokinase family. Homology modeling of ADP-dependent sugar kinases from Halobacteria, Methanosarcinales and Eukarya, overview. Models built are divided into four groups based on the taxonomic categorization of the source organism and their ability to grow in high salinity environments as reported in the literature. The groups defined are: Halobacteria, halophilic Methanosarcinales, non-halophilic Methanosarcinales, and Eukarya, the latter used as a control outgroup. Sequences and structures comparisons. Methanohalobium evestigatum posseses a bifunctional MevePFK/GK Methanohalobium evestigatum
2.7.1.146 evolution the enzyme belongs to the ADP-dependent phosphofructokinase/glucokinase family. Homology modeling of ADP-dependent sugar kinases from Halobacteria, Methanosarcinales and Eukarya, overview. Models built are divided into four groups based on the taxonomic categorization of the source organism and their ability to grow in high salinity environments as reported in the literature. The groups defined are: Halobacteria, halophilic Methanosarcinales, non-halophilic Methanosarcinales, and Eukarya, the latter used as a control outgroup. Sequences and structures comparisons. Methanosarcina mazei possesses a bifunctional MmazPFK/GK Methanosarcina mazei
2.7.1.147 evolution the enzyme belongs to the ADP-dependent phosphofructokinase/glucokinase family. Homology modeling of ADP-dependent sugar kinases from Halobacteria, Methanosarcinales and Eukarya, overview. Models built are divided into four groups based on the taxonomic categorization of the source organism and their ability to grow in high salinity environments as reported in the literature. The groups defined are: Halobacteria, halophilic Methanosarcinales, non-halophilic Methanosarcinales, and Eukarya, the latter used as a control outgroup. Sequences and structures comparisons uncultured bacterium
2.7.1.147 evolution the enzyme belongs to the ADP-dependent phosphofructokinase/glucokinase family. Homology modeling of ADP-dependent sugar kinases from Halobacteria, Methanosarcinales and Eukarya, overview. Models built are divided into four groups based on the taxonomic categorization of the source organism and their ability to grow in high salinity environments as reported in the literature. The groups defined are: Halobacteria, halophilic Methanosarcinales, non-halophilic Methanosarcinales, and Eukarya, the latter used as a control outgroup. Sequences and structures comparisons. Methanohalobium evestigatum posseses a bifunctional MevePFK/GK Methanohalobium evestigatum
2.7.1.147 evolution the enzyme belongs to the ADP-dependent phosphofructokinase/glucokinase family. Homology modeling of ADP-dependent sugar kinases from Halobacteria, Methanosarcinales and Eukarya, overview. Models built are divided into four groups based on the taxonomic categorization of the source organism and their ability to grow in high salinity environments as reported in the literature. The groups defined are: Halobacteria, halophilic Methanosarcinales, non-halophilic Methanosarcinales, and Eukarya, the latter used as a control outgroup. Sequences and structures comparisons. Methanosarcina mazei posseses a bifunctional MmazPFK/GK Methanosarcina mazei

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.146 12.43
-
 ADP MgADP, pH 6.5, 60°C uncultured bacterium
2.7.1.146 64
-
 ADP MgADP, pH 6.5, 40°C Methanohalobium evestigatum
2.7.1.146 84.7
-
 ADP MgADP, pH 6.5, 40°C Methanosarcina mazei
2.7.1.146 279.4
-
 D-fructose 6-phosphate pH 6.5, 40°C Methanohalobium evestigatum
2.7.1.146 697
-
 D-fructose 6-phosphate pH 6.5, 40°C Methanosarcina mazei
2.7.1.146 1000
-
 D-fructose 6-phosphate pH 6.5, 60°C uncultured bacterium
2.7.1.147 0.027
-
 D-glucose pH 7.8, 60°C uncultured bacterium
2.7.1.147 0.117
-
 D-glucose pH 7.8, 40°C Methanosarcina mazei
2.7.1.147 0.17
-
 D-glucose pH 7.8, 40°C Methanohalobium evestigatum
2.7.1.147 1.33
-
 ADP MgADP, pH 7.8, 40°C Methanohalobium evestigatum
2.7.1.147 2.83
-
 ADP MgADP, pH 7.8, 40°C Methanosarcina mazei