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Literature summary extracted from

  • Chanique, A.; Dimos, N.; Drienovska, I.; Calderini, E.; Pantin, M.; Helmer, C.; Hofer, M.; Sieber, V.; Parra, L.; Loll, B.; Kourist, R.
    A structural view on the stereospecificity of plant borneol-type dehydrogenases (2021), ChemCatChem, 13, 2262-2277 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.198 expressed in Escherichia coli BL21(DE3) cells Salvia rosmarinus
1.1.1.198 expression in Escherichia coli Salvia officinalis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.198 sitting drop vapor diffusion method, using 0.1 M HEPES/NaOH pH 7.0 to pH 7.8, 5/4 pentaerythritol propoxylate 25% (v/v), 30%, 35%, 0.1 M and 0.2 M KCl Salvia rosmarinus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.198 (+)-borneol + NAD+ Salvia rosmarinus
-
 (+)-camphor + NADH + H+
-
 ?
1.1.1.198 (+)-borneol + NAD+ Pseudomonas sp. TCU-HL1
-
 (+)-camphor + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.198 Pseudomonas sp. TCU-HL1
-
-
-
1.1.1.198 Salvia officinalis
-
-
-
1.1.1.198 Salvia rosmarinus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.198 His-Trap column chromatography Salvia rosmarinus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.198 0.0029
-
 with (-)-carveol as substrate, at pH 7.5 and 25°C Salvia rosmarinus
1.1.1.198 0.0032
-
 with propan-1-ol as substrate, at pH 7.5 and 25°C Pseudomonas sp. TCU-HL1
1.1.1.198 0.0033
-
 with cyclohexanol as substrate, at pH 7.5 and 25°C Salvia rosmarinus
1.1.1.198 0.0033
-
 with propan-1-ol as substrate, at pH 7.5 and 25°C Salvia rosmarinus
1.1.1.198 0.0039
-
 with (+)-endo-norborneol as substrate, at pH 7.5 and 25°C Salvia rosmarinus
1.1.1.198 0.0044
-
 with 3-methyl cyclohexanol as substrate, at pH 7.5 and 25°C Salvia rosmarinus
1.1.1.198 0.0046
-
 with (+)-fenchol as substrate, at pH 7.5 and 25°C Salvia rosmarinus
1.1.1.198 0.0048
-
 with (-)-exo-norborneol as substrate, at pH 7.5 and 25°C Salvia rosmarinus
1.1.1.198 0.005
-
 with (-)-carveol as substrate, at pH 7.5 and 25°C Pseudomonas sp. TCU-HL1
1.1.1.198 0.0062
-
 with heptan-2-ol as substrate, at pH 7.5 and 25°C Pseudomonas sp. TCU-HL1
1.1.1.198 0.0083
-
 with 4-methyl cyclohexanol as substrate, at pH 7.5 and 25°C Salvia rosmarinus
1.1.1.198 0.0098
-
 with heptan-2-ol as substrate, at pH 7.5 and 25°C Salvia rosmarinus
1.1.1.198 0.01
-
 with 2-methyl cyclohexanol as substrate, at pH 7.5 and 25°C Salvia rosmarinus
1.1.1.198 0.011
-
 with 4-methyl cyclohexanol as substrate, at pH 7.5 and 25°C Pseudomonas sp. TCU-HL1
1.1.1.198 0.013
-
 with octan-3-ol as substrate, at pH 7.5 and 25°C Salvia rosmarinus
1.1.1.198 0.018
-
 with 3-methyl cyclohexanol as substrate, at pH 7.5 and 25°C Pseudomonas sp. TCU-HL1
1.1.1.198 0.023
-
 with 2-methyl cyclohexanol as substrate, at pH 7.5 and 25°C Pseudomonas sp. TCU-HL1
1.1.1.198 0.024
-
 with (+)-isoborneol as substrate, at pH 7.5 and 25°C Salvia rosmarinus
1.1.1.198 0.028
-
 with (+)-endo-norborneol as substrate, at pH 7.5 and 25°C Pseudomonas sp. TCU-HL1
1.1.1.198 0.028
-
 with cyclohexanol as substrate, at pH 7.5 and 25°C Pseudomonas sp. TCU-HL1
1.1.1.198 0.03
-
 with (+)-fenchol as substrate, at pH 7.5 and 25°C Pseudomonas sp. TCU-HL1
1.1.1.198 0.03
-
 with (+/-)-borneol as substrate, at pH 7.5 and 25°C Salvia rosmarinus
1.1.1.198 0.043
-
 with octan-3-ol as substrate, at pH 7.5 and 25°C Pseudomonas sp. TCU-HL1
1.1.1.198 0.069
-
 with (-)-exo-norborneol as substrate, at pH 7.5 and 25°C Pseudomonas sp. TCU-HL1
1.1.1.198 0.115
-
 with (+)-isoborneol as substrate, at pH 7.5 and 25°C Pseudomonas sp. TCU-HL1
1.1.1.198 0.122
-
 with (+/-)-borneol as substrate, at pH 7.5 and 25°C Pseudomonas sp. TCU-HL1
1.1.1.198 9.8
-
 isoform BDH1, substrate 2-hydroxyheptanol, pH 9, temperature not specified in the publication Salvia officinalis
1.1.1.198 10
-
 isoform BDH1, substrate 2-methylcyclohexanol, pH 9, temperature not specified in the publication Salvia officinalis
1.1.1.198 10
-
 isoform BDH2, substrate rac-borneol, pH 9, temperature not specified in the publication Salvia officinalis
1.1.1.198 13
-
 isoform BDH1, substrate 6-hydroxyoctanol, pH 9, temperature not specified in the publication Salvia officinalis
1.1.1.198 16
-
 isoform BDH2, substrate (-)-carveol, pH 9, temperature not specified in the publication Salvia officinalis
1.1.1.198 24
-
 isoform BDH1, substrate isoborneol, pH 9, temperature not specified in the publication Salvia officinalis
1.1.1.198 30
-
 isoform BDH1, substrate rac-borneol, pH 9, temperature not specified in the publication Salvia officinalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.198 (+)-borneol + NAD+
-
 Salvia officinalis (+)-camphor + NADH + H+
-
 ?
1.1.1.198 (+)-borneol + NAD+
-
 Salvia rosmarinus (+)-camphor + NADH + H+
-
 ?
1.1.1.198 (+)-borneol + NAD+
-
 Pseudomonas sp. TCU-HL1 (+)-camphor + NADH + H+
-
 ?
1.1.1.198 (+)-endo-norborneol + NAD+
-
 Salvia rosmarinus (-)-exonorcamphor + NADH + H+
-
 ?
1.1.1.198 (+)-endo-norborneol + NAD+
-
 Pseudomonas sp. TCU-HL1 (-)-exonorcamphor + NADH + H+
-
 ?
1.1.1.198 (+)-fenchol + NAD+
-
 Salvia rosmarinus (-)-fenchone + NADH + H+
-
 ?
1.1.1.198 (+)-fenchol + NAD+
-
 Pseudomonas sp. TCU-HL1 (-)-fenchone + NADH + H+
-
 ?
1.1.1.198 (+)-isoborneol + NAD+
-
 Salvia rosmarinus (-)-camphor + NADH + H+
-
 ?
1.1.1.198 (+)-isoborneol + NAD+
-
 Pseudomonas sp. TCU-HL1 (-)-camphor + NADH + H+
-
 ?
1.1.1.198 (+/-)-borneol + NAD+
-
 Salvia rosmarinus (+/-)-camphor + NADH + H+
-
 ?
1.1.1.198 (+/-)-borneol + NAD+
-
 Pseudomonas sp. TCU-HL1 (+/-)-camphor + NADH + H+
-
 ?
1.1.1.198 (-)-carveol + NAD+
-
 Salvia rosmarinus ?
-
 ?
1.1.1.198 (-)-carveol + NAD+
-
 Pseudomonas sp. TCU-HL1 ?
-
 ?
1.1.1.198 (-)-cis-carveol + NAD+
-
 Salvia officinalis (-)-carvone + NADH + H+
-
 ?
1.1.1.198 (-)-exo-norborneol + NAD+
-
 Salvia rosmarinus (+)-exonorcamphor + NADH + H+
-
 ?
1.1.1.198 (-)-exo-norborneol + NAD+
-
 Pseudomonas sp. TCU-HL1 (+)-exonorcamphor + NADH + H+
-
 ?
1.1.1.198 2-hydroxyheptanol + NAD+
-
 Salvia officinalis 2-oxoheptanol + NADH + H+
-
 ?
1.1.1.198 2-methyl cyclohexanol + NAD+
-
 Salvia rosmarinus ?
-
 ?
1.1.1.198 2-methyl cyclohexanol + NAD+
-
 Pseudomonas sp. TCU-HL1 ?
-
 ?
1.1.1.198 2-methylcyclohexanol + NAD+
-
 Salvia officinalis 2-methylcyclohexanone + NADH + H+
-
 ?
1.1.1.198 3-hydroxyoctanol + NAD+
-
 Salvia officinalis 3-oxooctanol + NADH + H+
-
 ?
1.1.1.198 3-methyl cyclohexanol + NAD+
-
 Salvia officinalis 3-methycyclohexanone + NADH + H+
-
 ?
1.1.1.198 3-methyl cyclohexanol + NAD+
-
 Salvia rosmarinus ?
-
 ?
1.1.1.198 3-methyl cyclohexanol + NAD+
-
 Pseudomonas sp. TCU-HL1 ?
-
 ?
1.1.1.198 4-methyl cyclohexanol + NAD+
-
 Salvia rosmarinus ?
-
 ?
1.1.1.198 4-methyl cyclohexanol + NAD+
-
 Pseudomonas sp. TCU-HL1 ?
-
 ?
1.1.1.198 cyclohexanol + NAD+
-
 Salvia rosmarinus ?
-
 ?
1.1.1.198 cyclohexanol + NAD+
-
 Pseudomonas sp. TCU-HL1 ?
-
 ?
1.1.1.198 heptan-2-ol + NAD+
-
 Salvia rosmarinus ?
-
 ?
1.1.1.198 heptan-2-ol + NAD+
-
 Pseudomonas sp. TCU-HL1 ?
-
 ?
1.1.1.198 additional information isoforms BDH1 and BDH2 also accept monocyclic and linear substrates, clearly favoring secondary alcohols over primary alcohols. No substrate: menthol Salvia officinalis ?
-
-
1.1.1.198 additional information no activity with menthol Salvia rosmarinus ?
-
-
1.1.1.198 additional information no activity with menthol Pseudomonas sp. TCU-HL1 ?
-
-
1.1.1.198 octan-3-ol + NAD+
-
 Salvia rosmarinus ?
-
 ?
1.1.1.198 octan-3-ol + NAD+
-
 Pseudomonas sp. TCU-HL1 ?
-
 ?
1.1.1.198 propan-1-ol + NAD+
-
 Salvia rosmarinus ?
-
 ?
1.1.1.198 propan-1-ol + NAD+
-
 Pseudomonas sp. TCU-HL1 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.198 BDH
-
 Pseudomonas sp. TCU-HL1
1.1.1.198 BDH1
-
 Salvia officinalis
1.1.1.198 BDH1
-
 Salvia rosmarinus
1.1.1.198 BDH2
-
 Salvia officinalis
1.1.1.198 borneol-type dehydrogenase 1
-
 Salvia rosmarinus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.198 7.5
-
-
 Salvia rosmarinus
1.1.1.198 7.5
-
-
 Pseudomonas sp. TCU-HL1

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.198 5 10.5 isoform BDH1, 30 min, more than 50% residual activity Salvia officinalis
1.1.1.198 5 10.5 the enzyme retains over 50% activity for pH values between 5.0 and 10.5 after 30 min of incubation. After 24 h of incubation, the activity showed little variation for pH between 5.5 and 10.5 Salvia rosmarinus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.198 NAD+
-
 Salvia rosmarinus
1.1.1.198 NAD+
-
 Pseudomonas sp. TCU-HL1