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Literature summary extracted from

  • Parker, V.S.; Squirewell, E.J.; Lehmler, H.J.; Robertson, L.W.; Duffel, M.W.
    Hydroxylated and sulfated metabolites of commonly occurring airborne polychlorinated biphenyls inhibit human steroid sulfotransferases SULT1E1 and SULT2A1 (2018), Environ. Toxicol. Pharmacol., 58, 196-201 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.2.2 recombinant expression of the enzyme in Escherichia coli strain BL21(DE3) Homo sapiens
2.8.2.4 recombinant expression of the enzyme in Escherichia coli strain BL21(DE3) Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.2.2 2,2',5,5'-tetrachloro-4-biphenylsulfate
-
 Homo sapiens
2.8.2.2 2,2',5,5'-tetrachloro-4-hydroxybiphenyl
-
 Homo sapiens
2.8.2.2 2,3',4-trichloro-4'-biphenylsulfate
-
 Homo sapiens
2.8.2.2 2,3',4-trichloro-4'-hydroxybiphenyl
-
 Homo sapiens
2.8.2.2 2,4'-dichloro-4-biphenylsulfate
-
 Homo sapiens
2.8.2.2 2,4'-dichloro-4-hydroxybiphenyl
-
 Homo sapiens
2.8.2.2 3,3'-dichloro-4-biphenylsulfate
-
 Homo sapiens
2.8.2.2 3,3'-dichloro-4-hydroxybiphenyl
-
 Homo sapiens
2.8.2.2 4-chloro-4'-biphenylsulfate
-
 Homo sapiens
2.8.2.2 4-chloro-4'-hydroxybiphenyl
-
 Homo sapiens
2.8.2.2 additional information hydroxylated and sulfated metabolites of commonly occurring airborne polychlorinated biphenyls inhibit human steroid sulfotransferases SULT1E1 (EC 2.8.2.4) and SULT2A1. No inhibition by 3,3'-dichloro-4-biphenylsulfate at up to 0.01 mM Homo sapiens
2.8.2.4 2,2',5,5'-tetrachloro-4-hydroxybiphenyl
-
 Homo sapiens
2.8.2.4 2,3',4-trichloro-4'-hydroxybiphenyl the inhibitor has been identified as a metabolite in human plasma with an estimated half-life of 6.5 years Homo sapiens
2.8.2.4 2,4'-dichloro-4-hydroxybiphenyl
-
 Homo sapiens
2.8.2.4 3,3'-dichloro-4-hydroxybiphenyl
-
 Homo sapiens
2.8.2.4 4-chloro-4'-hydroxybiphenyl
-
 Homo sapiens
2.8.2.4 additional information hydroxylated and sulfated metabolites of commonly occurring airborne polychlorinated biphenyls inhibit human steroid sulfotransferases SULT1E1 and SULT2A1 (EC 2.8.2.2). No inhibition by 4-chloro-4'-hydroxybiphenylsulfate, 2,4'-dichloro-4-hydroxybiphenylsulfate, 3,3'-dichloro-4-hydroxybiphenylsulfate, and 2,2',5,5'-tetrachloro-4-hydroxybiphenylsulfate at up to 0.01 mM Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.2.2 3'-phosphoadenylyl sulfate + dehydroepiandrosterone Homo sapiens
-
 adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
-
 ?
2.8.2.2 additional information Homo sapiens SULT2A1 catalyzes the sulfation of hydroxysteroids such as dehydroepiandrosterone and various alcohol-containing androgens ?
-
-
2.8.2.4 3'-phosphoadenylyl sulfate + 17beta-estradiol Homo sapiens
-
 adenosine 3',5'-bisphosphate + 17beta-estradiol sulfate
-
 ?
2.8.2.4 additional information Homo sapiens SULT1E1 catalyzes the sulfation of estradiol and other phenolic steroids ?
-
-

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.2 Homo sapiens Q06520
-
-
2.8.2.4 Homo sapiens P49888
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.2 recombinant enzyme from Escherichia coli strain BL21(DE3) Homo sapiens
2.8.2.4 recombinant enzyme from Escherichia coli strain BL21(DE3) Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.2 3'-phosphoadenylyl sulfate + dehydroepiandrosterone
-
 Homo sapiens adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate
-
 ?
2.8.2.2 additional information SULT2A1 catalyzes the sulfation of hydroxysteroids such as dehydroepiandrosterone and various alcohol-containing androgens Homo sapiens ?
-
-
2.8.2.4 3'-phosphoadenylyl sulfate + 17beta-estradiol
-
 Homo sapiens adenosine 3',5'-bisphosphate + 17beta-estradiol sulfate
-
 ?
2.8.2.4 3'-phosphoadenylyl sulfate + 17beta-estradiol SULT1E1 has a high affinity and catalytic efficiency for estradiol as substrate Homo sapiens adenosine 3',5'-bisphosphate + 17beta-estradiol sulfate
-
 ?
2.8.2.4 additional information SULT1E1 catalyzes the sulfation of estradiol and other phenolic steroids Homo sapiens ?
-
-

Synonyms

EC Number Synonyms Comment Organism
2.8.2.2 alcohol/hydroxysteroid sulfotransferase
-
 Homo sapiens
2.8.2.2 steroid sulfotransferase
-
 Homo sapiens
2.8.2.2 SULT2A1
-
 Homo sapiens
2.8.2.4 estrogen sulfotransferase
-
 Homo sapiens
2.8.2.4 steroid sulfotransferase
-
 Homo sapiens
2.8.2.4 SULT1E1
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.2.2 37
-
 assay at Homo sapiens
2.8.2.4 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.2 7.4
-
 assay at Homo sapiens
2.8.2.4 7.4
-
 assay at Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.8.2.2 additional information
-
 additional information inhibition kinetics Homo sapiens
2.8.2.4 additional information
-
 additional information inhibition kinetics Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.8.2.2 0.0013
-
 recombinant SULT2A1, pH 7.4, 37°C Homo sapiens 2,2',5,5'-tetrachloro-4-hydroxybiphenyl
2.8.2.2 0.0015
-
 recombinant SULT2A1, pH 7.4, 37°C Homo sapiens 2,4'-dichloro-4-hydroxybiphenyl
2.8.2.2 0.0049
-
 recombinant SULT2A1, pH 7.4, 37°C Homo sapiens 2,3',4-trichloro-4'-hydroxybiphenyl
2.8.2.2 0.012
-
 recombinant SULT2A1, pH 7.4, 37°C Homo sapiens 4-chloro-4'-hydroxybiphenyl
2.8.2.2 0.023
-
 recombinant SULT2A1, pH 7.4, 37°C Homo sapiens 3,3'-dichloro-4-hydroxybiphenyl
2.8.2.2 0.03
-
 recombinant SULT2A1, pH 7.4, 37°C Homo sapiens 2,2',5,5'-tetrachloro-4-biphenylsulfate
2.8.2.2 0.046
-
 recombinant SULT2A1, pH 7.4, 37°C Homo sapiens 2,4'-dichloro-4-biphenylsulfate
2.8.2.4 0.0000072
-
 recombinant SULT1E1, pH 7.4, 37°C Homo sapiens 3,3'-dichloro-4-hydroxybiphenyl
2.8.2.4 0.0000073
-
 recombinant SULT1E1, pH 7.4, 37°C Homo sapiens 2,3',4-trichloro-4'-hydroxybiphenyl
2.8.2.4 0.000018
-
 recombinant SULT1E1, pH 7.4, 37°C Homo sapiens 2,4'-dichloro-4-hydroxybiphenyl
2.8.2.4 0.000019
-
 recombinant SULT1E1, pH 7.4, 37°C Homo sapiens 2,2',5,5'-tetrachloro-4-hydroxybiphenyl
2.8.2.4 0.00044
-
 recombinant SULT1E1, pH 7.4, 37°C Homo sapiens 2,3',4-trichloro-4'-biphenylsulfate
2.8.2.4 0.0013
-
 recombinant SULT1E1, pH 7.4, 37°C Homo sapiens 4-chloro-4'-hydroxybiphenyl

General Information

EC Number General Information Comment Organism
2.8.2.2 metabolism both SULT1E1 and SULT2A1 can play important roles in steroid signaling through regulation of the active forms of the hormones Homo sapiens
2.8.2.2 physiological function SULT2A1 catalyzes the formation of dehydroepiandrosterone sulfate (DHEAS) the most abundant circulating steroid in humans. DHEAS serves as a transport form for dehydroepiandrosterone (DHEA) in the serum. Following uptake of DHEAS by tissues and its subsequent hydrolysis catalyzed by steroid sulfatase, DHEA serves as a precursor for both androgens and estrogens Homo sapiens
2.8.2.4 metabolism both SULT1E1 and SULT2A1 can play important roles in steroid signaling through regulation of the active forms of the hormones Homo sapiens
2.8.2.4 physiological function SULT1E1 is involved in estrogenic signaling through regulation of intracellular concentrations of the active hormone Homo sapiens