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Literature summary extracted from

  • Zhou, Y.; Peng, Q.; Zhang, L.; Cheng, S.; Zeng, L.; Dong, F.; Yang, Z.
    Characterization of enzymes specifically producing chiral flavor compounds (R)- and (S)-1-phenylethanol from tea (Camellia sinensis) flowers (2019), Food Chem., 280, 27-33 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.B3 expressed in Escherichia coli Rosetta (DE3) cells and Arabidopsis thaliana protoplast Camellia sinensis
1.1.1.B4 expressed in Escherichia coli Rosetta (DE3) cells and Arabidopsis thaliana protoplast Camellia sinensis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.B3 cytoplasm
-
 Camellia sinensis 5737
-
1.1.1.B4 cytoplasm
-
 Camellia sinensis 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.B3 additional information the addition of Mg2+ does not affect the enzyme activity significantly Camellia sinensis
1.1.1.B4 additional information the addition of Mg2+ does not affect the enzyme activity significantly Camellia sinensis

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.B3 Camellia sinensis A0A494WD27 cultivar Jinxuan
-
1.1.1.B4 Camellia sinensis A0A494WKI6 cultivar Jinxuan
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.B3 Ni Sepharose column chromatography Camellia sinensis
1.1.1.B4 Ni Sepharose column chromatography Camellia sinensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.B3 flower
-
 Camellia sinensis
-
1.1.1.B3 leaf
-
 Camellia sinensis
-
1.1.1.B4 flower
-
 Camellia sinensis
-
1.1.1.B4 leaf
-
 Camellia sinensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.B3 acetophenone + NADPH + H+
-
 Camellia sinensis (1S)-1-phenylethanol + NADP+
-
 ?
1.1.1.B4 acetophenone + NADPH + H+
-
 Camellia sinensis (1R)-1-phenylethanol + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.B3 ? x * 29040, calculated from amino acid sequence Camellia sinensis
1.1.1.B4 ? x * 28660, calculated from amino acid sequence Camellia sinensis

Synonyms

EC Number Synonyms Comment Organism
1.1.1.B3 (S)-1-phenylethanolsynthase
-
 Camellia sinensis
1.1.1.B3 SDR
-
 Camellia sinensis
1.1.1.B3 short chain dehydrogenase
-
 Camellia sinensis
1.1.1.B3 SPES
-
 Camellia sinensis
1.1.1.B4 (R)-1-phenylethanolsynthase
-
 Camellia sinensis
1.1.1.B4 RPES
-
 Camellia sinensis
1.1.1.B4 SDR
-
 Camellia sinensis
1.1.1.B4 short chain dehydrogenase
-
 Camellia sinensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.B3 45
-
-
 Camellia sinensis
1.1.1.B4 55
-
-
 Camellia sinensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.B3 8
-
-
 Camellia sinensis
1.1.1.B4 8
-
-
 Camellia sinensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.B3 NADPH strong preference for NADPH as cofactor Camellia sinensis
1.1.1.B4 NADPH strong preference for NADPH as cofactor Camellia sinensis