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Literature summary extracted from

  • Frey, J.; Schneider, F.; Huhn, T.; Spiteller, D.; Schink, B.; Schleheck, D.
    Two enzymes of the acetone degradation pathway of Desulfococcus biacutus coenzyme B12-dependent 2-hydroxyisobutyryl-CoA mutase and 3-hydroxybutyryl-CoA dehydrogenase (2018), Environ. Microbiol. Rep., 10, 283-292 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.157 expressed in Escherichia coli Rosetta 2 cells Desulfococcus biacutus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.157 (S)-3-hydroxybutanoyl-CoA + NAD+ Desulfococcus biacutus
-
 3-acetoacetyl-CoA + NADH + H+
-
 r
1.1.1.157 (S)-3-hydroxybutanoyl-CoA + NAD+ Desulfococcus biacutus KMRActS (DSM 5651)
-
 3-acetoacetyl-CoA + NADH + H+
-
 r
1.1.1.157 (S)-3-hydroxybutanoyl-CoA + NAD+ Desulfococcus biacutus KMRActS DSM 5651
-
 3-acetoacetyl-CoA + NADH + H+
-
 r
1.1.1.157 3-acetoacetyl-CoA + NADH + H+ Desulfococcus biacutus
-
 (S)-3-hydroxybutanoyl-CoA + NAD+
-
 r
1.1.1.157 3-acetoacetyl-CoA + NADH + H+ Desulfococcus biacutus KMRActS (DSM 5651)
-
 (S)-3-hydroxybutanoyl-CoA + NAD+
-
 r
1.1.1.157 3-acetoacetyl-CoA + NADH + H+ Desulfococcus biacutus KMRActS DSM 5651
-
 (S)-3-hydroxybutanoyl-CoA + NAD+
-
 r
1.1.1.157 3-hydroxybutyryl-CoA + NAD+ Desulfococcus biacutus
-
 3-acetoacetyl-CoA + NADH + H+
-
 ?
1.1.1.157 3-hydroxybutyryl-CoA + NAD+ Desulfococcus biacutus KMRActS (DSM 5651)
-
 3-acetoacetyl-CoA + NADH + H+
-
 ?
1.1.1.157 3-hydroxybutyryl-CoA + NAD+ Desulfococcus biacutus KMRActS DSM 5651
-
 3-acetoacetyl-CoA + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.157 Desulfococcus biacutus
-
-
-
1.1.1.157 Desulfococcus biacutus KMRActS (DSM 5651)
-
-
-
1.1.1.157 Desulfococcus biacutus KMRActS DSM 5651
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.157 Ni-NTA column chromatography Desulfococcus biacutus
1.1.1.157 Ni-NTA column chromatography, and Superdex 200 gel filtration Desulfococcus biacutus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.157 culture condition:acetone-grown cell
-
 Desulfococcus biacutus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.157 14.1
-
 reduction of 250 mM acetoacetyl-CoA in the presence of 0.5 mM NADH, at pH 8.0 and 30°C Desulfococcus biacutus
1.1.1.157 23.3
-
 oxidation of 0.05 mM 3-hydroxybutyryl-CoA in the presence of 5 mM NAD+, at pH 8.0 and 30°C Desulfococcus biacutus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.157 (S)-3-hydroxybutanoyl-CoA + NAD+
-
 Desulfococcus biacutus 3-acetoacetyl-CoA + NADH + H+
-
 r
1.1.1.157 (S)-3-hydroxybutanoyl-CoA + NAD+
-
 Desulfococcus biacutus KMRActS (DSM 5651) 3-acetoacetyl-CoA + NADH + H+
-
 r
1.1.1.157 (S)-3-hydroxybutanoyl-CoA + NAD+
-
 Desulfococcus biacutus KMRActS DSM 5651 3-acetoacetyl-CoA + NADH + H+
-
 r
1.1.1.157 3-acetoacetyl-CoA + NADH + H+
-
 Desulfococcus biacutus (S)-3-hydroxybutanoyl-CoA + NAD+
-
 r
1.1.1.157 3-acetoacetyl-CoA + NADH + H+
-
 Desulfococcus biacutus KMRActS (DSM 5651) (S)-3-hydroxybutanoyl-CoA + NAD+
-
 r
1.1.1.157 3-acetoacetyl-CoA + NADH + H+
-
 Desulfococcus biacutus KMRActS DSM 5651 (S)-3-hydroxybutanoyl-CoA + NAD+
-
 r
1.1.1.157 3-hydroxybutyryl-CoA + NAD+
-
 Desulfococcus biacutus 3-acetoacetyl-CoA + NADH + H+
-
 ?
1.1.1.157 3-hydroxybutyryl-CoA + NAD+
-
 Desulfococcus biacutus KMRActS (DSM 5651) 3-acetoacetyl-CoA + NADH + H+
-
 ?
1.1.1.157 3-hydroxybutyryl-CoA + NAD+
-
 Desulfococcus biacutus KMRActS DSM 5651 3-acetoacetyl-CoA + NADH + H+
-
 ?
1.1.1.157 additional information no reaction is observed with NADP+ Desulfococcus biacutus ?
-
-
1.1.1.157 additional information no reaction is observed with NADP+ Desulfococcus biacutus KMRActS (DSM 5651) ?
-
-
1.1.1.157 additional information no reaction is observed with NADP+ Desulfococcus biacutus KMRActS DSM 5651 ?
-
-

Subunits

EC Number Subunits Comment Organism
1.1.1.157 ? x * 28000, calculated from amino acid sequence Desulfococcus biacutus
1.1.1.157 ? x * 31000, His6-tagged enzyme, SDS-PAGE Desulfococcus biacutus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.157 DebiaDRAFT_04571
-
 Desulfococcus biacutus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.157 NAD+
-
 Desulfococcus biacutus
1.1.1.157 NAD+ dependent on Desulfococcus biacutus

Expression

EC Number Organism Comment Expression
1.1.1.157 Desulfococcus biacutus the enzyme expression is strongly induced in acetone-grown cells up