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Literature summary extracted from

  • Khine, A.; Yang, M.; Hu, A.; Lin, G.; Toh, Y.; Chen, H.
    Production of optically pure (-)-borneol by Pseudomonas monteilii TCU-CK1 and characterization of borneol dehydrogenase involved (2020), Enzyme Microb. Technol., 139, 109586 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.198 expressed in Escherichia coli BL21(DE3) cells Pseudomonas monteilii

General Stability

EC Number General Stability Organism
1.1.1.198 The enzyme activity is significantly inhibited by phosphate buffer in the alkaline range Pseudomonas monteilii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.198 (+)-Camphor
-
 Pseudomonas monteilii
1.1.1.198 (-)-Camphor
-
 Pseudomonas monteilii
1.1.1.227 (-)-Camphor
-
 Pseudomonas monteilii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.198 0.13
-
 (+)-borneol at pH 11.0 and 25°C Pseudomonas monteilii
1.1.1.198 0.13
-
 (+)-borneol pH 11, 25°C Pseudomonas monteilii
1.1.1.198 0.14
-
 (+)-borneol pH 7, 25°C Pseudomonas monteilii
1.1.1.198 0.14
-
 (+)-borneol at pH 7.0 and 25°C Pseudomonas monteilii
1.1.1.198 0.64
-
 (-)-borneol at pH 7.0 and 25°C Pseudomonas monteilii
1.1.1.198 0.85
-
 (-)-borneol at pH 11.0 and 25°C Pseudomonas monteilii
1.1.1.227 0.64
-
 (-)-borneol pH 7, 25°C Pseudomonas monteilii
1.1.1.227 0.85
-
 (-)-borneol pH 11, 25°C Pseudomonas monteilii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.227 (-)-borneol + NAD+ Pseudomonas monteilii
-
 (-)-camphor + NADH + H+
-
 ?
1.1.1.227 additional information Pseudomonas monteilii (+)-borneol is the preferred substrate, reaction of EC 1.1.1.198 ?
-
-

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.198 Pseudomonas monteilii
-
-
-
1.1.1.198 Pseudomonas monteilii TCU-CK1
-
-
-
1.1.1.227 Pseudomonas monteilii
-
-
-
1.1.1.227 Pseudomonas monteilii TCU-CK1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.198 partial, using Phenyl-Sepharose hydrophobic interaction, Q-Sepharose Fast Flow and Mono-Q anion-exchange columns Pseudomonas monteilii
1.1.1.198 phenyl-Sepharose column chromatography, Q-Sepharose column chromatography and MonoQ column chromatography Pseudomonas monteilii
1.1.1.227 partial, using Phenyl-Sepharose hydrophobic interaction, Q-Sepharose Fast Flow and Mono-Q anion-exchange columns Pseudomonas monteilii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.198 (+)-borneol + NAD+
-
 Pseudomonas monteilii (+)-camphor + NADH + H+
-
 ?
1.1.1.198 (+)-borneol + NAD+ (+)-borneol is the preferred substrate Pseudomonas monteilii (+)-camphor + NADH + H+
-
 ?
1.1.1.198 (+)-borneol + NAD+
-
 Pseudomonas monteilii TCU-CK1 (+)-camphor + NADH + H+
-
 ?
1.1.1.198 (+)-borneol + NAD+ (+)-borneol is the preferred substrate Pseudomonas monteilii TCU-CK1 (+)-camphor + NADH + H+
-
 ?
1.1.1.198 (-)-borneol + NAD+
-
 Pseudomonas monteilii (-)-camphor + NADH + H+
-
 ?
1.1.1.198 (-)-borneol + NAD+
-
 Pseudomonas monteilii TCU-CK1 (-)-camphor + NADH + H+
-
 ?
1.1.1.198 additional information (-)-borneol is an additional substrate, reaction of EC 1.1.1.227. (+)-Borneol is the preferred substrate Pseudomonas monteilii ?
-
-
1.1.1.198 additional information the recombinant enzyme does not use isopropanol, cyclohexanol, cyclopentanol, or(+/-)-2-butanol as alternative substrates, and NADP+ as a cofactor Pseudomonas monteilii ?
-
-
1.1.1.198 additional information (-)-borneol is an additional substrate, reaction of EC 1.1.1.227. (+)-Borneol is the preferred substrate Pseudomonas monteilii TCU-CK1 ?
-
-
1.1.1.198 additional information the recombinant enzyme does not use isopropanol, cyclohexanol, cyclopentanol, or(+/-)-2-butanol as alternative substrates, and NADP+ as a cofactor Pseudomonas monteilii TCU-CK1 ?
-
-
1.1.1.227 (-)-borneol + NAD+
-
 Pseudomonas monteilii (-)-camphor + NADH + H+
-
 ?
1.1.1.227 additional information (+)-borneol is the preferred substrate, reaction of EC 1.1.1.198 Pseudomonas monteilii ?
-
-

Subunits

EC Number Subunits Comment Organism
1.1.1.198 ? x * 30000, SDS-PAGE Pseudomonas monteilii
1.1.1.198 ? x * 27400, calculated from amino acid sequence Pseudomonas monteilii
1.1.1.198 ? x * 30000, SDS-PAGE, x * 27400, calculated from sequence Pseudomonas monteilii
1.1.1.227 ? x * 30000, SDS-PAGE, x * 27400, calculated from sequence Pseudomonas monteilii

Synonyms

EC Number Synonyms Comment Organism
1.1.1.198 BDH
-
 Pseudomonas monteilii
1.1.1.198 BDH cf. EC 1.1.1.227 Pseudomonas monteilii
1.1.1.198 TCK1_3449
-
 Pseudomonas monteilii
1.1.1.227 BDH cf. EC1.1.1.198 Pseudomonas monteilii
1.1.1.227 TCK1_3449
-
 Pseudomonas monteilii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.198 11.3
-
 (-)-borneol at pH 7.0 and 25°C Pseudomonas monteilii
1.1.1.198 29.4
-
 (-)-borneol at pH 11.0 and 25°C Pseudomonas monteilii
1.1.1.198 75
-
 (+)-borneol pH 7, 25°C Pseudomonas monteilii
1.1.1.198 75
-
 (+)-borneol at pH 7.0 and 25°C Pseudomonas monteilii
1.1.1.198 209
-
 (+)-borneol at pH 11.0 and 25°C Pseudomonas monteilii
1.1.1.198 209
-
 (+)-borneol pH 11, 25°C Pseudomonas monteilii
1.1.1.227 11.3
-
 (-)-borneol pH 7, 25°C Pseudomonas monteilii
1.1.1.227 29.4
-
 (-)-borneol pH 11, 25°C Pseudomonas monteilii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.198 11
-
-
 Pseudomonas monteilii
1.1.1.227 12.5
-
-
 Pseudomonas monteilii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.198 NAD+
-
 Pseudomonas monteilii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.198 0.29
-
 (+)-Camphor pH 7, 25°C Pseudomonas monteilii
1.1.1.198 0.29
-
 (+)-Camphor at pH 7.0 and 25°C Pseudomonas monteilii
1.1.1.198 0.98
-
 (+)-Camphor at pH 11.0 and 25°C Pseudomonas monteilii
1.1.1.198 0.98
-
 (+)-Camphor pH 11, 25°C Pseudomonas monteilii
1.1.1.198 1.03
-
 (-)-Camphor at pH 7.0 and 25°C Pseudomonas monteilii
1.1.1.198 1.07
-
 (-)-Camphor at pH 11.0 and 25°C Pseudomonas monteilii
1.1.1.227 1.03
-
 (-)-Camphor pH 7, 25°C Pseudomonas monteilii
1.1.1.227 1.07
-
 (-)-Camphor pH 11, 25°C Pseudomonas monteilii

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.198 Pseudomonas monteilii calculated from amino acid sequence
-
 4.8
1.1.1.198 Pseudomonas monteilii calculated fromn sequence
-
 4.8
1.1.1.227 Pseudomonas monteilii calculated fromn sequence
-
 4.8

General Information

EC Number General Information Comment Organism
1.1.1.198 physiological function neither wild-tpe nor a gene deletion mutant is able to grow on (-)-borneol or (-)-camphor as sole carbon source. Both strains grow similarly on (+)-borneol or (+)-camphor Pseudomonas monteilii
1.1.1.227 physiological function neither wild-tpe nor a gene deletion mutant is able to grow on (-)-borneol or (-)-camphor as sole carbon source. Both strains grow similarly on (+)-borneol or (+)-camphor Pseudomonas monteilii