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Literature summary extracted from

  • Lopez, M.G.; Irla, M.; Brito, L.F.; Wendisch, V.F.
    Characterization of D-arabitol as newly discovered carbon source of Bacillus methanolicus (2019), Front. Microbiol., 10, 1725 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.17 gene mtlD, transcriptional organization of the gene cluster, quantitative RT-PCR enzyme expression analysis Bacillus methanolicus
1.1.1.301 gene atlD, cloning of genes coding for arabitol utilization pathway, arabitol utilization genes are co-transcribed in an operon, a gene cluster comprising eight genes that is upregulated during growth with arabitol as a sole carbon source, transcriptional organization of the gene cluster, transcriptome sequencing, and real-time quantitative reverse transcription PCR enzyme expression analysis. Recombinant expression of atlD in Corynebacterium glutamicum strain ATCC 13032 mtlD deletion mutant complements the mutant and restores growth of the Corynebacterium glutamicum DELTAmtlD mutant with arabitol Bacillus methanolicus

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.17 additional information functional complementation of arabitol dehydrogenase activity of gene MtlD in Corynebacterium glutamicum DELTAmtlD deletion mutant by gene atlD of Bacillus methanolicus strain MG3 Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.17 D-arabitol 1-phosphate + NAD+ Bacillus methanolicus
-
 D-xylulose 5-phosphate + NADH + H+
-
 r
1.1.1.17 D-arabitol 1-phosphate + NAD+ Bacillus methanolicus ATCC 53907
-
 D-xylulose 5-phosphate + NADH + H+
-
 r
1.1.1.17 D-arabitol 1-phosphate + NAD+ Bacillus methanolicus MGA3
-
 D-xylulose 5-phosphate + NADH + H+
-
 r
1.1.1.17 D-mannitol 1-phosphate + NAD+ Bacillus methanolicus
-
 D-fructose 6-phosphate + NADH + H+
-
 r
1.1.1.17 D-mannitol 1-phosphate + NAD+ Corynebacterium glutamicum
-
 D-fructose 6-phosphate + NADH + H+
-
 r
1.1.1.17 D-mannitol 1-phosphate + NAD+ Bacillus methanolicus ATCC 53907
-
 D-fructose 6-phosphate + NADH + H+
-
 r
1.1.1.17 D-mannitol 1-phosphate + NAD+ Bacillus methanolicus MGA3
-
 D-fructose 6-phosphate + NADH + H+
-
 r
1.1.1.301 D-arabitol 1-phosphate + NAD+ Bacillus methanolicus
-
 D-xylulose 5-phosphate + NADH + H+
-
 ?
1.1.1.301 D-arabitol 1-phosphate + NAD+ Bacillus methanolicus MGA3
-
 D-xylulose 5-phosphate + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.17 Bacillus methanolicus I3E3X0
-
-
1.1.1.17 Bacillus methanolicus ATCC 53907 I3E3X0
-
-
1.1.1.17 Bacillus methanolicus MGA3 I3E3X0
-
-
1.1.1.17 Corynebacterium glutamicum A0A0U4Y4V3
-
-
1.1.1.301 Bacillus methanolicus
-
-
-
1.1.1.301 Bacillus methanolicus MGA3
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.17 additional information growth of Bacillus methanolicus strain MGA3 on arabitol as single carbon source and as co-substrate to mannitol. Although arabitol supports growth of strain MGA3 as a sole source of carbon and energy, the strain grows at a lower growth rate than with its preferred sugar alcohol substrate mannitol. Mannitol is utilized faster than arabitol and co-consumption of both sugar alcohols is observed, overview Bacillus methanolicus
-
1.1.1.301 culture condition:D-arabitol-grown cell D-arabitol is a carbon source of Bacillus methanolicus Bacillus methanolicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.17 0.02
-
 arabitol phosphate dehydrogenase activity in mannitol grown cells of strains MG3, pH 7.2, 30°C Bacillus methanolicus
1.1.1.17 0.05
-
 arabitol phosphate dehydrogenase activity in arabitol grown cells of strains MG3, pH 7.2, 30°C Bacillus methanolicus
1.1.1.301 0.05
-
 crude extracts of Bacillus methanolicus cells grown on arabitol, pH 7.2, 30°C Bacillus methanolicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.17 D-arabitol 1-phosphate + NAD+
-
 Bacillus methanolicus D-xylulose 5-phosphate + NADH + H+
-
 r
1.1.1.17 D-arabitol 1-phosphate + NAD+
-
 Corynebacterium glutamicum D-xylulose 5-phosphate + NADH + H+
-
 r
1.1.1.17 D-arabitol 1-phosphate + NAD+
-
 Bacillus methanolicus ATCC 53907 D-xylulose 5-phosphate + NADH + H+
-
 r
1.1.1.17 D-arabitol 1-phosphate + NAD+
-
 Bacillus methanolicus MGA3 D-xylulose 5-phosphate + NADH + H+
-
 r
1.1.1.17 D-mannitol 1-phosphate + NAD+
-
 Bacillus methanolicus D-fructose 6-phosphate + NADH + H+
-
 r
1.1.1.17 D-mannitol 1-phosphate + NAD+
-
 Corynebacterium glutamicum D-fructose 6-phosphate + NADH + H+
-
 r
1.1.1.17 D-mannitol 1-phosphate + NAD+
-
 Bacillus methanolicus ATCC 53907 D-fructose 6-phosphate + NADH + H+
-
 r
1.1.1.17 D-mannitol 1-phosphate + NAD+
-
 Bacillus methanolicus MGA3 D-fructose 6-phosphate + NADH + H+
-
 r
1.1.1.301 D-arabitol 1-phosphate + NAD+
-
 Bacillus methanolicus D-xylulose 5-phosphate + NADH + H+
-
 ?
1.1.1.301 D-arabitol 1-phosphate + NAD+
-
 Bacillus methanolicus MGA3 D-xylulose 5-phosphate + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.17 BMMGA3_RS01080 locus name Bacillus methanolicus
1.1.1.17 More cf. EC 1.1.1.301 Bacillus methanolicus
1.1.1.17 MtlD
-
 Bacillus methanolicus
1.1.1.17 MtlD
-
 Corynebacterium glutamicum
1.1.1.301 arabitol phosphate dehydrogenase
-
 Bacillus methanolicus
1.1.1.301 atlD
-
 Bacillus methanolicus
1.1.1.301 D-arabitol phosphate dehydrogenase
-
 Bacillus methanolicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.17 30
-
 assay at Bacillus methanolicus
1.1.1.301 30
-
 assay at Bacillus methanolicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.17 7.2
-
 assay at Bacillus methanolicus
1.1.1.301 7.2
-
 assay at Bacillus methanolicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.17 NAD+
-
 Bacillus methanolicus
1.1.1.17 NAD+
-
 Corynebacterium glutamicum
1.1.1.17 NADH
-
 Bacillus methanolicus
1.1.1.17 NADH
-
 Corynebacterium glutamicum

Expression

EC Number Organism Comment Expression
1.1.1.17 Bacillus methanolicus in Bacillus methanolicus, RNA levels for atlD are higher while lower for mtlD during growth with both carbon sources as compared to growth with mannitol alone down
1.1.1.17 Corynebacterium glutamicum in Corynebacterium glutamicum, arabitol induces expression of genes rbtT, mtlD, sixA, xylB and atlR, and glucose represses expression of mtlD down
1.1.1.17 Bacillus methanolicus differential gene expression analysis of Bacillus methanolicus strain MGA3 cells grown on arabitol as compared to mannitol via transcriptome sequencing (RNA-seq), quantitative RT-PCR expression analysis, the enzyme can be induced by arabitol additional information
1.1.1.301 Bacillus methanolicus the enzyme is induced by D-arabitol up

General Information

EC Number General Information Comment Organism
1.1.1.17 malfunction the Corynebacterium glutamicum arabitol-negative DELTAmtlD deletion mutant can be complemented by heterologous expression of Bacillus methanolicus strain MGA3 operon atlABCD or gene atlD Corynebacterium glutamicum
1.1.1.17 metabolism the enzyme is involved in the arabitol catabolism and the pentose phosphate pathway, model of the pathways for arabitol catabolism in bacteria, overview Bacillus methanolicus
1.1.1.17 physiological function Corynebacterium glutamicum is a natural D-arabitol utilizer that requires arabitol-utilizing mannitol-1-phosphate 5-dehydrogenase MtlD for arabitol catabolism Corynebacterium glutamicum
1.1.1.301 metabolism identification of genes coding for arabitol utilization pathway, overview. Bacillus methanolicus possesses an arabitol inducible operon encoding, amongst others, a putative PTS system and an arabitol phosphate dehydrogenase for uptake and activation of arabitol as growth substrate. Model of the pathways for arabitol catabolism in bacteria, overview Bacillus methanolicus